Method for tissue culture and rapid propagation for rare ellisiophyllum pinnatum (wall.) makino

A kind of tissue culture rapid propagation and rare technology, applied in the field of plant tissue culture, can solve the problems of lack of comprehensive system, no establishment of tissue culture rapid propagation technology system, etc., to achieve the effect of improving seedling efficiency and shortening seedling cycle

Inactive Publication Date: 2014-12-24
ZHENJIANG SUIHAN AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few domestic and foreign reports on the tissue culture rapid propagation of the plant of the genus Poregonium, lack of comprehensive and systematic research, and no establishment of a tissue culture rapid propagation technology system

Method used

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Examples

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Embodiment

[0026] A method for rapid propagation of rare chrysanthemum tissue culture, specifically as follows:

[0027] 1) Take the semi-lignified chrysanthemum branches of the current year as explants, cut the branches into 2‐3cm stems with axillary buds, cut off the leaves, wash off the dust with tap water, soak in detergent solution for 10 minutes, and Rinse under tap water for 1 hour; absorb water with absorbent paper on the ultra-clean workbench, soak in 75% ethanol solution for 30 seconds, then rinse once with sterile water; add 5.6% sodium hypochlorite solution to soak and shake continuously, pour it out after 15 minutes Sodium hypochlorite, then add 0.1% mercury liter to soak for 8-10 minutes, shake it vigorously every 2 minutes, and finally rinse it with sterile water 4-5 times, put it on sterile paper to absorb the water for later use.

[0028] 2) Cut off the sterilized explant wound by 1‐2 mm, insert the end vertically downward into the P1 medium, and transfer it to a new P1 ...

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PUM

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Abstract

The invention discloses a method for tissue culture and rapid propagation for rare ellisiophyllum pinnatum (wall.) makino. The method comprises the steps of (1) selection and disinfection treatment for an explant, (2) sprouting culture, (3) subculture, (4) rooting culture, and (5) domestication, transplantation and the like. According to the method disclosed by the invention, proper culture medium and culture conditions are selected, and a branch is used as the explant for successful rapid propagation. According to the method disclosed by the invention, a technical system for tissue culture and rapid propagation for the plant is established for the first time, and the seedling propagation is not limited by time, seasons and the like, thus greatly shortening the seedling period and improving the seedling efficiency; the seedling period is shortened to four to five months from one year, and the seedling batch number per year is increased to 5 to 10 batches from 2 batches; an effective way is provided for the large-area popularization application.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of a rare chrysanthemum. Background technique [0002] Ellisiophyllum pinnatum (Wall.) Makino is a species of Ellisiophyllum pinnatum (Wall.) Makino, native to North America. The plant likes to be cool and dry, not resistant to heat and humidity, cold, requires sufficient light, and should be fertile and loose soil. It can be sown in autumn or spring. Autumn sowing is carried out in early September, all of which are planted in pots and placed in a low-temperature greenhouse for overwintering for spring and early summer flowering. Spring sowing is carried out in April for viewing flowers in autumn. [0003] Chrysanthemum chrysanthemum is a kind of flower tree with great ornamental value, semi-succulent, with three-pronged branches. Leaves are opposite, obovate-obovate, pinnately parted. Flowers are solitary in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张振超戴忠良潘跃平毛忠良姚悦梅秦文斌潘永飞肖燕王建华马志虎孙春青孙国胜
Owner ZHENJIANG SUIHAN AGRI
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