Method for gradient production of goat anti-Cys-C multiple resistant serum by means of amount of antigens

A technology of antigenicity and cys-c, applied in the direction of serum immunoglobulin, anti-animal/human immunoglobulin, etc., can solve the problems of low yield and titer, unable to meet the use requirements, etc., and achieve high-efficiency results

Inactive Publication Date: 2014-12-17
ZHENJIANG WANSHAN HONGBIAN AGRI PARK
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Problems solved by technology

[0003] Cys-C is an important raw material of biological reagents. The existing production method adopts the tradi

Method used

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Embodiment

[0022] Example: 10 goat rams were used as experimental animals, healthy sheep with a body weight of about 35 kg. Before immunization, all ear tags were put on. Drinking fountains, pasture supply every day, adding concentrate, vinegar grains, bran and vitamins in an appropriate amount.

[0023] Human APOB was inoculated with 0.1ml (combined antigen 4mg) and 1.9ml sterile PBS with complete Freund's (or incomplete) adjuvant 2ml to make immunogen emulsion for later use.

[0024] Goats are immunized with the first injection, front and rear groin, each groin is divided into 2 areas for injection, a total of 12 injection points, subcutaneous injection, and 2 points on both sides of the neck are injected equally.

[0025] The second injection, with an interval of 20 days, was injected in two areas in the front and rear groin, a total of 12 injection points, and two injection points on the left and right of the lymph nodes on both sides of the neck.

[0026] The third injection, with ...

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Abstract

The invention discloses a method for gradient production of goat anti-Cys-C multiple resistant serum by means of amount of antigens. The method comprises the following steps: preparation of an immunogen emulsion: mixing and emulsifying 0.1ml of serum containing 4mg of the Cys-C antigen, 1.9ml of sterile PBS and 2ml of freund adjuvant to obtain an immunogen emulsion; first injection for goal immunity: injecting in two regions of each groin of front and rear groins of a goat, totaling 12 injection points; carrying out subcutaneous injection: performing average injecting at two injection points on two sides of the neck; injecting for the second time every 20 days, injecting for 3-8 doses every 10 days sequentially, and sampling blood from the artery of the immune goat every 4 days; putting the blood in a water tank for 1 hour at 40 DEG C; and after serum is fully separated out, centrifugalizing and precipitating to separate antiserum. The Cys-C antigen is subcutaneously injected to the groins of the goat for booster immunization for many times, so that the generated high-efficient goat anti-Cys-C antibody valence can reach over 1:16.

Description

technical field [0001] The invention relates to a method for producing goat anti-human serum, in particular to a method for producing goat anti-Cys-C multiple antiserum by using antigenic mass gradient. Background technique [0002] Cys-C. is a cysteine ​​protease inhibitor, also known as γ-trace protein and γ-postglobulin, widely present in nucleated cells and body fluids of various tissues, and is a low molecular weight, Basic non-glycosylated protein, with a molecular weight of 13.3KD and composed of 122 amino acid residues, can be produced by all nucleated cells in the body with a constant production rate. [0003] Cys-C is an important raw material of biological reagents. The existing production method adopts the traditional immunization method, and the yield and titer are low, which can no longer meet the growing demand for use. Contents of the invention [0004] Purpose of the invention: In order to overcome the deficiencies of the above-mentioned prior art, the pr...

Claims

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Application Information

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IPC IPC(8): C07K16/18C07K16/06
Inventor 徐志伟刘照亭刘伟忠鲁群
Owner ZHENJIANG WANSHAN HONGBIAN AGRI PARK
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