A method for increasing the yield and activity of Paecilomyces cicadae liquid fermentation product

A Paecilomyces cicadae, liquid fermentation technology, applied in the field of fermentation engineering, to achieve the effects of increasing the content, increasing the activity of intracellular polysaccharides, and increasing the activity of exopolysaccharides

Active Publication Date: 2016-01-27
浙江好溢笙生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on significantly promoting the growth of edible and medicinal fungi or increasing the production of active metabolites by adding appropriate amount of traditional Chinese medicine ingredients or oils to the medium has been reported many times at home and abroad, but the research on the liquid fermentation of Paecilomyces cicadae Application not yet reported

Method used

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  • A method for increasing the yield and activity of Paecilomyces cicadae liquid fermentation product
  • A method for increasing the yield and activity of Paecilomyces cicadae liquid fermentation product
  • A method for increasing the yield and activity of Paecilomyces cicadae liquid fermentation product

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Effect test

Embodiment 1

[0034] 1. Fermentation culture

[0035] (1) Shake flask seed culture

[0036] 1L liquid seed medium: glucose 20g / L, yeast powder 5g / L, KH 2 PO 4 1g / L and MgSO 4 0.5g / L, the balance is water, the pH is natural, and sterilized at 121°C for 20min.

[0037] Cultivation method: Paecilomyces cicadae species activated in PDA slant medium, take 5mm 2 (that is, the size of soybeans) were inserted into the sterilized liquid seed medium, the filling capacity of the 500mL Erlenmeyer flask was 150mL, and cultured on a shaking table at 25°C and 120r / min for 3 days to obtain the cultivated seed liquid.

[0038] (2) Preparation of traditional Chinese medicine compound water extract

[0039] Weigh 1 g of compound Chinese herbal medicine (crushed, passed through 60 meshes), which consists of the following Chinese herbal medicines: by weight, 10 parts of Coix seed, 40 parts of Astragalus membranaceus, 20 parts of Lycium barbarum and 30 parts of Atractylodes macrocephala. Add 10 times of wa...

Embodiment 2

[0059] 1 Fermentation culture

[0060] (1) Shake flask seed culture

[0061] 1L liquid seed medium: glucose 20g / L, yeast powder 5g / L, KH 2 PO 4 1g / L and MgSO 4 0.5g / L, the balance is water, the pH is natural, and sterilized at 121°C for 20min.

[0062] Cultivation method: Paecilomyces cicadae species activated in PDA slant medium, take 5mm 2 (that is, the size of soybeans) were inserted into the sterilized liquid seed medium, the filling capacity of the 500mL Erlenmeyer flask was 150mL, and cultured on a shaking table at 25°C and 120r / min for 3 days to obtain the cultivated seed liquid.

[0063] (2) Preparation of traditional Chinese medicine compound water extract

[0064] Weigh 1 g of compound Chinese herbal medicines (crushed, passed through 60 meshes), which consist of the following Chinese herbal medicines: by weight, 20 parts of Coix seed, 30 parts of Astragalus membranaceus, 30 parts of Lycium barbarum and 20 parts of Atractylodes macrocephala. Add 10 times of wat...

Embodiment 3

[0071] 1 Fermentation culture

[0072] (1) Shake flask seed culture

[0073] 1L liquid seed medium: glucose 20g / L, yeast powder 5g / L, KH 2 PO 4 1g / L and MgSO 4 0.5g / L, the balance is water, the pH is natural, and sterilized at 121°C for 20min.

[0074] Cultivation method: Paecilomyces cicadae species activated in PDA slant medium, take 5mm 2 (that is, the size of soybeans) were inserted into the sterilized liquid seed medium, the filling capacity of the 500mL Erlenmeyer flask was 150mL, and cultured on a shaking table at 25°C and 120r / min for 3 days to obtain the cultivated seed liquid.

[0075] (2) Preparation of traditional Chinese medicine compound water extract

[0076] Weigh 1 g of compound Chinese herbal medicine (crushed, passed through 60 meshes), which consists of the following Chinese herbal medicines: by weight, 30 parts of coix seed, 10 parts of astragalus, 40 parts of wolfberry and 20 parts of Atractylodes macrocephala. Add 10 times of water to soak for 0.5h...

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Abstract

The invention discloses a method for improving the yield and product activity of a liquid fermentation product of Paecilomyces cicadae, which comprises the following steps: (1) extracting traditional Chinese medicinal materials composed of 10-40 parts by weight of coix seed, 10-40 parts by weight of radix astragali, 10-40 parts by weight of Chinese wolfberry and 10-40 parts by weight of rhizoma atractylodis macrocephalae through a water solution, thus obtaining a traditional Chinese medicine compound water extract liquid; (2) adding the traditional Chinese medicine compound water extract liquid prepared in the step (1), rhizoma atractylodis macrocephalae lactone and Tween-80 into a fermentation basal culture medium, thus obtaining a Paecilomyces cicadae fermentation culture medium; and (3) inoculating a Paecilomyces cicadae strain into the Paecilomyces cicadae fermentation culture medium prepared in the step (2), performing liquid fermentation to obtain a fermentation liquid, and separating to obtain a fermentation product. According to the invention, particular kinds of traditional Chinese medicine compound water extract liquid, rhizoma atractylodis macrocephalae lactone and Tween-80 are added, so that the biomass and polysaccharide yield of the Paecilomyces cicadae can be obviously improved, and meanwhile, the activity of the polysaccharide is also improved obviously.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and in particular relates to a method for increasing the yield and activity of Paecilomyces cicadae liquid fermentation products. Background technique [0002] Edible and medicinal fungus Paecilomycescicadae (Mique1) Samson, also known as female cicada, belongs to the same genus of Cordyceps as Cordyceps sinensis and Cordyceps militaris, and is an asexual strain of a precious traditional Chinese medicinal material, Cordyceps sobolifera. The active ingredients and efficacy of Paecilomyces cicadae are similar to those of Cordyceps sinensis, containing nucleosides, myriocin, ergosterol, cordycepic acid, polysaccharides, various essential amino acids, mannitol and alkaloids and other secondary metabolites ( He Liang, Ma Suyun, Cheng Junwen, et al. Research progress on bioactive substances of the medicinal fungus Paecilomyces cicadae [J]. Journal of Food and Biotechnology, 2012,31(1):8-15.). M...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04C12R1/79
Inventor 贺亮程俊文韩素芳韦朝阳李卫旗李海波付立忠魏海龙胡传久
Owner 浙江好溢笙生物科技有限公司
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