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A live attenuated vaccine of fish gill rot pathogen Flavobacter johnii and its construction method

A technology of Flavobacterium johniii live attenuated vaccine, which is applied in the field of fish gill rot pathogen Flavobacterium johniii live attenuated vaccine and its construction, which can solve the problems of dampening fishermen's production enthusiasm, wide range of disease, and long epidemic time. , to achieve good application value, broad market prospects, and the effect of small immune dose

Active Publication Date: 2017-12-12
湖北宜水生物科技有限公司
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Problems solved by technology

Among them, bacterial gill rot caused by Flavobacterium is one of the most common diseases in aquaculture. Serious economic losses have hit fishermen's production enthusiasm, brought about quality and safety problems of aquatic products such as drug residues, and restricted the sustainable and healthy development of my country's aquaculture industry
[0003] At present, chemical drugs such as disinfectants and antibiotics are mainly used to treat fish bacterial gill rot in China. However, the extensive use of chemical drugs has led to the emergence of drug-resistant strains, which has a negative impact on the quality and safety of aquatic products and ecological security.

Method used

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  • A live attenuated vaccine of fish gill rot pathogen Flavobacter johnii and its construction method
  • A live attenuated vaccine of fish gill rot pathogen Flavobacter johnii and its construction method
  • A live attenuated vaccine of fish gill rot pathogen Flavobacter johnii and its construction method

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Experimental program
Comparison scheme
Effect test

Embodiment

[0022] 1. Induction of attenuated mutant strains of Flavobacter johniiii

[0023] The pathogenic Flavobacterium johnii strain (M168) was isolated from freshwater fish suffering from gill rot, and after purification and cultivation by streaking on a plate, the concentration of the bacterial solution was adjusted to 10 with sterilized isotonic saline. 7 CFU / mL spare. Add 0.005, 0.01, 0.02, 0.03, 0.06, 0.13, 0.25, 0.5, 1, 2, 4, 8, 16, 32, 64, 128 μg / mL streptomycin to the M-H broth medium, and prepare different medium with streptomycin concentration. Inoculate 10 μL of the above-prepared bacterial suspension into 2 mL broth medium containing 0.005 μg / mL streptomycin, incubate at 28°C for 24 h, and if there is bacterial growth, transfer it to a medium containing 0.01 μg / mL streptomycin If bacteria grow after incubation at 28°C for 24 hours, transfer to the next concentration of streptomycin medium. If no bacterial growth is found, continue to culture in the previous concentratio...

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Abstract

The invention discloses a fish gill rot pathogen Flavobacter johnii live attenuated vaccine and a construction method. In the present invention, the wild-type Flavobacterium johniiii is continuously subcultured by streptomycin to induce mutations to weaken its virulence, and the attenuated mutant strain M170 of Flavobacterium johniiii is obtained, and the live vaccine prepared by using the attenuated strain is injected and soaked to immunize grass carp, The immune protection rate of mandarin fish and others can reach more than 73.1%, and the immune protection period can reach more than 8 months. It has the advantages of small immune dose, high potency, high protection rate, and long protection period. Preventing the occurrence of bacterial gill rot diseases such as grass carp and mandarin fish has good application value and broad market prospects.

Description

technical field [0001] The invention relates to a fish gill rot pathogen Flavobacter johnii ( Flavobacterium johnsoniae ) live attenuated vaccine and its construction method. Background technique [0002] China is a big country in aquaculture, and its aquaculture production accounts for 70% of the world's total production. With the rapid development of my country's aquaculture industry, the expansion of aquaculture scale and the increase of unit output, the problem of disease has become increasingly prominent. Among them, bacterial gill rot caused by Flavobacterium is one of the most common diseases in aquaculture. Serious economic losses have hit fishermen's production enthusiasm, brought about quality and safety problems of aquatic products such as drug residues, and restricted the sustainable and healthy development of my country's aquaculture industry. [0003] At present, chemical drugs such as disinfectants and antibiotics are mainly used to treat fish bacterial gill...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/02C12N1/21C12N1/20A61P31/04C12R1/20
Inventor 李宁求林强付小哲刘礼辉郭慧芝吴淑勤
Owner 湖北宜水生物科技有限公司
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