Fungus capable of degrading phthalic acid ester and pesticide simultaneously and application thereof

A technology of phthalates and pesticides, applied in the field of fungi that degrade phthalates and pesticides at the same time, to achieve a good degradation effect

Inactive Publication Date: 2014-10-08
SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The primary purpose of the present invention is to overcome the deficiencies in the prior art PAEs and pesticide compound

Method used

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  • Fungus capable of degrading phthalic acid ester and pesticide simultaneously and application thereof
  • Fungus capable of degrading phthalic acid ester and pesticide simultaneously and application thereof
  • Fungus capable of degrading phthalic acid ester and pesticide simultaneously and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1 Screening and isolation of Geotrichum sp. DY4 and its degradation performance on PAEs and pesticides

[0020] (1) Screening and isolation of Geotrichum sp. DY4

[0021] Soil samples were taken from the cultivated layer (0-20cm) of farmland contaminated by PAEs and pesticides in a certain place in Guangdong Province. Weigh 10 g of the soil sample, fill it with a 250 mL Erlenmeyer flask containing 90 mL of deionized water and 4 to 5 glass beads, shake at 28° C. and 180 rpm for 30 minutes, and let it stand for 30 minutes. Take 10 mL of the supernatant and add 140 mL of the inorganic salt medium containing compound PAEs and pesticides to the supernatant [the composition of the inorganic salt medium is as follows: (NH 4 ) 2 SO 4 1.0g / L, KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O0.2g / L, CaCl 2 0.1g / L, FeCl 3 0.01g / L, adjust the pH to 7.0, autoclave at 121°C for 20 minutes, and perform 3 gradient acclimatization in the following]. The concentrations of 3 PAEs (DMP, DBP and DEHP, t...

Example Embodiment

[0045] Example 2: Influence of growth factors on the degradation effect of Geotrichum sp. DY4

[0046] The concentration and pH of the external carbon source (glucose), organic pollutants (DBP and fenvalerate) were selected as influencing factors, and orthogonal design (as shown in Table 3) was used to optimize the degradation conditions of DBP and fenvalerate. Inorganic salt medium is used, 100 mL per bottle. Table 3 shows the concentration values ​​reached by the two organic pollutants. Refer to Example 1 for the expansion and cultivation of strains. By adding a concentration of 1mol·L -1 Adjusted the pH value with the NaOH solution, cultured at 28°C / 120rpm for 7 days, and used uninoculated inorganic salt medium as control. Determine the organic content and pH value of the bacterial solution, calculate the degradation rate and find the optimal degradation conditions.

[0047] Using the range analysis method, the results of Tables 4 to 7 show that the best test conditions for Ge...

Example Embodiment

[0061] Example 3: The degradation effect of Geotrichum sp. DY4 on DBP and fenvalerate in sterilized soil

[0062] The clean soil used in the experiment was taken from the 0-20cm cultivated layer of the rice experiment field of South China Agricultural University. The soil indicators are as follows: organic matter 2.20%, total nitrogen 0.10%, and alkali hydrolyzed nitrogen 130mg·kg -1 , Available phosphorus 114mg·kg -1 、Quick-acting potassium 284mg·kg -1 , With a pH of 6.08, it is a medium loam soil. After being air-dried and ground by a soil grinder, it is passed through a 100-mesh sieve to obtain basic soil. Set 5 concentration gradients of DBP and fenvalerate, respectively 0, 25, 50, 75, 100 mg·kg -1 , Added to the basic soil to obtain artificial poisonous soil. Each artificially-contaminated soil sample was 40g, set in 3 parallel, put into a clean glass petri dish, and all samples were sterilized by autoclaving at 121℃ twice a day for 20 minutes each time. After the strain wa...

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Abstract

The invention discloses a fungus capable of degrading phthalic acid ester and pesticide simultaneously and application thereof. The name of the fungus is Geotrichum sp.DY4, and the fungus is preserved in China Center for Type Culture Collection in Wuhan University in Wuhan, China on May 11, 2014 with the preservation number of CCTCC NO:M2014194. The fungus disclosed by the invention can degrade phthalic acid ester and pesticide simultaneously, can be used for degrading pollution caused by multiple PAEs and pesticide mixtures and also can be used for soil biological remediation, and a new genetic resource is provided for study of a degradation mechanism on PAEs and pesticide in soil.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, in particular to a fungus capable of simultaneously degrading phthalates and pesticides and its application. Background technique [0002] In order to meet the growing demand for various materials, human beings use a large amount of agricultural chemicals such as mulch film, organic pesticides, and chemical fertilizers in agricultural production, resulting in the compound pollution of various organic substances to the soil. The mulching film in the farmland soil has the effect of heat preservation and moisture retention for crops, especially in the process of growing vegetables and fruits in greenhouses. It plays a very important role. However, the plasticizer in the mulch - phthalic acid esters (PAEs) is difficult to firmly bond with the plastic itself, and its stability is relatively poor. PAEs are easy to seep out of the plastic mulch, causing s...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C12R1/645
Inventor 蔡信德王冬莹吴文成方晓航李诗殷
Owner SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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