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Method for producing dendrobine by inducing dendrobium candidum by jasmonic acid methyl ester

A technology of methyl jasmonate and dendrobium candidum, applied in the biological field, achieves remarkable effects, reduces production costs, and meets the effects of large-scale production

Inactive Publication Date: 2014-09-10
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the dendrobine content, chemical composition and effect of dendrobium protocorms and tissue cultured seedlings cultured in vitro are comparable to those of wild dendrobium, which still cannot effectively solve the problem of shortage of dendrobium resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Step 1. Preparation of Dendrobium candidum tissue culture seedlings: select the seeds of Dendrobium candidum from Yandang Mountain, Zhejiang, and sow them on the surface of 1 / 2MS medium with 30g / L sucrose added, at a temperature of 23°C, light intensity of 1500lx, and light-dark ratio of 10h / 14h The seed embryos are allowed to differentiate into buds, and cultivated under fluorescent lamps until the aseptic seedlings of Dendrobium candidum are about 3 to 4 cm in height, and then the aseptic seedlings of Dendrobium candidum can be induced and cultivated.

[0012] Step 2, inducing Dendrobium officinale to accumulate dendrobine: the methyl jasmonate produced by Sigma-Aldrich Company of the United States was used. First dissolve methyl jasmonate with 2% dehydrated ethanol solution, be mixed with the methyl jasmonate mother liquor that concentration is 10mmol / L, add methyl jasmonate mother liquor in the 1 / 2MS medium containing 30g / L sucrose, Until the final concentration of ...

Embodiment 2

[0014] Step 1. Preparation of Dendrobium candidum tissue culture seedlings: Select the seeds of Dendrobium candidum from Yandang Mountain, Zhejiang, and sow them on the surface of 1 / 2MS medium with 30g / L sucrose, at a temperature of 24°C, with a light intensity of 1600lx, and a light-to-dark ratio of 10h / 14h The seed embryos are allowed to differentiate into buds, cultivated under fluorescent lamps until the aseptic seedlings of Dendrobium candidum are about 3 to 4 cm in height, and then the aseptic seedlings of Dendrobium candidum are obtained through induction.

[0015] Step 2, inducing Dendrobium officinale to accumulate dendrobine: the methyl jasmonate produced by Sigma-Aldrich Company of the United States was used. First dissolve methyl jasmonate with 2% dehydrated ethanol solution, be mixed with the methyl jasmonate mother liquor that concentration is 10mmol / L, add methyl jasmonate mother liquor in the 1 / 2MS medium containing 30g / L sucrose, Until the final concentration ...

Embodiment 3

[0017] Step 1. Preparation of Dendrobium candidum tissue culture seedlings: select the seeds of Dendrobium candidum from Yandang Mountain, Zhejiang, and spread them on the surface of 1 / 2MS medium with 30g / L sucrose, at a temperature of 25°C, with a light intensity of 1700lx, and a light-to-dark ratio of 10h / 14h The seed embryos are allowed to differentiate into buds, and cultivated under fluorescent lamps until the aseptic seedlings of Dendrobium candidum are about 3 to 4 cm in height, and then the aseptic seedlings of Dendrobium candidum can be induced and cultivated.

[0018] Step 2, inducing Dendrobium officinale to accumulate dendrobine: the methyl jasmonate produced by Sigma-Aldrich Company of the United States was used. First dissolve methyl jasmonate with 2% dehydrated ethanol solution, be mixed with the methyl jasmonate mother liquor that concentration is 10mmol / L, add methyl jasmonate mother liquor in the 1 / 2MS medium containing 30g / L sucrose, Until the final concentr...

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Abstract

The invention provides a method for producing dendrobine by inducing dendrobium candidum by jasmonic acid methyl ester. The method comprises the following steps: (1) sowing dendrobium candidum seeds on the surface of a 1 / 2MS culture medium and culturing under the conditions that the temperature is 23-25 DEG C, the light intensity is 1500-1700 lx and the light-dark ratio is 10h / 14h to obtain tissue culture seedlings of dendrobium candidum; and (2) dissolving jasmonic acid methyl ester in a 2% absolute ethyl alcohol solution to form a jasmonic acid methyl ester mother solution with the concentration of 10mmol / L, adding the jasmonic acid methyl ester mother solution into the 1 / 2MS culture medium till the final concentration of jasmonic acid methyl ester is 48-52 micromole / L, transplanting the tissue culture seedlings of dendrobium candidum to the 1 / 2MS culture medium with the jasmonic acid methyl ester mother solution and culturing for 29-31 days under the conditions that the temperature is 26-28 DEG C, the relative humidity is 66%-68%, the light intensity is 1800-2000 lx and the light-dark ratio is 12h / 12h. The method provided by the invention has the advantages that the efficiency of accumulation of dendrobine in dendrobium candidum can be increased by a large margin by adding a small quantity of jasmonic acid methyl ester during the tissue culture process, and the method is small in investment and convenient and easy to carry out and has remarkable effects.

Description

technical field [0001] The invention provides a method for using methyl jasmonate to induce dendrobium officinale to produce dendrobine, which belongs to the field of biotechnology. Background technique [0002] Dendrobine is one of the most important physiologically active components of Dendrobium officinale, and its pharmacological strength has a certain relationship with the alkaloid content. Studies on the biological function and pharmacological effects of Dendrobium officinale have shown that it has the functions of enhancing immunity, anti-cancer, lowering blood pressure, as well as sedative and analgesic effects. [0003] Dendrobium officinale has low reproductive rate, slow growth, over-harvesting and utilization, and damage to the ecological environment under natural conditions, which lead to the endangerment of wild resources and limit the production and further development and utilization of dendrobium. With the maturation of tissue culture technology, scholars a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 袁佐清张建勇刘涛林聪
Owner SHANDONG UNIV OF TECH
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