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A live porcine epidemic diarrhea vaccine

A technology for porcine epidemic diarrhea and vaccines, which is applied in the direction of medical preparations with non-active ingredients, digestive system, non-effective ingredients of polymer compounds, etc. It can solve the problems of unsatisfactory clinical application effects and achieve low toxicity and rapid antibody production , good effect of immunogenicity

Active Publication Date: 2015-12-02
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, foreign countries are mainly attenuated vaccines from South Korea, and domestically mainly are porcine transmissible gastroenteritis and epidemic diarrhea inactivated vaccines developed by Harbin Animal Research Institute, but the clinical application effect is not very satisfactory

Method used

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  • A live porcine epidemic diarrhea vaccine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Screening of porcine epidemic diarrhea virus SD10 strain

[0012] 1.1 Isolation and Identification of Porcine Epidemic Diarrhea Virus SD10 Strain During the epidemiological investigation, a strain of porcine epidemic diarrhea virus (named SD10A1 strain) was isolated from the contents of the small intestine of a pig farm with diarrhea in Laixi, Shandong Province. ), porcine epidemic diarrhea virus RT-PCR test was positive, blind transmission to the 8th generation of cytopathy, the 16th generation of regular and stable cytopathy, virus content ≥ 10 5.0 TCID 50 / 0.1ml, can be neutralized by anti-porcine epidemic diarrhea virus-specific serum. When passed to the 121st generation, the passaged virus is safe, pure, and free of bacteria, mold, and mycoplasma for 3-5 day-old healthy piglets that have not fed colostrum and exogenous viruses (classic swine fever, porcine parvovirus, bovine viral diarrhea virus, porcine reproductive and respiratory syndrome, porcine fo...

Embodiment 2

[0038] The safety test of embodiment 2 porcine epidemic diarrhea live vaccine

[0039] 1. Materials and methods

[0040] 1.1 Single dose test

[0041] 1.1.1 Single-dose test on pregnant sows Take 6 pregnant sows that were negative for porcine epidemic diarrhea neutralizing antibodies and antigens 4 to 6 weeks before delivery, and randomly divided them into 2 groups, 3 in each group, the first group The 201001 batches of porcine epidemic diarrhea live vaccines were intramuscularly injected with 1 part per head, and the second group was not injected as the control group, and the sows were observed until the sows gave birth.

[0042] 1.1.2 Single-dose test on piglets Take 12 1-day-old piglets and randomly divide them into 2 groups, 6 pigs in each group. The first group takes 1 batch of 201001 porcine epidemic diarrhea live vaccine orally, and the second group does not Oral as a control group, observed for 14 days.

[0043] 1.2 Single-dose repeated test

[0044]1.2.1 Repeated ...

Embodiment 3

[0053] Efficacy test of embodiment 3 porcine epidemic diarrhea live vaccine

[0054] 1. Active immunization test

[0055] 1.1 Materials and methods Eight sows were randomly divided into 2 groups with 4 pigs in each group. The first group was injected with 201,001 batches of porcine epidemic diarrhea live vaccine in the neck muscle, and the second group was not injected as a control. Group, after the sows gave birth, 14 piglets were taken respectively at the age of 3 days, 7 days, 14 days, and 21 days, and were randomly divided into 2 groups, with 7 pigs in each group. Clinical manifestations of piglets after challenge.

[0056] 1.2 Results The piglets produced by the immunized sows, after the 3-day-old challenge, 7 immunized piglets showed no abnormalities in lactation, spirit, and feces, and were all healthy and alive; after the 7 control piglets were challenged, 7 / 7 showed typical pig epidemics. Sexual diarrhea symptoms, 6 / 7 died. After the 7-day-old challenge, the immune...

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Abstract

The present invention provides a porcine epidemic diarrhea (PED) live vaccine, which contains an antigen and a protection agent, wherein the antigen is a PED virus SD10 strain having the preservation number of CGMCC No.8503. According to the present invention, the used antigen PED virus SD10 strain has characteristics of low toxicity and good immunogenicity, the antibody is rapidly produced after the prepared vaccine is adopted to immunize, the produced antibody has characteristics of high titer, long maintaining time, long storage time and low immune dose, and piglets produced by pregnant sows can achieve good passive immunity through immunization injection before mating, such that the piglets can produce strong immunity and can resist virulent virus attacks so as to improve the piglet survival rate.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, and in particular relates to a porcine epidemic diarrhea live vaccine and application thereof. Background technique [0002] Porcine epidemic diarrhea (Porcineepidemicdiarrhea, PED) is a highly contagious intestinal infectious disease of pigs caused by porcine epidemic diarrhea virus. It is characterized by vomiting, diarrhea and loss of appetite. The disease is susceptible to pigs of different ages Infection, especially in suckling piglets, is the most serious and is a highly contagious infectious disease. The disease first appeared in the United Kingdom in 1971, and occurred successively in my country in the early 1980s. In recent years, the outbreak of porcine epidemic diarrhea in the whole country has caused huge economic losses to the breeding industry. There is currently no effective treatment for this disease infection, mainly based on vaccine immunization prevention. At pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/215A61K47/26A61K47/42A61P31/14A61P1/12
Inventor 陶晓珊刘蕾申洪银邹桂荣邹敏胡潇张维范根成孙健
Owner YEBIO BIOENG OF QINGDAO
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