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Extraction method of shark cartilage for eyedrops

A shark chondroitin and extraction method technology, applied in the field of natural medicinal chemistry, can solve the problems of low product purity, low yield of chondroitin sulfate extraction process, etc.

Active Publication Date: 2015-01-21
JIANGSU QILIKANG SKIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is: the existing chondroitin sulfate extraction process has the disadvantages of low yield and low product purity

Method used

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  • Extraction method of shark cartilage for eyedrops

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for extracting shark chondroitin used in eye drops, comprising the steps of:

[0028] Step 1: Take 10Kg of shark cartilage, crush it into fine powder, add it into 20 Kg of water, stir evenly, and then place it in a distillation kettle for distillation. The temperature of the distillation tower is 120°C, the pressure is 0.3 MPa, and the distillation time is 0.5 hours. , after removing the evaporated steam, let it cool to obtain the extract;

[0029] Step 2: Add dilute sulfuric acid to make the pH of the extract solution 4.0, filter with ultrafiltration membrane, the molecular weight cut off of the ultrafiltration membrane is 200000Da, and then send the ultrafiltration filtrate into the bed filled with alumina particles for adsorption , get permeate;

[0030] Step 3: Use NaOH solution to adjust the pH of the permeate to 10.0, and then send it to the macroporous adsorption resin D101 for adsorption and removal of impurities to obtain the removal of impurities;

...

Embodiment 2

[0034] A method for extracting shark chondroitin used in eye drops, comprising the steps of:

[0035] Step 1: Take 20Kg of shark cartilage, crush it into fine powder, add it into 40 Kg of water, stir evenly, and then place it in a still for distillation. The temperature of the distillation tower is 140°C, the pressure is 0.5 MPa, and the distillation time is 2 hours. , after removing the evaporated steam, let it cool to obtain the extract;

[0036] Step 2: Add dilute sulfuric acid to make the pH of the extract solution 4.0, filter with ultrafiltration membrane, the molecular weight cut off of the ultrafiltration membrane is 200000Da, and then send the ultrafiltration filtrate into the bed filled with alumina particles for adsorption , get permeate;

[0037] Step 3: Use NaOH solution to adjust the pH of the permeate to 10.0, and then send it to the macroporous adsorption resin D101 for adsorption and removal of impurities to obtain the removal of impurities;

[0038] Step 4: ...

Embodiment 3

[0041] A method for extracting shark chondroitin used in eye drops, comprising the steps of:

[0042] Step 1: Take 15Kg of shark cartilage, crush it into fine powder, add it into 30 Kg of water, stir evenly, and then place it in a still for distillation. The temperature of the distillation tower is 130°C, the pressure is 0.4 MPa, and the distillation time is 1 hour. , after removing the evaporated steam, let it cool to obtain the extract;

[0043] Step 2: Add dilute sulfuric acid to make the pH of the extract solution 4.0, filter with ultrafiltration membrane, the molecular weight cut off of the ultrafiltration membrane is 200000Da, and then send the ultrafiltration filtrate into the bed filled with alumina particles for adsorption , get permeate;

[0044] Step 3: Use NaOH solution to adjust the pH of the permeate to 10.0, and then send it to the macroporous adsorption resin D101 for adsorption and removal of impurities to obtain the removal of impurities;

[0045] Step 4: A...

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Abstract

The invention relates to an extraction method of shark cartilage for eyedrops. The extraction method comprises the following steps: taking shark suft bones, grinding into fine powder, adding into water, evenly stirring, placing in a distillation still for distillation, removing evaporative steam, then cooling to obtain extract liquid; adjusting the pH value of the extract liquid to be below 4.0 below, filtering through an ultrafiltration membrane, then conveying the ultrafiltrate into a bed layer filled with alumina grains for adsorption to obtain a permeate solution; regulating the pH of the permeate solution to be higher than10.0, adsorbing by macroporous adsorption resin to remove impurities, thus obtaining a solution with impurities removed; regulating the pH of the solution with impurities removed to 6.0-7.0, performing pressure-reduced concentration, crystallizing, centrifuging and drying to obtain shark cartilage. According to the extraction method, small-molecular impurities in suft bones can be removed in a distillation manner and the fiber structure of the suft bones can be damaged to certain extent, so that the shark cartilage can be separated out as much as possible, and finally high-yield and high-purity shark cartilage can be obtained.

Description

[0001] technical field [0002] The invention relates to a method for extracting shark chondroitin used in eye drops, and belongs to the technical field of natural medicine chemistry. [0003] Background technique [0004] Shark cartilage contains 4 kinds of complex active proteins, chondroitin sulfate and polysaccharides and other active ingredients, which are "anti-tumor angiogenesis growth factors" and "promote human cartilage regeneration". Shark cartilage contains substances that can promote the regeneration of human articular cartilage, such as glucosamine, chondroitin sulfate, collagen and so on. Composed of these substances in a balanced ratio, they can promote the regeneration of human cartilage. The growth and metastasis of tumors depend on the proliferation of new blood vessels to provide nutrients. If the vascular network of tumors is inhibited and destroyed, the growth and development of tumors will be hindered. The "factor" can just prevent the proliferatio...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 吴克
Owner JIANGSU QILIKANG SKIN PHARMA
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