Peony tissue culture and rapid breeding method
A tissue culture and rapid technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow growth of peony, difficult to raise seedlings on a large scale, cumbersome, etc. Low environmental impact, fast effects
Inactive Publication Date: 2015-01-07
LANGXI QINGLIN ECOLOGICAL FEATURED SIGHTSEEING GARDEN
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Problems solved by technology
The growth rate of peonies is slow, the sown seeds will not germinate in the second year, the survival rate is low, and it takes a long period of time. Split-root planting and grafting planting need to be processed one by one, which is cumbersome and affected by seasons and the environment. Very large, it is difficult to grow seedlings on a large scale within a limited time
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Embodiment Construction
[0016] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.
[0017] A rapid breeding method for peony tissue culture, characterized in that: comprising the following steps,
[0018] (1), selection of explants: select the semi-lignified stem section of the current year as the explants;
[0019] (2) Treatment of explants: cut off the explants and the stem tips, keep the leaves with a length of 1-2cm, soak them in saturated detergent for 15-20 minutes, remove the soft hairs on the stems and rinse them with flowing water for 20-20 minutes. 25 minutes, then cut it into 1-2 internode stem segments, soak in 0.1 potassium permanganate aqueous solution for 5 minutes, then rinse with sterile water 4-5 times, and then soak in 0.1% mercury liter aqueous solution Treat for 10 minutes, then rinse with sterile water 5 ti...
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The invention relates to a peony tissue culture rapid breeding method, belonging to the technical field of peony breeding. The peony tissue culture and rapid breeding method is characterized by comprising the following steps: (1) explant selection; (2) explant treatment; (3) culture medium cultivation, namely, culturing 1 / 2MS+6-BA1.1mg / L+NAA0.1mg / L as an induction medium, DCR+6-BA0.7 mg / L+NAA0.1mg / L as a proliferation culture medium and 1.0 mg / L 1 / 2MS+6-IBA0.6mg / L+ABT1 rooting powder as a rooting medium; and (4) seedling exercising and transplanting, namely, exercising seedlings after root bases grow in 90% of cultured tissues in the rooting medium and the length of 60% of roots reaches 0.8-1.2cm, opening a bottle for two-day seedling exercising after seven days, lifting the seedlings and cleaning the culture medium on the roots, soaking the cleaned seedlings in 70% chlorothalonil liquid which is diluted by 1200 times for transplanting, and selecting a transplanting substrate with the ratio of marl soil to yellow soil to calcium magnesium phosphate fertilizer of 2: 2: 0.3. The peony tissue culture rapid breeding method is fast in speed, high in efficiency, low in influence from seasons and environment and convenient for large-scale seedling planting.
Description
technical field [0001] The invention relates to the technical field of peony breeding, and relates to a rapid breeding method of peony tissue culture. Background technique [0002] Peony (Latin scientific name: Paeonia suffruticosa Andr.) is a plant of the family Ranunculaceae and the genus Paeoniae, and is a small perennial deciduous shrub. The flowers are bright in color, fragrant with jade smiles and pearls, romantic and unrestrained, magnificent, known as the "king of flowers". Some rose varieties have very high economic benefits, and belong to famous and precious flower species. In the prior art, the planting methods of peonies generally all adopt split-root planting, sowing planting and grafting planting, etc., sowing planting and split-root planting. The growth rate of peonies is slow, the sown seeds will not germinate in the second year, the survival rate is low, and it takes a long period of time. Split-root planting and grafting planting need to be processed one b...
Claims
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IPC IPC(8): A01H4/00A01G1/00
Inventor 胡庆林
Owner LANGXI QINGLIN ECOLOGICAL FEATURED SIGHTSEEING GARDEN