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Fungus capable of degrading phthalic acid ester and pesticide simultaneously and application thereof

A technology of phthalates and pesticides, applied in the field of fungi that degrade phthalates and pesticides at the same time, to achieve a good degradation effect

Inactive Publication Date: 2014-10-08
SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The primary purpose of the present invention is to overcome the deficiencies in the prior art PAEs and pesticide compound polluted farmland soil bioremediation technology, and provide a fungus that can simultaneously degrade phthalates and pesticides

Method used

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  • Fungus capable of degrading phthalic acid ester and pesticide simultaneously and application thereof
  • Fungus capable of degrading phthalic acid ester and pesticide simultaneously and application thereof
  • Fungus capable of degrading phthalic acid ester and pesticide simultaneously and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Screening and isolation of Geotrichum sp. DY4 and its degradation performance to PAEs and pesticides

[0020] (1) Screening and isolation of Geotrichum sp. DY4

[0021] Soil samples were taken from the plow layer (0-20 cm) of a farmland polluted by PAEs and pesticides in a certain place in Guangdong Province. Weigh 10g of the soil sample, put it into a 250mL Erlenmeyer flask with 90mL of deionized water and 4-5 glass beads, vibrate at 28°C and 180rpm for 30min, and let it stand for 30min. Get 10mL of supernatant, add 140mL of inorganic salt medium containing composite PAEs and pesticides to the supernatant [the composition of inorganic salt medium is as follows: (NH 4 ) 2 SO 4 1.0g / L, KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O0.2g / L, CaCl 2 0.1g / L, FeCl 3 0.01g / L, adjust the pH value to 7.0, sterilize by high-pressure steam at 121°C for 20min, and carry out gradient acclimatization three times in the same below. The concentrations of 3 PAEs (DMP, DBP and DEHP,...

Embodiment 2

[0045] Example 2: Effects of growth factors on the degradation effect of Geotrichum sp. DY4

[0046] Select the external carbon source (glucose), the concentration of organic pollutants (DBP and fenvalerate) and pH as the influencing factors, and use an orthogonal design (as shown in Table 3) to optimize the degradation conditions of DBP and fenvalerate. Inorganic salt medium was used, 100mL per bottle, and the concentration values ​​of the two organic pollutants were shown in Table 3. Refer to Example 1 for the expanded culture of bacterial strains. By adding a concentration of 1mol L -1 The NaOH solution was used to adjust the pH value, cultured at 28°C / 120rpm for 7 days, and an uninoculated inorganic salt medium was used as a control. Determine the content of organic matter and the pH value of the bacterial solution, calculate the degradation rate and find out the optimal degradation conditions.

[0047] Using the range analysis method, the results in Tables 4 to 7 show ...

Embodiment 3

[0061] Example 3: Degradation effect of Geotrichum sp. DY4 on DBP and fenvalerate in sterilized soil

[0062] The clean soil used in the test was taken from the 0-20 cm plow layer of the rice test field of South China Agricultural University. The soil indicators are as follows: 2.20% organic matter, 0.10% total nitrogen, and 130 mg kg -1 , Available phosphorus 114mg·kg -1 , available potassium 284mg·kg -1 , the pH value is 6.08, which is medium loam. After being air-dried, ground by a soil grinder, and passed through a 100-mesh sieve, the base soil was obtained. Set up 5 concentration gradients for DBP and fenvalerate, 0, 25, 50, 75, 100 mg·kg -1 , added to the base soil to obtain artificially contaminated soil. 40g of each artificially contaminated soil sample was set up in 3 parallels, put into a clean glass petri dish, and all samples were sterilized twice a day by high-pressure steam at 121°C for 20 minutes each time. After the strains were expanded and cultured with ...

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Abstract

The invention discloses a fungus capable of degrading phthalic acid ester and pesticide simultaneously and application thereof. The name of the fungus is Geotrichum sp.DY4, and the fungus is preserved in China Center for Type Culture Collection in Wuhan University in Wuhan, China on May 11, 2014 with the preservation number of CCTCC NO:M2014194. The fungus disclosed by the invention can degrade phthalic acid ester and pesticide simultaneously, can be used for degrading pollution caused by multiple PAEs and pesticide mixtures and also can be used for soil biological remediation, and a new genetic resource is provided for study of a degradation mechanism on PAEs and pesticide in soil.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, in particular to a fungus capable of simultaneously degrading phthalates and pesticides and its application. Background technique [0002] In order to meet the growing demand for various materials, human beings use a large amount of agricultural chemicals such as mulch film, organic pesticides, and chemical fertilizers in agricultural production, resulting in the compound pollution of various organic substances to the soil. The mulching film in the farmland soil has the effect of heat preservation and moisture retention for crops, especially in the process of growing vegetables and fruits in greenhouses. It plays a very important role. However, the plasticizer in the mulch - phthalic acid esters (PAEs) is difficult to firmly bond with the plastic itself, and its stability is relatively poor. PAEs are easy to seep out of the plastic mulch, causing s...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C12R1/645
Inventor 蔡信德王冬莹吴文成方晓航李诗殷
Owner SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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