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Cultivation method for polyploid clematis ranunculoides

A technology of buttercup clematis and cultivation methods, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of reducing the proportion, improving ornamental value and commercial value, and improving induction efficiency

Inactive Publication Date: 2014-10-08
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of polyploid induction in Clematis ranunculus has not yet been reported publicly.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Screen the buttercup clematis seeds, remove the empty seeds and impurities, and reserve the full seeds for later use to ensure the quality of the seeds and increase the germination rate of the seeds, and then use the high manganese content of 0.5% for the buttercup clematis Potassium acid potassium solution was soaked and disinfected for 6 hours, rinsed with water, treated with 1% mercury liter for 4 minutes, and then the treated seeds were inserted into the growth medium: MS+6-BA 2mg / L+NAA 0.5mg / L+AgNO 3 3mg / L, under the conditions of temperature 25℃, light intensity 1700Lx, relative humidity 70%;

[0015] (2) When the seeds in step (1) grow radicles or germs of 4 mm, soak the seeds with a sterile colchicine solution with a mass concentration of 0.05% for 18 hours, then rinse them with sterile water, and then absorb the water Inoculated in the above-mentioned growth medium, cultured for 32 days at a temperature of 26°C, a light intensity of 1500Lx, and a relative ...

Embodiment 2

[0018] (1) Screen the full and undamaged buttercup clematis seeds harvested in the same year, remove empty seeds and impurities, and reserve full seeds for later use to ensure seed quality and increase seed germination rate, and then use buttercup clematis seeds Soak and disinfect in 0.5% potassium permanganate solution for 6 hours, rinse with water, treat with 1% mercury chloride for 5 minutes, and then insert the treated seeds into the growth medium: MS+6-BA 2mg / L+NAA 0.5mg / L+AgNO 3 3mg / L, cultured and accelerated germination under the conditions of temperature of 22°C, light intensity of 1300Lx, and relative humidity of 75%;

[0019] (2) When the seeds in step (1) grow radicles or germs of 5 mm, soak the seeds with a sterile colchicine solution with a mass concentration of 0.1% for 12 hours, then rinse them with sterile water, and then absorb the water Inoculated in the above-mentioned growth medium, cultured for 35 days at a temperature of 24°C, a light intensity of 130...

Embodiment 3

[0022] (1) Screen the buttercup clematis seeds, remove the empty seeds and impurities, and reserve the full seeds for later use to ensure the quality of the seeds and increase the germination rate of the seeds, and then use the high manganese content of 0.5% for the buttercup clematis Potassium acid potassium solution for 6 hours, then rinsed with water, treated with 1% mercury liter for 3 minutes, and then inserted the treated seeds into the growth medium: MS+6-BA 2mg / L+NAA 0.5mg / L+AgNO 3 3mg / L, cultured and accelerated germination at a temperature of 26°C, a light intensity of 1500Lx, and a relative humidity of 65%;

[0023] (2) When the seeds in step (1) grow radicles or germs of 6 mm, soak the seeds with a sterile colchicine solution with a mass concentration of 0.15% for 6 hours, then rinse them with sterile water, and then absorb the water Inoculated in the above-mentioned growth medium, cultured for 30 days at a temperature of 22°C, a light intensity of 1700Lx, and a r...

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Abstract

The invention provides a cultivation method for polyploid clematis ranunculoides. The method comprises the following steps: screening and treating seeds, inoculating the seeds into a growth culture medium for cultivation and germination acceleration, soaking the seeds by using a colchicine solution, washing the seeds by using sterile water, inoculating the seeds into the growth culture medium, and then continuing to cultivate seedlings which are obviously variable in shape in the growth culture medium to induce new bud seedlings; cyclically performing the steps to propagate the variable seedlings of five generations; performing polyploid detection, transferring the seedlings identified as polyploid to a rooting culture medium for rooting culture, and transplanting after the base parts of the seedlings are rooted to obtain a clematis ranunculoides polyploidy plant. According to the method, in a mode of combining tissue culture and colchicine induction, the chimera proportion can be effectively reduced, the characteristic that the flower of the clematis ranunculoides is small is changed, a new variety of a large flower type is bred, the merits of a female parent are maintained, the polyploid induction efficiency of the clematis ranunculoides can be effectively improved, and the polyploid ratio reaches 23.6 percent.

Description

technical field [0001] The invention relates to a method for inducing plant polyploids, in particular to a method for inducing and cultivating polyploid buttercup clematis by using chemical agents, and belongs to the technical field of plant breeding. Background technique [0002] Buttercup clematis ( Clematis ranunculoides Franch . ) is a herbaceous vine of Ranunculaceae, distributed in Southwest China. The flowers are dense, purple-red, and the flowering period is from September to October, which coincides with the National Day and Mid-Autumn Festival. It has high ornamental value and can be used as potted flowers or climbing green plants. At the same time, the whole herb is used as medicine to treat various diseases. Therefore, clematis ranunculus is a medicinal ornamental plant with great development value. [0003] However, the flowers of Buttercup clematis are small, and if new varieties with large flowers can be bred through genetic improvement, their ornamental ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李叶芳李枝林关文灵牛红彬
Owner YUNNAN AGRICULTURAL UNIVERSITY
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