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Gene ghass1 for improving salt tolerance of Escherichia coli and its preparation and protein encoded by it

A technology of Escherichia coli and salt tolerance, applied in the field of new gene preparation, can solve the problems of related molecular biology that have not been seen

Inactive Publication Date: 2016-03-02
LANGFANG NORMAL UNIV
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Problems solved by technology

At present, there are no reports of related molecular biology research in plants

Method used

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  • Gene ghass1 for improving salt tolerance of Escherichia coli and its preparation and protein encoded by it
  • Gene ghass1 for improving salt tolerance of Escherichia coli and its preparation and protein encoded by it
  • Gene ghass1 for improving salt tolerance of Escherichia coli and its preparation and protein encoded by it

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Embodiment Construction

[0122] The embodiments of the present invention will be further described below in conjunction with the accompanying drawings, but this is not a limitation of the present invention. The scope of protection of the present invention is based on the contents of the claims. Any replacement of equivalent technical means based on the specification does not depart from this scope of protection for inventions.

[0123] The gene of this embodiment and the amino acid sequence encoded by it are as described above, and the preparation method of the gene is as follows:

[0124] The preparation method of the gene GhASS1 that improves the salt tolerance of Escherichia coli uses 5'-ACCATGGGCGAGCTCGGTACCATGGCTCAGTTCAAAG-3' as the upstream primer 5ZWASS, and 5'-TTGCGGACTCTAGAGGATCCCACTTACATACCTTTGTTAAGCATTGC-3' as the downstream primer 3ZWASS, and the reverse transcription of RNA in cotton plant roots The synthesized cDNA was used as a template and obtained by PCR amplification.

[0125] The p...

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Abstract

The invention belongs to preparation of new genes and particularly relates to a gene GhASS1 for improving salt tolerance of Escherichia coli and a preparation of the gene GhASS1 as well as a protein coded by the gene GhASS1. The gene is obtained through a PCR (Polymerase Chain Reaction) amplification method by taking 5'-ACCATGGGCGAGCTCGGTACCATGGCTCAGTTCAAAG-3' as an upstream primer 5ZWASS and 5'-TTGCGGACTCTAGAGGATCCCACTTACATACCTTTGTTAAGCATTGC-3' as a downstream primer 3ZWASS and by using cDNA (Complementary Deoxyribonucleic Acid) synthesized by reverse transcription of RNA (Ribonucleic Acid) in a cotton plant root as a template. The gene provided by the invention fills the blank that in the prior art, no related molecular biology study reports of argininosuccinate synthase in plants are found. The gene has the advantages of improving the growing activity of recombinant bacteria under salt stress due to expression of the gene GhASS1 in the recombinant bacteria and enhancing the salt tolerance of the strain.

Description

technical field [0001] The invention belongs to the preparation of new genes, in particular to a gene GhASS1 for improving the salt tolerance of Escherichia coli, its preparation and the protein encoded by it. Background technique [0002] The urea cycle (UreaCycle), also known as the ornithine cycle, is the first cyclic metabolic pathway discovered. In mammals, 2 molecules of ammonia (1 molecule of ammonia is free, 1 molecule of ammonia from aspartic acid) and 1 molecule of CO 2 The cyclic metabolic pathway to generate one molecule of urea is the process of generating urea, the final product of nitrogen metabolism, so it has the effect of removing ammonia toxicity. In addition, it not only converts ammonia and CO 2 It is synthesized into urea, and a molecule of fumaric acid is generated, which links the urea cycle with the citric acid cycle. The liver is the main organ for urea synthesis in mammals. In clinical practice, any problem with any step of the urea cycle may ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/52C12N15/10C12N9/00
Inventor 孙艳香王慧飞冯雪贾永红张一名
Owner LANGFANG NORMAL UNIV
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