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Viral immunotherapy drug compound and purpose thereof

A technology of immunotherapy drugs and antiviral drugs, applied in the field of biomedicine, can solve the problems of ineffective improvement of primary immune response, GM-CSF cannot provide adjuvant activity, etc., to prevent re-infection, easy to promote, and enhance immune response Effect

Inactive Publication Date: 2015-02-11
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hasan et al found that GM-CSF cannot provide significant adjuvant activity by intramuscularly injecting GM-CSF immediately before injecting recombinant hepatitis B vaccine to normal people, that is, it cannot effectively improve the primary immune response[3]

Method used

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  • Viral immunotherapy drug compound and purpose thereof
  • Viral immunotherapy drug compound and purpose thereof
  • Viral immunotherapy drug compound and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Unless otherwise specified, the experimental data in the examples of the present invention are the average values ​​obtained from each mouse in each group. Example 1: The immunization strategy of GM-CSF combined with recombinant hepatitis B vaccine enhances the immune response of C57BL / 6 to recombinant hepatitis B subunit vaccine

[0063] Materials and Instruments:

[0064] Experimental materials: Genetic engineering (CHO) hepatitis B vaccine [Recombinant Hepatitis B Vaccine (CHO)], 10ug / 1.0mL, recombinant human granulocyte macrophage colony-stimulating factor for injection [Recombinant Human Granulocyte / Macrophage Clolony-Stimulating Factor for Injection], 300ug / support, CHO-expressed genetically engineered hepatitis B vaccine stock solution (HBsAg stock solution) were provided by North China Pharmaceutical Group Jintan Biotechnology Co., Ltd.

[0065] Main kits and instruments: RPMI1640 culture medium (WISENT company); fetal bovine serum (Tianjin Haoyang Biological P...

Embodiment 2

[0131] Example 2: The immunization strategy of GM-CSF combined with recombinant hepatitis B vaccine breaks the immune tolerance of HBsAg transgenic mice and induces anti-HBsAg humoral immune response in transgenic mice

[0132] Materials and Instruments:

[0133] HBsAg transgenic mice (C57BL / 6J-Tg(AlblHBV)44Bri / Jf4J) were purchased from Shanghai Public Health Clinical Center affiliated to Fudan University. "Hepatitis B surface antigen enzyme-linked immunoassay diagnostic kit" and hepatitis B surface antigen standard were purchased from Beijing Jinhao Pharmaceutical Co., Ltd. Other experimental materials, main reagents and instruments are the same as in Example 1.

[0134] experiment method:

[0135] Animal grouping and immunization methods:

[0136] 35 HBsAg transgenic mice (C57BL / 6J-Tg(AlblHBV)44Bri / Jf4J) (initial serum HBsAg concentration is 5000-10000pg / ml), were randomly divided into 5 groups, 7 mice in each group, and the experimental grouping was performed according ...

Embodiment 3

[0157] Example 3: GM-CSF Combined with Recombinant Hepatitis B Vaccine Immune Strategy Breaks Immune Tolerance of HBsAg Transgenic Mice Induces Anti-HBsAg Cellular Immune Response in Transgenic Mice and Eliminates HBsAg in Liver

[0158] Materials and Instruments:

[0159] HBsAg transgenic mice (C57BL / 6J-Tg(AlblHBV)44Bri / Jf4J) were purchased from Shanghai Public Health Clinical Center affiliated to Fudan University. The primary antibody and secondary antibody of hepatitis B surface S antigen immunohistochemistry were purchased from Shanghai Changdao Biotechnology Co., Ltd. Other experimental materials, main reagents and instruments are the same as in Example 1.

[0160] Animal grouping and immunization methods:

[0161] Thirty-five HBsAg transgenic mice (C57BL / 6J-Tg(AlblHBV)44Bri / Jf4J) (initial serum HBsAg concentration was 5000-10000pg / ml) were randomly divided into 5 groups with 5 mice in each group. The experimental groups are shown in the table below. Each group of vacc...

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Abstract

The present invention involves a new viral immunotherapy drug complex, and in particular, a viral immunotherapy drug complex for persistent hepatitis B infection. The present drug consists of antiviral drugs, immunoregulating drugs and a recombinant hepatitis B vaccine, for use in treating hepatitis B and in particular chronic hepatitis B infections. Antiviral drugs of the described drug complex are selected among α-IFN and nucleosides; the immunoregulating drugs are selected from among GM-CSF and similar.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a novel drug compound for virus immunotherapy. In particular, it relates to a drug compound for virus immunotherapy for persistent hepatitis B virus infection. Background technique [0002] The prior art discloses that hepatitis B is an infectious disease caused by hepatitis B virus (HBV), transmitted through blood and body fluids, and mainly based on liver damage. It is a serious public health problem, and the disease is harmful to human health. The threat is great. Studies have shown that after infection with hepatitis B, some patients will develop into a state of chronic persistent infection, which may evolve into liver cirrhosis or primary hepatocellular carcinoma. my country is a high prevalence area of ​​hepatitis B virus infection. About 350,000 people die of hepatitis B-related diseases (such as liver cirrhosis and liver cancer) every year. Among them, the infection rate of the ...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K39/29A61K39/39A61P31/20A61P1/16
CPCA61K2039/55522A61K2039/545A61K45/00C12N2730/10134A61K39/39A61K39/292A61K38/193A61K38/21A61K31/513A61K31/522A61K31/675A61K31/7072A61K45/06A61K39/12A61K2300/00A61K47/60A61K38/212A61P1/16A61P31/20A61P37/04A61P43/00C12N7/00
Inventor 王宾王宪政张继明
Owner FUDAN UNIV
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