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Extraction and purification method of purple potato anthocyanin

A purification method, purple potato technology, applied in the direction of organic chemistry, can solve the problems of low purity and inability to meet the wide application of anthocyanins, and achieve the effect of high product purity

Active Publication Date: 2015-02-18
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, when the above extraction process is applied to the extraction of anthocyanins from purple potatoes, the purity is still low, which cannot meet the wide application of anthocyanins in the fields of health food and medicine.

Method used

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  • Extraction and purification method of purple potato anthocyanin

Examples

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Comparison scheme
Effect test

Embodiment 1

[0039] A method for extracting and purifying purple potato anthocyanins, comprising the following steps:

[0040] (1) 500g of fresh and non-rotten purple potatoes are washed with pure water, and cut into pieces to obtain processed purple potatoes;

[0041] After the processed purple potato is mixed with 1000g mass concentration of 1.5% citric acid aqueous solution, it is beaten through beating and filtered through a 80-mesh sieve, and the filter residue is mixed with 1000g mass concentration of 1.5% citric acid aqueous solution. Sieve filtration, merge the filtrate of secondary filtration;

[0042] Centrifuge the filtrate obtained above at 5000r / m for 5 minutes, take the supernatant, wash the precipitate twice, combine the supernatant and the washing liquid to obtain the potato anthocyanin extract, and dry the precipitate at 80°C to produce get potato starch;

[0043] The above washing steps are as follows: add 200 ml of citric acid aqueous solution with a mass concentration...

Embodiment 2

[0051] A method for extracting and purifying purple potato anthocyanins, comprising the following steps:

[0052] (1) 500g of fresh and non-rotten purple potatoes are washed with pure water, and cut into pieces to obtain processed purple potatoes;

[0053] After the processed purple potato is mixed with 1000g mass concentration of 1% citric acid aqueous solution, it is beaten through beating and filtered through a 80-mesh sieve, and the filter residue is mixed with 1000g mass concentration of 1% citric acid aqueous solution, beaten, and filtered through an 80-mesh sieve. Sieve filtration, merge the filtrate of secondary filtration;

[0054] Centrifuge the filtrate obtained above at 5000r / m for 5 minutes, take the supernatant, wash the precipitate once, combine the supernatant and washing liquid to obtain the potato anthocyanin extract, and dry the precipitate at 80°C to produce get potato starch;

[0055] The above washing steps are as follows: add 200 ml of citric acid aque...

Embodiment 3

[0063] A method for extracting and purifying purple potato anthocyanins, comprising the following steps:

[0064] (1) 500g of fresh and non-rotten purple potatoes are washed with pure water, and cut into pieces to obtain processed purple potatoes;

[0065] After the processed purple potato is mixed with 1000g mass concentration of 2% citric acid aqueous solution, it is beaten through beating and filtered through a 80-mesh sieve, and the filter residue is mixed with 1000g mass concentration of 2% citric acid aqueous solution, beaten, and filtered through 80 mesh Sieve filtration, merge the filtrate of secondary filtration;

[0066] The filtrate obtained above was centrifuged at 5000r / m for 5 minutes, the supernatant was taken, and the precipitate was washed 3 times. get potato starch;

[0067] The above washing steps are as follows: add 200 ml of citric acid aqueous solution with a mass concentration of 2% to the precipitate, mix well, and centrifuge at 5000 r / m for 5 minutes...

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Abstract

The invention relates to an extraction and purification method of purple potato anthocyanin. The method comprises the following steps: mixing treated purple potatoes with citric acid aqueous solution, pulping, filtering, centrifuging a filtrate, precipitating supernatant liquid taken as the purple potato anthocyanin, washing and drying to obtain potato starch; performing absorbing on a potato extract with macroporous resin and desorbing with ethanol solution to obtain a potato anthocyanin rough extract; performing absorbing on the potato anthocyanin rough extract with cation exchange resin, removing micro-molecular impurities which are not positively charged under the acidic conditions, performing absorbing with anion exchange resin again, removing impurities which are not negatively charged under the alkaline conditions, desorbing, decompressing and concentrating, freezing and drying to obtain the purple potato anthocyanin. The extraction and purification method has the benefits that both the potato anthocyanin and the purple potato anthocyanin are simultaneously obtained, the purity of the obtained potato anthocyanin is high, the added value of the purple potatoes is improved, and the comprehensive utilization of the purple potatoes is realized.

Description

technical field [0001] The invention relates to a method for extracting and purifying purple potato anthocyanins, and belongs to the technical field of deep processing of agricultural by-products. Background technique [0002] Purple potato is a new potato variety newly introduced in my country in recent years. It has the function of both medicine and food because it is rich in potato anthocyanins. As a natural food coloring, it also has various pharmacological effects such as strong anti-oxidation, anti-mutation, prevention of cardiovascular and cerebrovascular diseases, and liver protection. The value of development and utilization of purple potato anthocyanins has gradually been valued. , cosmetics and pharmaceutical industries have broad application prospects. [0003] Chinese patent document CN102020868A (application number 201010179142.0) discloses a technology for extracting natural pigments, and specifically relates to a method for extracting anthocyanin pigments fr...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 刘可春韩利文袁延强彭维兵何秋霞楚杰陈锡强
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI