A method for preparing a purified extract of lonicera japonica thunberg and the composition comprising the same for preventing and treating sepsis and septic shock

A septic shock and extract technology, which is applied in the field of preparing the purified extract of honeysuckle, can solve the problems of unreported or disclosed septicemia therapeutic effect or improvement effect and the like

Inactive Publication Date: 2014-12-24
HUONS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the therapeutic effect or ameliorative effect of the purified flower extract of Lonicera japonica on sepsis is not reported or disclosed in any of the above cited documents, the disclosure of which is incorporated herein by reference.

Method used

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  • A method for preparing a purified extract of lonicera japonica thunberg and the composition comprising the same for preventing and treating sepsis and septic shock
  • A method for preparing a purified extract of lonicera japonica thunberg and the composition comprising the same for preventing and treating sepsis and septic shock
  • A method for preparing a purified extract of lonicera japonica thunberg and the composition comprising the same for preventing and treating sepsis and septic shock

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Embodiment

[0078] The following reference examples, examples and experimental examples are intended to further illustrate the present invention without limiting its scope.

Embodiment 1

[0082] Example 1: Preparation of the Purified Extract of Honeysuckle (HS-23a.23b)

[0083] 1-1. Use 5 times the weight of HP-20 resin (HS-23a)

[0084] The dried flowers of honeysuckle were added to 10 times the volume of distilled water, and extraction was performed at 100° C. for 3 hours by a hot water reflux extraction method. The solution was filtered, the filtrate was concentrated below 60°C with a vacuum evaporator (EYELA N-1000, EYELA Ltd. Japan), and dried with a lyophilizer (FDCF-12012, Operon Co. Korea) to obtain dried crude honeysuckle. Flower Extract. The extract was suspended in 1.5 volumes of water (v / w, based on the weight of the crude extract), and the suspension was fractionated three times with an equal volume of ethyl acetate to remove the ethyl acetate soluble fraction to provide a first purified extract. things. 5 times the weight of water (weight / weight based on the weight of the first purified extract) was added to the extract, and an equal weight of ...

Embodiment 2

[0087] Embodiment 2: the preparation of the purified extract (HS-23c) of honeysuckle

[0088] The dried flowers of honeysuckle were added to 10 times the volume of distilled water, and extraction was performed at 100° C. for 3 hours by a hot water reflux extraction method. The solution was filtered, the filtrate was concentrated below 60°C with a vacuum evaporator (EYELA N-1000, EYELA Ltd. Japan), and dried with a lyophilizer (FDCF-12012, Operon Co. Korea) to obtain dried crude honeysuckle. Flower Extract. The extract was suspended in 1.5 volumes of water (v / w, based on the weight of the crude extract), and the suspension was fractionated three times with an equal volume of ethyl acetate to remove the ethyl acetate soluble fraction to provide a first purified extract. thing. 10 times the weight of water (weight / weight based on the weight of the first purified extract) was added to the extract, and an equal weight of SP-850 resin (Mitsubishi Chemical) was added thereto and st...

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Abstract

The present invention relates to a method for preparing a purified extract of Lonicera Japonica THUNBERG and the composition comprising the same for preventing and treating sepsis and septic shock. The purified extract of purified extract of Lonicera Japonica THUNBERG potent ant-sepsis activity in severe sepsis CLP model test, the effect on MODS, and the inhibitory effect on various pro-inflammatory cytokines such as TNF-alpha, IL-1beta, IFN-gamma, HMGB-1 etc, as well as it showed unexpectedly synergistic effect on the treatment of sepsis and septic shock in case of combining with the commercially available anti-septic agent such as broad-spectrum anti-biotic to the person skilled in the art, therefore, it can be useful in treating and preventing the sepsis and septic shock as a medicament and health functional food.

Description

technical field [0001] The present invention relates to a method for preparing a purified extract of Lonicera Japonica THUNBERG and a composition containing the extract for preventing and treating sepsis and septic shock. Background technique [0002] Severe sepsis is a fatal disease with a high mortality rate resulting from a sepsis condition accompanied by organ failure and hypoperfusion leading to an imbalance between systemic oxygen demand and supply, which rapidly progresses to septic shock and multiple organ dysfunction syndrome (MODS). The typical symptoms of the disease are hyperthermia, hypothermia, positive chronotrophy, increased cardiac output, decreased systemic circulation resistance, respiratory alkalosis, abnormally increased or decreased WBC, etc., which cause Rapid organ dysfunction leading to fatal death. Mortality rates for patients with severe sepsis and septic shock have been reported to be 29% in the United States and 27% in Europe, the leading cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/355A61K36/35A61P29/00
CPCA61K36/355A61K2236/00A23L33/105A61P1/00A61P1/04A61P1/16A61P1/18A61P9/00A61P11/00A61P13/12A61P17/02A61P19/02A61P29/00A61P31/04A61K36/35A61K45/06
Inventor 尹圣泰金桢熏林芳虎金映睦延成钦金炫锈李善美
Owner HUONS
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