Efficient breeding method suitable for limuli from germ cells to 2-age phase

A fertilized egg, high-efficiency technology, applied in the field of high-efficiency breeding, can solve the problems of cumbersome hatching process and low cumulative survival rate of horseshoe crabs, and achieve the effects of simple and easy larvae cultivation, high hatching density, and high embryo breeding efficiency

Inactive Publication Date: 2014-12-03
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to change the traditional cumbersome incubation process of horseshoe crabs and the low cumulative survival rate of cultivation before the 2nd age, improve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Artificial breeding process from fertilized eggs to 2-year-old horseshoe crabs under indoor temperature control

[0029] A kind of high-efficiency breeding method applicable to horseshoe crab from fertilized egg to 2 instar stage of the present invention, it comprises the following steps:

[0030] a. Assembly of simple hatching equipment: In the first ten days of June 2010, a 500L glass steel bucket was used as a hatching place for fertilized eggs, and fresh secondary sand was added to filter seawater. Utilize 12 apertures afterwards to be 2mm, diameter be 40cm, the circular plastic basket that depth is 16cm as hatching basket, place 12 hatching baskets in each fiberglass bucket hatching place. Tape foam around the hatcher baskets to keep the unit afloat, resting on the surface of the seawater. Among them, the temperature of seawater is 29-32°C, the salinity is 27-30ppt, the pH is 7.8-8.5, and D0≥6mg / L.

[0031] b. Incubation of fertilized eggs: 5cm-6cm thi...

Embodiment 2

[0033] Embodiment 2, the process of artificial breeding from fertilized egg to 2-year-old horseshoe crab at outdoor normal temperature

[0034] A kind of high-efficiency breeding method applicable to horseshoe crab from fertilized egg to 2 instar stage of the present invention, it comprises the following steps:

[0035]a. Simple hatching equipment assembly: In the first ten days of August 2012, three 500L glass steel barrels were used as fertilized egg hatching places, and fresh seawater was added to the secondary sand filter. Utilize 36 apertures to be 2mm afterwards, be 40cm in diameter, the circular plastic basket that depth is 16cm is used as hatching container, and 12 hatching baskets are placed in each glass-steel barrel hatching place. Tape foam around the hatcher baskets to keep the unit afloat, resting on the surface of the seawater. Among them, the temperature of seawater is 28-32°C, the salinity is 25-30ppt, the pH is 7.6-8.5, and DO≥6mg / L.

[0036] b. Incubation ...

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Abstract

The invention discloses an efficient breeding method suitable for limuli from germ cells to a 2-age phase. The efficient breeding method includes the following steps of a, assembling a simple hatching device: using glass steel buckets as a germ cell hatching place and using plastic baskets as hatching baskets, wherein the hatching baskets stay on the surface of water, and sufficient dissolved oxygen (DO) exists; b, hatching germ cells: placing a plurality of the hatching baskets into each glass steel bucket, placing 3000 to 8000 germ cells with the thickness of 3cm to 6cm into each hatching basket, hatching 70 thousand to 150 thousand germ cells in each glass steel bucket, and subjecting the hatching baskets to microaeration, wherein conditions that the water temperature is 28 to 32 DEG C, the salt degree is 20 to 32 ppt, pH is 7.6 to 8.5, and DO>=6mg/L are guaranteed, and germ cells break off after 36 to 45 days of embryonic development to become 1-age limulus larvae; c, breeding 1-age to 2-age limuli; d, stocking 2-age limuli, wherein after 2 ages, limulus larvae begin to feed and are subjected to microaeration stocking in a sanded environment at a density of 900 to 1200 per square meter. Compared with traditional limulus breeding methods, the efficient breeding method has the advantages that breeding efficiency is increased by 15-30 times, and the efficient breeding method is suitable for large-scale limulus artificial breeding.

Description

technical field [0001] The invention relates to the technical field of modern rare animal breed breeding, in particular to a high-efficiency breeding method suitable for horseshoe crabs from fertilized eggs to the second instar stage. Background technique [0002] Horseshoe crabs belong to the phylum Arthropoda, the subphylum Chelicidae, the class Limostomia, the order Xyphobia, and the family Limulidae. Existing species of horseshoe crabs are few, and their distribution is narrow. According to their shape and distribution characteristics, they can be divided into two subfamilies, three genera and four types. Among them, the Limulinae subfamily has only one species of Limulus and L polyphemus, which are distributed in the east coast waters of North America; and the Tachypleinae subfamily has two genera, which are distributed in the southeast coast and east coast waters of Asia. Among them, Tachypleus has two species: T. tridentatus and T. gigas; Carcinoscorpius has only one...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 吴铁军甘晖罗帮农钊陈秀荔龚竹林张跃环蓝嘉
Owner GUANGXI ACADEMY OF FISHERY SCI
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