Efficient breeding method suitable for limuli from germ cells to 2-age phase
A fertilized egg, high-efficiency technology, applied in the field of high-efficiency breeding, can solve the problems of cumbersome hatching process and low cumulative survival rate of horseshoe crabs, and achieve the effects of simple and easy larvae cultivation, high hatching density, and high embryo breeding efficiency
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Embodiment 1
[0028] Example 1: Artificial breeding process from fertilized eggs to 2-year-old horseshoe crabs under indoor temperature control
[0029] A kind of high-efficiency breeding method applicable to horseshoe crab from fertilized egg to 2 instar stage of the present invention, it comprises the following steps:
[0030] a. Assembly of simple hatching equipment: In the first ten days of June 2010, a 500L glass steel bucket was used as a hatching place for fertilized eggs, and fresh secondary sand was added to filter seawater. Utilize 12 apertures afterwards to be 2mm, diameter be 40cm, the circular plastic basket that depth is 16cm as hatching basket, place 12 hatching baskets in each fiberglass bucket hatching place. Tape foam around the hatcher baskets to keep the unit afloat, resting on the surface of the seawater. Among them, the temperature of seawater is 29-32°C, the salinity is 27-30ppt, the pH is 7.8-8.5, and D0≥6mg / L.
[0031] b. Incubation of fertilized eggs: 5cm-6cm thi...
Embodiment 2
[0033] Embodiment 2, the process of artificial breeding from fertilized egg to 2-year-old horseshoe crab at outdoor normal temperature
[0034] A kind of high-efficiency breeding method applicable to horseshoe crab from fertilized egg to 2 instar stage of the present invention, it comprises the following steps:
[0035]a. Simple hatching equipment assembly: In the first ten days of August 2012, three 500L glass steel barrels were used as fertilized egg hatching places, and fresh seawater was added to the secondary sand filter. Utilize 36 apertures to be 2mm afterwards, be 40cm in diameter, the circular plastic basket that depth is 16cm is used as hatching container, and 12 hatching baskets are placed in each glass-steel barrel hatching place. Tape foam around the hatcher baskets to keep the unit afloat, resting on the surface of the seawater. Among them, the temperature of seawater is 28-32°C, the salinity is 25-30ppt, the pH is 7.6-8.5, and DO≥6mg / L.
[0036] b. Incubation ...
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