A biosensor for detecting organophosphorus pesticides and its preparation method

A technology of biosensors and organophosphorus pesticides, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of affecting sensor sensitivity, small detection range, and low sensitivity, and achieve the goal of improving fixation efficiency, amplifying electrical signals, and improving sensitivity Effect

Active Publication Date: 2022-03-11
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing biosensors have disadvantages such as low sensitivity, small detection range, and poor stability.
Most of the sensors have low sensitivity because they cannot amplify the electrical signal generated by the oxidation of choline generated by the hydrolysis of acetylcholinesterase by acetylcholinesterase, and acetylcholinesterase, as a protein, will hinder the connection between the enzyme and the electrode. The electron transfer between them affects the sensitivity of the sensor

Method used

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  • A biosensor for detecting organophosphorus pesticides and its preparation method
  • A biosensor for detecting organophosphorus pesticides and its preparation method
  • A biosensor for detecting organophosphorus pesticides and its preparation method

Examples

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preparation example Construction

[0028] The invention provides the preparation method of the biosensor, which comprises the following steps: after cleaning the electrode base sheet, fixing conductive materials and acetylcholinesterase at different positions of the electrode base sheet.

[0029] In the present invention, the cleaning preferably includes acetone cleaning, absolute ethanol cleaning and deionized water ultrasonic cleaning carried out in sequence; the time for the acetone cleaning, absolute ethanol cleaning, and deionized water cleaning is preferably 5 min respectively; A drying step is also preferably included after the cleaning. In the present invention, the drying is preferably nitrogen blowing.

[0030] In the present invention, when the conductive material and acetylcholinesterase are fixed on both sides of the electrode base sheet respectively, in a specific embodiment of the present invention, silver nanowires are sequentially added dropwise on one side of the electrode base sheet Solution...

Embodiment 1

[0039] (1) The glass sheet is cleaned with acetone, absolute ethanol, and deionized water ultrasonically, and blown dry with nitrogen;

[0040] (2) Add 4 μL of silver nanowire solution and 4 μL of graphene solution dropwise to one side of the glass plate, and then vacuum dry for 20 minutes;

[0041] (3) On the other side of the glass slide, 4 μL of titanium oxide, 4 μL of 5 wt % CS and acetylcholinesterase solution were added dropwise in sequence, and dried in the air to obtain a sensor detection electrode.

[0042] The detection electrode is placed in an electrochemical workstation for detection.

Embodiment 2

[0044] (1) Ultrasonic cleaning of the purchased double-sided conductive glass ITO with acetone, absolute ethanol, and deionized water for 5 minutes respectively; after cleaning, blow dry with nitrogen.

[0045] (2) 4 μL of silver nanowire solution and 4 μL of graphene solution were sequentially added dropwise to the conductive surface on one side of the glass plate, and then vacuum dried for 20 min.

[0046] (3) Electroplating a CS layer on the other conductive surface, in 0.2wt% CS solution, voltage -2.5V, time 20s.

[0047] (4) Add 4 μL ChE solution (in PBS buffer, containing

[0048] 1% bovine serum albumin), air-dried, and stored at 4°C.

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Abstract

The invention provides a biosensor for detecting organophosphorus pesticides and a preparation method thereof, belonging to the technical field of pesticide detection. The biosensor includes an electrode substrate, a conductive material fixed on the electrode substrate, and acetylcholinesterase; The conductive material and acetylcholinesterase are respectively fixed on different positions of the electrode base sheet. The biosensor separates enzyme immobilization from signal acquisition and amplification channels, separates and immobilizes acetylcholinesterase from electrode materials without interfering with each other, improves enzyme immobilization efficiency, stability, and electrode contact area, and further amplifies the electric current. signal, improving the sensitivity of the sensor.

Description

technical field [0001] The invention belongs to the technical field of pesticide detection, and in particular relates to a biosensor for detecting organophosphorus pesticides and a preparation method thereof. Background technique [0002] To protect crops from insect pests, especially in developing countries, organophosphorus pesticides (OPs) such as dichlorvos, malathion, parathion, paraoxon and It has the advantages of low cost, strong selectivity, and little harm; it is widely used to fight against the invasion of agricultural pests all over the world. However, the excessive use of pesticides leads to the long-term existence of residual pesticides in the environment, which will pose a serious threat to the health of animals and humans. The phosphate group in organophosphorus pesticides can irreversibly bind to the serine (Ser) hydroxyl group in the active site of acetylcholinesterase (AChE), therefore, appropriate measures should be taken to rapidly detect toxic OPs. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3278G01N27/48
Inventor 杨连乔汪波胡华赢王朋厂
Owner SHANGHAI UNIV
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