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Method for dissecting adult frankliniella occidentalis

A technology for Western flower thrips and adults, which is applied in the field of anatomy of Western flower thrips adults, and can solve the problems of high temperature resistance, drug-resistant population proliferation, no anatomical method for Western flower thrips, agricultural disasters of Western flower thrips, and the like, achieve the effect of guaranteed integrity

Inactive Publication Date: 2014-11-19
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the warming of the global temperature and the abuse of pesticides, it has led to the flooding of high-temperature resistant and drug-resistant populations, and the flooding of western flower thrips has brought about agricultural disasters. It needs to be studied. At present, there is no effective western flower thrips How to dissect flower thrips

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0041] Implementation case 1: An anatomical method for adults of Thrips occidentalis for digestive system analysis.

[0042] Put the adult thrips western flower into a petri dish filled with PBS buffer solution and soak for 4 to 5 minutes; use a fine brush to fix the worm body in the groove of the concave glass slide filled with a thin layer of buffer solution, with the ventral surface of the worm body upward; Use a dissecting needle to pick apart the intersegmental membranes at the end of the abdomen; press the head with tweezers with the left hand, and gently pull back the end of the abdomen with the other tweezers with the right hand, and use a fine needle to absorb buffer to rinse the pulled out organs. The digestive tract was then isolated with a dissecting needle, and the structure was observed under an 800X Leica microscope.

Embodiment example 2

[0043] Implementation case 2: An anatomical method for adults of Thrips occidentalis for reproductive system analysis.

[0044] Put the adult thrips western flower into a petri dish filled with PBS buffer solution and soak for 4 to 5 minutes; use a fine brush to fix the worm body in the groove of the concave glass slide filled with a thin layer of buffer solution, with the ventral surface of the worm body upward; Use a dissecting needle to pick apart the intersegmental membranes at the end of the abdomen; press the head with tweezers with the left hand, and gently pull back the end of the abdomen with the other tweezers with the right hand, and use a fine needle to absorb buffer to rinse the pulled out organs. Then the ovary was isolated with a dissecting needle, and the structure of the ovary was observed under a Leica microscope at 800 times.

Embodiment example 3

[0045] Example 3: An anatomical method for adults of Thrips occidentalis for the analysis of ovarian development process.

[0046] Put the adult worms of different developmental stages of Thrips occidentalis into a petri dish filled with PBS buffer solution and soak for 4 to 5 minutes; Use a dissecting needle to pick apart the intersegmental membranes at the end of the abdomen; press the head with tweezers with your left hand, and gently pull back at the end of the abdomen with the other tweezers with your right hand, and use a fine needle to draw buffer to rinse the pull Then the ovary was separated with a dissecting needle, and the structure of the ovary at different developmental times was observed under a Leica microscope at 800 magnifications to determine the developmental process.

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PUM

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Abstract

The invention relates to a method for dissecting adult frankliniella occidentalis. The method comprises the following steps that firstly, adult frankliniella occidentalis is collected and immersed in a culture dish containing a PBS buffer solution in a short time and stands, and the frankliniella occidentalis body at the bottom is collected; secondly, the frankliniella occidentalis body is fixed to a concave slide groove containing a thin layer of buffer solution through a fine writing brush, the segmental venter of the frankliniella occidentalis body faces upwards, and the frankliniella occidentalis body is placed below an anatomical lens to be dissected; thirdly, several sections of segmacorias at the tail end of the segmental venter are picked up through a dissecting needle; fourthly, a worker presses the head of the frankliniella occidentalis body with the left hand through a nipper, clamps the tail end of the abdomen of the frankliniella occidentalis body with the right hand through another nipper and slightly pulls backwards, and then the whole reproductive organ and the digestive organs can be completely exposed. By means of the dissecting method, the internal structures of the adult frankliniella occidentalis can be obtained completely, and people can understand the physiological structure of the internal organs of rasping-mouthpart insects better; people can observe the ovary structures at different times and can understand the growing process of the ovaries, and a theoretical basis is provided for predicting of the frankliniella occidentalis.

Description

technical field [0001] The invention relates to the field of insect anatomy, in particular to a method for dissecting adults of western flower thrips. Background technique [0002] Western flower thrips Frankliniella occidentalis (Pergande) belongs to Thysanoptera, Thripidae, and the genus Flower Thrips. Western flower thrips has a wide range of hosts. At present, there are more than 500 kinds of plants in 60 families, including important leguminous, cruciferous, cucurbitaceous and other vegetables, and composites, impatiens, Rosaceae and other flowers. , with the spread of Thrips occidentalis, its host species will continue to increase. [0003] Western flower thrips was first recorded in 1895. It originated from the Rocky Mountains in the western part of the United States and Canada. After the 1980s, with the rapid development of international trade, western flower thrips quickly spread to Spread out and become a worldwide agricultural pest. It has been widely distribut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B16/00
Inventor 郑长英马亚斌张彬史光华孙丽娟王思芳李洪刚张瑜冯志国顾松东郑伯平张乃芹王俊平
Owner QINGDAO AGRI UNIV
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