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Dye sets for surface enhanced resonant raman spectroscopy

A technology of Raman spectroscopy and dyes, applied in the field of dye groups, can solve problems such as difficult identification and analysis

Inactive Publication Date: 2015-02-11
RENISHAW DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, many potent SERRS dyes share significant chemical similarities, resulting in many dyes having the same features in their SERRS spectra, making them more difficult to resolve in mixtures

Method used

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  • Dye sets for surface enhanced resonant raman spectroscopy
  • Dye sets for surface enhanced resonant raman spectroscopy
  • Dye sets for surface enhanced resonant raman spectroscopy

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Embodiment Construction

[0062] figure 1 A method for selecting a panel of dyes that can be used in conjunction with multichannel diagnostic applications is shown. The method includes five steps.

[0063] The first step involves preselecting N dyes in a dye pool, where the dyes are evaluated according to the following criteria:

[0064] a) The dye signal should be stable and unaffected by the presence of other dyes.

[0065] b) The dye signal should show a linear response to concentration, obeying the Beer-Lambert law.

[0066] c) Spectral distribution diagram of dye SERRS. The profile must be unique and ideally the dye should show a strong SERRS signal with low fluorescence background. Optimum dyes exhibit at least one distinguishing spectral characteristic.

[0067] d) Dye chemical stability.

[0068] e) Dye kinetic range. The SERRS profile of the dye should be detectable across a wide range of concentrations.

[0069] f) Dye chemical affinity. For example, the dye should provide high chemi...

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Abstract

This invention concerns a kit for use in a multiplex assay, the kit comprising a dye set consisting essentially of a plurality of dyes and an association of each dye to a reference concentration. The dye set has been selected such that, using surface enhanced resonant Raman spectroscopy, each dye of the set is identifiable at better than 90% sensitivity and 90% specificity in the presence of any other dye of the set throughout a range of concentrations of each of the two dyes from 0.6 to 1.5 of the respective dye's reference concentration. In one embodiment, thedye set consistsof of the following dyes: JOE, Rhodamine Green, ATTO520, BODIPY FL, BODIPY TMR-X,FAM, HEX, Cy3, Cy3.5, TAMRA and TYE563. The invention also concerns a multiplex assay comprising the set of dyes, apparatus for carrying out the multiplex assayand a method of selecting the set of dyes.

Description

technical field [0001] The present invention relates to a set of dyes for use in a multiplex assay and a method for selecting said set of dyes, wherein said dyes are identified using surface enhanced resonant Raman spectroscopy (SERRS). The invention also relates to a multi-channel analysis and a device for carrying out said multi-channel analysis. Background technique [0002] Multiplexing in a laboratory setting is defined as the simultaneous detection of multiple analytes in a single interrogation. In general, the advantage is that many more data points are generated and a higher degree of information can be gathered. This can result in a substantial reduction in time that can be achieved by searching for multiple targets in a single test, rather than performing multiple serial analyses. High-throughput multi-channel analysis means easy and convenient large-scale testing. In addition, the physical volume of samples required for full panel testing is significantly reduc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658G01N2201/1293C12Q1/6809C12Q1/6851G01N21/25
Inventor 阿拉斯泰尔·里基茨卡伦·菲切特朱莉·格林格雷姆·麦克内伊布赖恩·史密斯托马斯·瑟斯顿伊恩·贝尔安德鲁·伍尔夫雷伊
Owner RENISHAW DIAGNOSTICS
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