RT-PCR kit for identification and diagnosis of porcine epidemic diarrhea virus and application of RT-PCR kit
A porcine epidemic diarrhea and RT-PCR technology, which is applied in the field of porcine epidemic diarrhea virus RT-PCR differential diagnosis kits, can solve the problems of inability to realize the differential diagnosis of natural infection and vaccine immune strains, and achieves high sensitivity and specificity. strong effect
Active Publication Date: 2014-11-19
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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The invention discloses a RT-PCR kit for identification and diagnosis of a porcine epidemic diarrhea virus (PEDV). The kit comprises a pair of primers designed for deleted regions of nucleotide sequences at positions of 245-294 of an ORF3 gene of an attenuated vaccine strain of the PEDV, wherein the forward and reverse primers of the pair of primers are respectively located in conserved regions at both ends of the deleted regions of the ORF3 gene. The RT-PCR kit for identification and diagnosis of the PEDV based on the ORF3 gene disclosed by the invention has the characteristics of strong specificity and high sensitivity. By virtue of the RT-PCR kit, whether the PEDV is present in diarrhea samples of a piglet suffering from diarrhea or not can be rapidly detected and a naturally infected wild strain can be also rapidly and accurately distinguished from the attenuated vaccine strain. The RT-PCR kit is of important significance for rapid diagnosis, prevention and control of early stage of porcine epidemic diarrhea.
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