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Application of apigenin to preparation of medicines for inhibiting scaffolding protein expression and cancer cell metastasis

A technology of scaffold protein and apigenin, which is applied in the field of medicine to achieve the effects of convenient use, inhibition of migration, and less toxic and side effects

Inactive Publication Date: 2014-12-10
NANJING YIDU MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there is no report about apigenin inhibiting the expression of the scaffold protein HEF1, thereby inhibiting the metastasis of cancer cells. Therefore, the present invention applies apigenin for the first time to inhibit the expression of HEF1 in colon cancer cells, thereby inhibiting the migration and invasion of cancer cells, indicating that its Has good prospects for development and utilization

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  • Application of apigenin to preparation of medicines for inhibiting scaffolding protein expression and cancer cell metastasis
  • Application of apigenin to preparation of medicines for inhibiting scaffolding protein expression and cancer cell metastasis
  • Application of apigenin to preparation of medicines for inhibiting scaffolding protein expression and cancer cell metastasis

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Embodiment Construction

[0026] The purpose, function and technical means of the present invention are further understood. Now the present invention is explained in detail in conjunction with the following experiments. The migration and invasion of cancer cells are important steps of cancer cell metastasis. Migration and invasion were assessed by diluting apigenin to 30-200 uM (concentration used in cells) in 5% DMSO in saline solution, hereinafter referred to as apigenin.

[0027] 1. Determine the concentration of apigenin by MTT experiment:

[0028] Colon cancer cells DLD1 and SW480 were selected in this experiment.

[0029] Experimental steps:

[0030] 1) Collect log-phase cells and add 100uL log-phase cells at an appropriate concentration; when the cell monolayer is cultured to cover the bottom of the well, add apigenin with a concentration gradient, and then add the drug after the cells adhere to the wall, and set 4 gradients , 100uL apigenin per well, and set up 4 replicate wells;

[0031] 2)...

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Abstract

The invention discloses application of an apigenin preparation to preparation of medicines for inhibiting expression of scaffolding protein HEF1 to inhibit cancer cell metastasis. Experiments prove that the apigenin preparation is capable of degrading either endogenous HEF1 molecules or HEF1 molecules generated by artificial overexpression. In conclusion, the invention discloses a fact that the apigenin is capable of inhibiting expression of HEF1 and further inhibiting metastasis and invasion of cancer cells.

Description

【Technical field】 [0001] The invention belongs to the field of medicine, and relates to the application of apigenin in the preparation of a medicine for inhibiting a scaffold protein so as to inhibit the metastasis of cancer cells. 【Background technique】 [0002] Apigenin mainly exists in Periaceae, Verbena, and Selaginaceae plants, such as coriander, and Selaginella has a large content. Apigenin belongs to the class of flavonoids and has the activity of inhibiting carcinogens; as an antiviral drug for the treatment of HIV and other viral infections; an inhibitor of MAP kinase; treatment of various inflammations; antioxidant; sedative, soothing; blood pressure. Compared with other flavonoids (quercetin, kaempferol), it has the characteristics of low toxicity and no mutagenicity. [0003] Metastasis is one of the main reasons for the high mortality of cancer, and the research on anti-metastatic drugs is an important direction for the development of anti-tumor drugs. Recentl...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P35/04
Inventor 陆健张竹易周中平
Owner NANJING YIDU MEDICAL TECH CO LTD