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Vaccine composition, and preparation method and application thereof

A technology of vaccine composition and bronchi, applied in the field of vaccine composition, can solve the problems of no obvious clinical symptoms, no detection of Bordetella bronchiseptica pathogen, etc., to increase the scope of application, good immune effect, reduce The effect of the stress response

Active Publication Date: 2014-12-17
PU LIKE BIO ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In the artificial reproduction of the mixed infection test of porcine atrophic rhinitis in the present invention, the inventor unexpectedly found that the pig body was infected with a small amount of Bordetella bronchiseptica, without obvious clinical symptoms, and even bronchial septicemia was not detected. Bordetella pathogens exist, but when pigs are mixed with toxin-producing Pasteurella multocida (Pm) type A or D, it will produce severe symptoms of porcine wasting disease rhinitis, which is more than a single infection with toxin-producing Pasteurella. Significantly severe symptoms when Pasteurella genus (Pm) type A or D

Method used

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  • Vaccine composition, and preparation method and application thereof
  • Vaccine composition, and preparation method and application thereof
  • Vaccine composition, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation of the vaccine composition of embodiment 1 treatment and prevention mixed infection porcine atrophic rhinitis

[0044] 1. Preparation of bacterial solution

[0045] Streak the Bordetella bronchiseptica HN8 strain on the modified Bauer Jiang's blood plate, culture at 37°C for 48 hours, pick the single clone with obvious hemolytic ring in a test tube containing 5ml BHI, and shake at 150-220rpm, 37°C After 16-24 hours, the bacterial solution was expanded and cultivated at 37°C for 12-16 hours with a 1% inoculation amount, and the rotation speed was 150-220rpm. Samples were taken for a series of ten-fold dilutions, and counted on a plate.

[0046] Streak the strain of Pasteurella type D HB4 strain on the TSA plate, culture at 37°C for 16-20h, pick a single clone in a test tube containing 5ml of BHI, shake at 150-220rpm, 37°C for 16-24h, then The bacterial solution was expanded and cultivated at 37°C for 12-16 hours with a 1% inoculum size, and the rotation...

Embodiment 2

[0051] Example 2 Preparation of vaccine composition for treating and preventing mixed infection porcine atrophic rhinitis

[0052] The inactivation of bacterial classification and bacterial classification, the extraction of PMT toxoid and inactivation are the same as embodiment 1.

[0053] See Table 1 below for the ratio of each antigen in Example 2.

Embodiment 3

[0054] Example 3 Preparation of vaccine composition for treating and preventing mixed infection porcine atrophic rhinitis

[0055] The inactivation of bacterial classification and bacterial classification, the extraction of PMT toxoid and inactivation are the same as embodiment 1.

[0056] See Table 1 below for the ratio of each antigen in Example 3.

[0057] The concrete proportioning ratio of the vaccine of embodiment 1-3 is shown in Table 1:

[0058] The composition ratio of the vaccine composition of table 1 porcine atrophic rhinitis

[0059]

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Abstract

The invention provides a vaccine composition, and the vaccine composition comprises immunization amount of a bordetella bronchiseptica antigen, immunization amount of pasteurella multocida toxoid antigen and an adjuvant acceptable in veterinary medicine. The vaccine composition is capable of effectively controlling co-infected swine atrophic rhinitis caused by bordetella bronchiseptica and pasteurella multocida, and further comprises other antigen compositions for controlling respiratory syndrome caused by secondary infection of swine atrophic rhinitis. By reducing the antigen composition and using pasteurella multocida toxoid to replace pasteurella multocida antigens with different serotypes, the purpose of preventing swine atrophic rhinitis is realized. Also by improving the antigen content in per volume of the vaccine composition, the immunization dosage of the vaccine composition is reduced to 1 / 4 of the dosage of a conventional vaccine without reducing the immunization effect. Therefore, the vaccine composition is relatively low in immunization dosage, relatively less in antigen compositions, relatively low in immunization cost and convenient to use.

Description

technical field [0001] The invention relates to a vaccine composition for preventing and treating mixed-infected porcine atrophic rhinitis, in particular to a water-based adjuvant inactivated vaccine for porcine atrophic rhinitis with less antigenic components and a small immune dose. Background technique [0002] Atrophic rhinitis (AR) is an important infectious disease of the respiratory system in pigs. It can cause symptoms such as chronic rhinitis, sneezing, tearing, facial degeneration and turbinate atrophy in pigs. It can cause snout deformation and affect the bone development of the whole body, thereby causing growth retardation of pigs, making pigs susceptible to secondary complex pneumonia, and causing huge losses to the pig industry. The disease can infect pigs of any age, among which the infection rate of pigs within 4 weeks of age is the highest, and symptoms generally appear at 8-12 weeks of age. [0003] Porcine atrophic rhinitis can be divided into progressiv...

Claims

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Application Information

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IPC IPC(8): A61K39/102A61K38/16C07K14/00A61P11/02A61P11/00A61P31/04A61K39/10
Inventor 张许科孙进忠田克恭
Owner PU LIKE BIO ENG
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