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Method for identifying iron deficiency-resistant apple rootstocks

A technology for iron-deficiency and apples, applied in the field of identification of iron-deficiency-resistant apple rootstocks, can solve problems such as complex genetic background, high gene heterozygosity, and difficulty in application, and achieve the effects of overcoming limitations, low cost, and high feasibility

Active Publication Date: 2014-12-10
CHINA AGRI UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

Apples are mostly perennial woody plants with highly heterozygous genes and complex genetic backgrounds, so traditional breeding methods are still difficult to apply to fruit trees

Method used

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  • Method for identifying iron deficiency-resistant apple rootstocks
  • Method for identifying iron deficiency-resistant apple rootstocks
  • Method for identifying iron deficiency-resistant apple rootstocks

Examples

Experimental program
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Effect test

Embodiment 1

[0061] Embodiment 1, adopt Dot-blot-macroarray method to identify iron-deficiency-resistant apple stock

[0062] 1. Obtaining apple rootstocks to be tested and iron deficiency treatment

[0063] The 20-year-old Malus xiaojinensis Cheng et Jiang of the iron-efficient genotype was used as the female parent tree, and the iron-inefficient genotype Malus baccata Bokh. was used as the male parent tree. Sowed in 2010, received a total of 145 F 1 Group, 2012 cutting propagation, each line selects three cutting seedlings with the same growth, and transplants them in the river sand matrix cultivation tank. The iron ion concentration is 40μmol·L during normal iron supply -1 , the concentration of iron ions was 4 μmol L in iron deficiency treatment -1 . Specifically, the improved Hoagland’s nutrient solution was provided by drip irrigation to the river sand matrix that was completely rinsed with deionized water and tested to contain no iron ions, and the iron ion concentration was 40 ...

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Abstract

The invention discloses a method for identifying iron deficiency-resistant apple rootstocks. The method comprises the following steps: 1) conducting iron deficiency treatment on an apple rootstock to be detected and a standard iron deficiency-resistant apple rootstock respectively, and extracting total RNA; 2) conducting dot-blot hybridization on the total RNA with an iron deficiency-resistant gene probe; 3) scanning with an image scanner, and conducting an analysis with an image master to obtain the expression quantities of the iron deficiency-resistant genes in the apple rootstock to be detected and the standard iron deficiency-resistant apple rootstock; and 4) if the expression quantity of the iron deficiency-resistant genes in the apple rootstock to be detected is not lower than that of the iron deficiency-resistant genes in the standard iron deficiency-resistant apple rootstock, determining that the apple rootstock to be detected is an iron deficiency-resistant apple rootstock or an iron deficiency-resistant apple rootstock candidate. The method provided by the invention has the advantages of low cost and high efficiency, overcomes the limitation of conventional selective breeding of iron deficiency-resistant apple rootstocks, conducts selective breeding at the gene level, and lays a good foundation for selective breeding with the aid of gene expression marking.

Description

technical field [0001] The invention relates to a method for identifying iron-deficiency-resistant apple rootstocks. Background technique [0002] Iron is an essential nutrient element for plant growth and development, and plays an extremely important role in many physiological metabolic processes such as photosynthesis, respiration, nitrogen fixation, and protein and nucleic acid synthesis in plants. More than 40% of the world's soil is iron-deficient, and iron-deficiency chlorosis has become one of the key problems of plant nutritional imbalance. In order to adapt to iron deficiency stress and absorb more iron from the soil to meet the needs of their own growth and development, higher plants have gradually formed different adaptive mechanisms during the long-term evolution process. Romheld and Marschner, on the basis of summarizing previous research work and the research on the response mechanism of iron deficiency in 120 plants from different sources, first proposed two ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13
Inventor 韩振海朱明涛张新忠王忆吴婷贾文锁许雪峰
Owner CHINA AGRI UNIV
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