A high-throughput single-cell reverse transcription PCR analysis device
A single-cell, high-throughput technology, applied in the fields of cell biology, microfluidics, and molecular biology, it can solve the problems of difficult operation, complicated equipment, difficult long-term stable capture, etc., and achieve high-throughput analysis and easy processing. effect achieved
Active Publication Date: 2016-02-24
XI AN JIAOTONG UNIV
View PDF2 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Conventional cell capture methods include optical tweezers, magnetic force, dielectrophoresis and mechanical force capture, etc. Most of these methods have the disadvantages of complex equipment required, difficult operation or long-term stable capture
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0019] The present invention will be clearly described below in conjunction with the accompanying drawings and embodiments.
[0020] refer to figure 1 with figure 2 , a high-throughput single-cell reverse transcription PCR device, including reagent inlet 1, sample inlet 2, reagent inlet 1, sample inlet 2 are connected through microvalve 6 and the inlet of microchannel 7, and the outlet of microchannel 7 is through micro The valve 6 is connected to one end of two or more single-cell capture chambers 3 in series, the other end of the single-cell capture chambers 3 in series is connected to the waste liquid outlet 11 through a microvalve 6, and the single-cell capture chambers 3 are connected through a microvalve 6, Each single cell capture chamber 3 includes an inverted triangular auxiliary capture structure 8 and a capture trap 9, the capture trap 9 is located at the lower central part of the single cell capture chamber 3, the inverted triangular auxiliary capture structure 8...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a high-flux single cell reverse transcription PCR analysis device. A reagent inlet and a sample inlet are connected with a micro channel through micro valves; the micro channel is connected with one ends of more than two serially-connected single cell capturing cavities, and the other ends of the serially-connected single cell capturing cavities are connected with a waste liquid outlet; each single cell capturing cavity comprises an inverted triangular auxiliary capturing structure and a capturing trap, wherein the capturing trap is located at the lower part of the center of the single cell capturing cavity, the inverted triangular auxiliary capturing structure is located above the capturing trap, the capturing trap is connected with a reverse transcription cavity, and the reverse transcription cavity is connected with a nucleic acid amplification cavity; cell suspension liquid injected from the sample inlet enters the serially-connected cell capturing cavities, cells are captured into the cell capturing traps under the action of water power, and then, a cell lysis solution is added from the reagent inlet and sequentially enters the cell capturing traps, so that reverse transcription, nucleic acid amplification and detection are finished. The high-flux single cell reverse transcription PCR analysis device is simple in structure and easy to realize.
Description
technical field [0001] The invention relates to the fields of molecular biology, cell biology and microfluidic technology, in particular to a high-throughput single-cell reverse transcription PCR device. Background technique [0002] Cells are the basic structural and functional units of organisms, and the physiological functions of organisms and all life phenomena are expressed on the basis of cells. Nucleic acid is the genetic material of living organisms, which is closely related to cell division, growth, differentiation and apoptosis. PCR (polymerase chain reaction) technology is an important nucleic acid detection technology, which is used to amplify specific DNA fragments; reverse transcription PCR is a means to analyze the gene expression of cells starting from RNA. Due to the advantages of high sensitivity and high specificity, PCR technology is widely used in the fields of biology, medicine, agriculture and food safety. [0003] Traditional gene amplification and ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M1/00
CPCB01L7/52C12M23/16C12M45/00
Inventor 彭年才胡飞周赵来李政赵书豪
Owner XI AN JIAOTONG UNIV