Method for measuring chitosan oligosaccharide content in specific polymerization degree range
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A specific degree of polymerization, chitosan oligosaccharide technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficulties in practical application of the method, difficulty in obtaining standard products, etc., and achieve simple operation, few analysis steps, and operability strong effect
Inactive Publication Date: 2015-02-11
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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However, due to the lack of a single degree of polymerization chitosan standard for calibration, especially for chitosan monomers with a polymerization degree greater than 6 and N-acetylated chitosan monomers with a specific sequence arrangement, it is still difficult to obtain standards , which brings difficulties to the practical application of the method
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Embodiment 1
[0023] Determination of the content of oligosaccharides with a degree of polymerization of 2-10 in the chitosan oligosaccharide sample:
[0024] Take by weighing 4 mg of dextran standard substances with molecular weights of 180, 1000, 2500, 5000, 7100, and 12000 respectively, and dissolve them in 1 ml of acetic acid-sodium acetate buffer solution (the concentration of acetic acid in the buffer solution is 0.2mol / L, and the concentration of sodium acetate is 0.1mol / L), adopt high performance gel exclusion chromatography to measure respectively the chromatographic retention time of each dextran standard substance, and the chromatographic condition is:
[0029] The chromatographic retention times of dextran standard products with corresponding molecular weights of 180, 1000, 2500, 5000, 7100 a...
Embodiment 2
[0033] Determination of the content of oligosaccharides with a degree of polymerization of 3-8 in chitosan oligosaccharide samples
[0034] Take by weighing 4 mg of dextran standard substances with molecular weights of 180, 1000, 2500, 5000, 7100, and 12000 respectively and dissolve them in 1 ml of acetic acid-sodium acetate buffer solution (the concentration of acetic acid in the buffer solution is 0.2 mol / L, and the concentration of sodium acetate is 0.1 mol / L). In L), adopt high performance gel exclusion chromatography to measure respectively each dextran standard substance chromatographic retention time, chromatographic condition is:
[0039]The chromatographic retention times of dextran standard products with corresponding molecular weights of 180, 1000, 2500, 5000, 7100 and 12000 obtai...
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Abstract
The invention belongs to an ocean chemical engineering technology, and particularly relates to a method for measuring the chitosan oligosaccharide content in a specific polymerization degree range. According to the method, a dextran standard product is subjected to chromatographic analysis, a standard curve is drawn, and a linear regression equation is obtained; chitosan oligosaccharide molecular weights M1 and M2 corresponding to two end values n1 and n2 of the specific polymerization degree range to be measured are substituted into the linear regression equation, and the chitosan oligosaccharide chromatographic retention time t1 and t2 of the two end values of the specific polymerization degree range to be measured; a chitosan oligosaccharide solution to be measured is analyzed by measuring the chromatographic analysis condition of the standard sample, the chromatographic peak total area A corresponding to the sample to be measured and the chromatographic area A<t1-t2> in the chitosan oligosaccharide chromatographic retention time t1 to t2 of the two end values of the specific polymerization degree range to be measured are obtained through a chromatogram of the sample to be measured, and the chitosan oligosaccharide content of the specific polymerization degree range n1 to n2 is obtained according to a chromatographic area formula. The method has the advantages that the chitosan oligosaccharide content of the specific polymerization degree is indirectly measured by using the molecular weight as a calibration measure through gel exclusion chromatography, the analysis steps are few, and the operation is simple and convenient.
Description
technical field [0001] The invention belongs to marine chemical engineering technology, in particular to a method for measuring the content of chitosan oligosaccharide in a specific polymerization degree range. Background technique [0002] Oligochitosan, also known as oligoglucosamine and oligoglucosamine, is a linear oligosaccharide formed by connecting D-glucosamine and N-acetyl-D-glucosamine through β-1,4 glycosidic bonds. Oligochitosan is an important marine biological resource, which has been found to have a variety of physiological activities, such as anti-tumor, anti-bacterial, anti-inflammatory, anti-oxidation, regulating blood sugar and blood lipids, enhancing immunity, activating intestinal flora, etc. In May 2014, the National Health Commission has approved chitosan oligosaccharide as a new food raw material, which is of great significance to the development of chitosan oligosaccharide industry. At present, chitosan oligosaccharides are mainly obtained by acid h...
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