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Rapid vegetative propagation technical method for psammosilene tunicoides with axillary buds and immature stems

A technology of tender stems and tender stems, which is applied in the field of asexual rapid propagation of plant traditional Chinese medicine Jintie Suo with axillary buds and tender stems, achieving the effects of less investment, solving the source of seedlings, and reducing pests and diseases

Inactive Publication Date: 2015-01-07
毕节市中药研究所
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Problems solved by technology

At present, there is no report about the rapid propagation of Jin Tiesuo using tender stems with leaves. The present invention has certain innovation and novelty

Method used

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Embodiment 1

[0020] Excavate wild golden iron lock resources with soil, remove all old branches and leaves with a sharp knife at a distance of 1~2cm from the reed head, plant them in a nutrition bag, water them in a timely manner, and place them in a sunny and ventilated place. When new stolons grow, they will be 3~8cm long and When the flower buds do not appear, use a stainless steel blade to cut off the stem section above the first pair of leaves of the newly grown tender stem in the morning on a sunny day, as the source of explant material, gently remove the mud attached to the leaves and stem section with a soft brush, and place Put 3 drops of ordinary detergent and 1 drop of Tween 20 into the clean beaker, cover with gauze and tie it up, rinse it under running water for 2 hours, then put the bottle on the ultra-clean workbench, use 75% Soak in alcohol for 20 seconds, sterilize with 0.1% mercury liter for 6 minutes, soak and wash in sterile water 5 times, 3 minutes each time, blot the r...

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Abstract

The invention discloses a technical method for rapid sterile seedling propagation of psammosilene tunicoides with axillary buds and immature stems. The method comprises the following steps: shearing psammosilene tunicoides with axillary buds and immature stems as an explant, inducing callus by utilizing the explant, differentiating sterile psammosilene tunicoides seedlings by utilizing the callus, performing optimization selection on types, concentration and matching ratios of different plant growth regulators, screening the optimal matching formula of the growth regulator and additives of psammosilene tunicoides in each period according to application of the different natural plant additives and the influence on psammosilene tunicoides induction and a seedling induction effect, thereby constructing a rapid vegetative propagation system of psammosilene tunicoides with the immature stems. According to aseptic seedlings obtained through vegetative propagation, excellent inheritable characters of maternal plants can be maintained to the greatest degree, and aseptic seedlings are provided. By virtue of the technical method, wild psammosilene tunicoides can be prevented from being subjected to extinctive excavation, the propagation coefficient of psammosilene tunicoides seedlings is greatly improved, and thus the method has important significance for protecting the threatened plant, psammosilene tunicoides, and restoring and protecting biological diversity.

Description

technical field [0001] The invention belongs to the technical field of biological vegetative propagation, in particular to the technical method of rapid vegetative propagation of the plant traditional Chinese medicine jintie lock with axillary buds and tender stems. Background technique [0002] Jintiesuo is a plant of the genus Jintiesuo in the caryophyllaceae family, Jintiesuo ( Psammosilene tunicoides W. C. Wu et C. Y. Wu), also known as Dudingzi, Kunming Adenophora, Centipede Seven, Duyeqi, etc., are mainly distributed in Yunnan, Sichuan, Guizhou and parts of Tibet. It is a single-species genus unique to China, and it is an important material for studying the taxonomy and evolution of Caryophyllaceae plants. Folk medicine has a long history. It was first recorded in "Southern Yunnan Materia Medica", and then included in the 1977 edition of "The Pharmacopoeia of the People's Republic of China". It has the functions of dispelling blood stasis, reducing swelling, drai...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈杰张翔宇阮培均李恒谦周茂嫦徐庆祝
Owner 毕节市中药研究所
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