Method For Detecting Mercury Ions In Solution, And Kit

A kit and mercury ion technology, applied in the fields of biochemistry and electrochemistry, can solve the problems of complex fluorescence instruments, which are not suitable for on-site detection, etc., and achieve the effects of improving detection sensitivity, rapid detection, and easy operation

Inactive Publication Date: 2014-09-24
THE HONG KONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires multiple steps and complex fluorescence instrumentation, making it unsuitable for on-site detection

Method used

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  • Method For Detecting Mercury Ions In Solution, And Kit
  • Method For Detecting Mercury Ions In Solution, And Kit
  • Method For Detecting Mercury Ions In Solution, And Kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] In 1×NEBuffer1 buffer, e-T-rich single-stranded DNA was mixed with triplicate 10 μL, Hg 2+ Samples of the test solutions with different concentrations (referred to as Sample 1, Sample 2, and Sample 3, respectively) were mixed at room temperature so that the concentration of e-T-rich reached 2 μM. The mixture was left at room temperature for 20 minutes. Then 20 units of Exo III were added to 50 μL and left at 45°C for 20 minutes. It was then heated to 80°C for 10 minutes to inactivate the Exo III. Finally, cool slowly to room temperature (approximately 20 min) for PAGE analysis and electrochemical detection on ITO electrode chips. The results of this example are listed in the following table:

[0113] Sample No

Embodiment 2

[0115] Will contain 2 μM e-T-rich probe, 20 units of Exo III and 10 μL containing different concentrations of Hg 2+ The reaction mixtures of the samples to be tested were incubated in 1x NEBuffer1 buffer at 45 °C for 20 min. It was then heated to 80°C for 10 minutes to inactivate the Exo III. Finally, cool slowly to room temperature (approximately 20 min) for PAGE analysis and electrochemical detection on ITO electrode chips. The results of this example are listed in the following table:

[0116] Sample No

Current size

detected Hg 2+ concentration

Sample 1

1.03

0nM

Sample 2

1.96

0.5nM

Sample 3

2.61

1nM

Sample 4

3.33

5nM

Sample 5

4.18

10nM

Sample 6

4.85

20nM

Sample 7

5.77

40nM

Sample 8

6.66

80nM

[0117] in conclusion:

[0118] The above test examples and examples prove that the detection solution of the present invention contains...

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Abstract

The invention provides a method for detecting mercury ions in a solution, and a kit. The method comprises: 1) mixing the solution with single-stranded DNA containing thymine to form a mixture so that the single-stranded DNA containing the thymine forms double-stranded DNA through T-Hg2+-T base pairs wherein at least one deoxyribonucleotide of the single-stranded DNA containing the thymine is connected with a signal generating molecule; 2) contacting the double-stranded DNA obtained from the step 1) with exonuclease specifically different from the double-stranded DNA so that the exonuclease cuts the double-stranded DNA to release the deoxyribonucleotides connected with the signal generating molecules; and 3) detecting the signal generating molecules connected with the deoxyribonucleotides released in the detection step 2). The method has high specificity and sensitivity, does not need fixed DNA, is convenient to operate and can realize rapid detection.

Description

technical field [0001] The invention relates to the fields of biochemistry and electrochemistry, in particular to a method and a kit for detecting mercury ions in a solution. Background technique [0002] There are specific binding interactions between metal ions and nucleic acid bases. For example, such as Ag + , Hg 2+ , Cu 2+ , Ni 2+ Isometallic ions can bind to specific natural or synthetic nucleotides through coordinative forces to form metal ion-mediated base pairs in which the hydrogen bonds in Watson-Crick (W-C) type base pairs are replaced by metal-base bonds replace. This novel interaction of nucleic acids with metal ions holds great promise in a variety of applications, including the preparation of DNA-based biosensors, the design of molecular-level logic gates, and the regulation of DNA function. [0003] Mercury ions (Hg 2+ ) is a bioaccumulative and highly toxic environmental pollutant that can cause serious human health problems such as DNA damage, brain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N27/48
CPCC12Q1/6816
Inventor 邢怡铭宣锋罗晓腾
Owner THE HONG KONG UNIV OF SCI & TECH
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