A kind of polypeptide and its application in the preparation of voltage-sensitive sodium channel inhibitor

A sodium channel inhibitor, voltage-sensitive technology, applied in the biological field, can solve the problems of long cycle of natural polypeptides

Inactive Publication Date: 2016-12-28
NAT UNIV OF DEFENSE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the peptide drugs (inhibitors) targeting voltage-sensitive sodium channels sold on the market are all developed and designed from naturally occurring peptides. However, it is a long process from the discovery of natural peptides to the preparation of drugs.

Method used

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  • A kind of polypeptide and its application in the preparation of voltage-sensitive sodium channel inhibitor
  • A kind of polypeptide and its application in the preparation of voltage-sensitive sodium channel inhibitor
  • A kind of polypeptide and its application in the preparation of voltage-sensitive sodium channel inhibitor

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Experimental program
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Embodiment Construction

[0014] 1. Yeast two-hybrid method to obtain positive clones, and self-activation verification

[0015] The yeast two-hybrid experiment was carried out using the MATCHMAKER GAL4Two-Hybrid System3 system of clontech company. The specific steps are: 1) will contain hNa v The S3-S4 extracellular linking loop of 1.5 channel Domain II (hNa v 1. The bait plasmid (BD) of 5-DII-S3-S4) was transformed into yeast strain AH109, ​​and it was prepared to be competent by PEG / LiAc method; Competent; 3) Finally, spread the competent cells on the growth-defective medium and culture at 30°C until the positive clones grow; 4) Small-scale expansion culture of the positive clones, extraction of plasmids, transfer into AH109, ​​and smear on Repeat the experiment and self-activation verification on a suitable defective medium; 5) Send the positive plasmid without self-activation to the sequencing company for sequencing.

[0016] Through the above steps, we found that 3 library plasmids can interac...

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Abstract

The invention discloses a polypeptide. The sequence of the polypeptide is shown as SEQ ID NO.1. The polypeptide can be used for inhibiting voltage-sensitive sodium channels. When the voltage-sensitive sodium channels are inhibited by the polypeptide, the concentration of the polypeptide is 10-50 [mu]M, preferably 10 [mu]M. The polypeptide can also be used for preparation of a voltage-sensitive sodium channel inhibitor.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a polypeptide and its application in the preparation of voltage-sensitive sodium channel inhibitors. Background technique [0002] Voltage-sensitive sodium channels are an important part of the rise and transmission of nerve cell action potentials, and are inseparable from the human body's movement coordination and injury perception (pain). Some common and incurable diseases, such as gout, hyperalgesia, Parkinson's disease, Huntington's disease, etc., are all related to the abnormal opening of voltage-sensitive sodium channels, so voltage-sensitive sodium channels are one of the targets for drug development. However, the peptide drugs (inhibitors) targeting voltage-sensitive sodium channels on the market are all developed and designed from naturally occurring peptides. However, it is a long process from the discovery of natural peptides to the formulation of drugs. Theref...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00A61K38/16
CPCA61K38/00C07K14/00
Inventor 孟尔张东裔李文颖唐锶彭宽蒋辉张辉吴磊柳珑朱凌云
Owner NAT UNIV OF DEFENSE TECH
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