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A detection kit for the concentration of tnni3k in blood and its preparation method and application

A technology for detecting kits and concentrations, applied in measuring devices, analyzing materials through chemical reactions, and analyzing materials through observing the impact on chemical indicators, can solve the problems of long detection time and multiple blood samples, and achieve Short time consumption and improved sensitivity

Inactive Publication Date: 2016-04-27
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the present invention aims to solve the above-mentioned technical problems that the detection of TNNI3K concentration in blood takes a long time, requires more blood samples and special instruments, etc., and provides a detection time-consuming short, requires a small amount of blood samples, and does not require special instruments. Detection kit for the concentration of TNNI3K in the blood of the instrument

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  • A detection kit for the concentration of tnni3k in blood and its preparation method and application
  • A detection kit for the concentration of tnni3k in blood and its preparation method and application
  • A detection kit for the concentration of tnni3k in blood and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] A detection kit for the concentration of TNNI3K in blood, comprising:

[0066] (1) Aqueous solution of mouse anti-human TNNI3K monoclonal antibody at a concentration of 0.01 μg / mL;

[0067] (2) Goat anti-mouse IgG (H+L) antibody aqueous solution with a concentration of 0.1 μg / mL;

[0068] (3) Neodymium nitrate solution with a neodymium concentration of 1 mg / mL;

[0069] (4) Mixture of nano-silver-nano-neodymium-goat anti-mouse IgG(H+L) antibody

[0070] In the mixed solution of described nano-silver-nano-neodymium-sheep anti-mouse IgG (H+L) antibody, calculated by mass ratio, that is, nano-silver: nano-neodymium: sheep anti-mouse IgG (H+L) antibody is 6.4:1 :0.2;

[0071] (4), glycerol PBS buffer

[0072] The PBS buffer solution of glycerol is obtained by adding glycerin to a disodium hydrogen phosphate aqueous solution with a pH value of 7.4 and mixing uniformly, wherein the volume percentage concentration of glycerol is 30%;

[0073] (5), the carrier with the sma...

Embodiment 2

[0117] A detection kit for the concentration of TNNI3K in blood, comprising:

[0118] (1) Mouse anti-human TNNI3K monoclonal antibody aqueous solution with a concentration of 0.3 μg / mL;

[0119] (2) Goat anti-mouse IgG (H+L) antibody aqueous solution with a concentration of 2 μg / mL;

[0120] (3) Yttrium chloride solution with an yttrium concentration of 5 mg / mL;

[0121] (4) Mixture of nano-silver-nano-yttrium-goat anti-mouse IgG(H+L) antibody

[0122] In the mixed solution of described nanometer silver-nanometer yttrium-sheep anti-mouse IgG (H+L) antibody, calculate by mass ratio, namely nanometer silver: nanometer yttrium: sheep anti-mouse IgG (H+L) antibody is 1.2:1.6 :0.24;

[0123] (5), glycerol PBS buffer

[0124] The PBS buffer solution of glycerol is obtained by adding glycerin to a disodium hydrogen phosphate aqueous solution with a pH value of 7.4 and mixing uniformly, wherein the volume percentage concentration of glycerol is 30%;

[0125] (6), the carrier with...

Embodiment 3

[0164] A detection kit for the concentration of TNNI3K in blood, comprising:

[0165] (1) Aqueous solution of mouse anti-human TNNI3K monoclonal antibody at a concentration of 0.15 μg / mL;

[0166] (2) Goat anti-mouse IgG (H+L) antibody aqueous solution with a concentration of 1 μg / mL;

[0167] (3) Samarium nitrate solution with a samarium concentration of 2.5 mg / mL and erbium nitrate solution with an erbium concentration of 2 mg / mL;

[0168] (4) Mixture of nano-silver-nano-samarium and erbium-goat anti-mouse IgG (H+L) antibody

[0169]In the mixed solution of described nanometer silver-nano samarium and erbium-sheep anti-mouse IgG (H+L) antibody, calculate by mass ratio, namely nanometer silver: nanometer samarium and erbium: sheep anti-mouse IgG (H+L) antibody is 3.8:1.34:0.22;

[0170] (4), glycerol PBS buffer

[0171] The PBS buffer solution of glycerol is obtained by adding glycerin to a disodium hydrogen phosphate aqueous solution with a pH value of 7.4 and mixing uni...

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Abstract

The invention discloses a test kit for detecting the concentration of TNNI3K in blood and its preparation method and application. The test kit for detecting the concentration of TNNI3K in blood includes a mouse anti-human TNNI3K monoclonal antibody with a concentration of 0.01-0.3 μg / mL Aqueous solution, goat anti-mouse IgG (H+L) antibody aqueous solution with a concentration of 0.1-2 μg / mL, rare earth salt solution with a concentration of 1-5 mg / mL, nano-silver-nano-rare earth-goat anti-mouse IgG (H+L) antibody , glycerol in PBS buffer, a carrier with small circles with a depth and diameter of 0.5 mm and 6 mm, respectively. The detection kit for the concentration of TNNI3K in blood can be used to detect the concentration of TNNI3K in blood, can detect 0.008ng TNNI3K / point, and consumes less time and less sample.

Description

technical field [0001] The invention relates to a detection kit for the concentration of TNNI3K in blood, a preparation method and application thereof. Background technique [0002] Ischemic heart disease is mostly caused by obstruction or narrowing of coronary arteries, including atherosclerotic lesions. Since ischemic heart disease is characterized by sudden onset and high mortality, early diagnosis and corresponding treatment within hours to 24 hours after the onset of onset and within 3 days to a week are the main factors that determine its life-saving rate. Generally speaking, although the diagnosis can be made based on the ischemic elevation of the S-T segment of the electrocardiogram and the elevation of CPK, cardiac troponin I, T (cTnI, cTnT), myoglobin (MB) and other indicators, there are still many cases that are difficult to diagnose. Diagnose quickly. [0003] In particular, although the determination of serum myoglobin (MB) can be used as the most sensitive in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/544G01N21/78
CPCG01N33/532G01N33/577G01N33/6836
Inventor 刘小珍任晓辉刘小舟陈捷
Owner SHANGHAI INST OF TECH