Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of troponin I detection reagent and preparation method thereof

A detection reagent, troponin technology, applied in homogeneous sol particle type cTnI immunoassay reagent and its preparation, troponin I detection reagent and its preparation field, can solve the problems of low initial sensitivity, high specificity, etc. Achieve high analytical sensitivity and high specificity

Inactive Publication Date: 2017-10-03
中国人民解放军第三0五医院
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

cTnI shows lower initial sensitivity and higher specificity than cTnT

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of troponin I detection reagent and preparation method thereof
  • A kind of troponin I detection reagent and preparation method thereof
  • A kind of troponin I detection reagent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] The preparation method of the troponin I detection reagent is as follows:

[0032] 1) Add the coagulant, BSA and preservative to the buffer to prepare reagent one;

[0033] 2) Heat and boil chloroauric acid and trisodium citrate according to the mass ratio of 1:1 to prepare a colloidal gold solution; add cTnI antibody to the colloidal gold solution, and add stabilizers, buffers, and preservatives to prepare reagents Two; Among them, when configuring chloroauric acid and trisodium citrate solution, use a 0.2μm filter membrane to filter;

[0034] 3). The reagent 1 and reagent 2 are used in combination in a volume ratio of 5:1 to obtain a troponin I detection reagent.

Embodiment 1

[0037] Preparation of colloidal gold particles (all the following operations need to be completed in a high-cleanliness and dust-free space)

[0038] 1) Preparations: All the glass containers used are washed with detergent and rinsed with running water, and then soaked with siliconizing agent overnight. All the inner surfaces of the goldware are siliconized, and then rinsed with distilled water for use;

[0039] 2) Prepare 1% (w / v) chloroauric acid and 1% (w / v) sodium citrate solution and filter with 0.2μm filter membrane;

[0040] 3) Put a magnetic stir bar in a clean 1000ml round bottom flask and add 1000ml ultrapure water;

[0041] 4) Add a certain volume concentration of 1% (w / v) chloroauric acid, stir at a high speed around 600 rpm, and heat until the solution boils, then quickly add a certain volume concentration of 1% sodium citrate solution to the flask and keep heating Stir vigorously for 10 minutes, the color of the solution first turns black, then gradually turns purple red...

Embodiment 2

[0045] Preparation of reagent one

[0046] The formula of reagent one is as follows: Tris-Hcl buffer, 0.9% NaCl (w / v), 2% BSA (w / v), 0.1% NaN3 (w / v), 0.05% Tween20 (w / v), according to The concentration of Tris quality control buffer is adjusted according to the amount of HCl used to adjust the pH of the buffer.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a troponin I detection reagent and a preparation method thereof. The troponin I detection reagent is prepared from a first reagent and a second reagent in the volume ratio of 5: 1, wherein the first reagent contains 20-50mmol / L of buffer solution, 2%w / v of coagulant, 2%w / v of BSA and 0.1%-0.5%w / v of preservative; the second reagent contains 0.1%-0.5%w / v of colloid gold particles marked with anti-human cTnI antibodies, 2%-5%w / v of stabilizer, 20-50mmol / L of buffer solution and 0.1%-0.5%w / v of preservative; the diameter of the colloid gold particles marked with the anti-human cTnI antibodies ranges from 60nm to 90nm. The troponin I detection reagent has the advantages of being good in operability, high in analytical sensitivity, high in linearity, and quick, simple and convenient.

Description

【Technical Field】 [0001] The invention relates to a troponin I detection reagent and a preparation method thereof, in particular to a homogeneous sol particle type cTnI immunoassay reagent for detecting cTnI in serum or plasma and a preparation method thereof, and belongs to in vitro diagnostics in medical devices field. 【Background technique】 [0002] Troponin I has a molecular weight of 23,000 and is an actin inhibitory subunit. It has three subtypes: fast skeletal muscle subtype, slow skeletal muscle subtype and cardiac muscle subtype. The three TnI subtypes are derived from three different genes. Myocardial subtype (cTnI) has about 40% of the release form of cTnI after AMI (free form or combined with other troponins, oxidized form or reduced form) compared with two skeletal muscle subtypes. Not completely clear. [0003] Diagnosis of myocardial injury Among many clinical biochemical indicators for the diagnosis of acute myocardial infarction (AMI), CK-MB (serum myocardial en...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6887
Inventor 王会中
Owner 中国人民解放军第三0五医院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com