Method for extracting collagen by using ecdysis of giant salamander

A collagen and giant salamander technology, applied in the biological field, can solve problems such as resource waste and skin waste, and achieve significant progress

Inactive Publication Date: 2015-02-18
四川龙王洞生态农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for extracting collagen by using the molted skin of giant salamanders in view of the problem that the molted skin of the giant salamander is wasted in vain, resulting in a serious waste of resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Pretreatment

[0035] The molting of giant salamander is used as raw material, which is recorded as raw material molting. The raw material molting is soaked and pretreated with a dilute hydrochloric acid solution with a mass percentage of 2.0%, and then the soaked molting is washed with purified water 8 times the weight of the raw material molting for 5.5 hours, and then the washed molting is minced to obtain a minced molting . After mixing the minced molting skin with water at a weight ratio of 1:1, heat treatment is carried out at a heating temperature of 98-100°C and a heating time of 30 minutes to obtain a sterilized and clean molting skin.

[0036] (2) Rough extraction

[0037] Add sterilizing and cleaning molting to water, and then add compound enzyme with 0.6% mass of sterilizing and cleaning molting to obtain the first solution, adjust the pH value of the first solution to 6.6, and perform enzymatic hydrolysis at 40°C for 4 hours, then raise the temperatur...

Embodiment 2

[0046] (1) Pretreatment

[0047] The molting of giant salamander is used as raw material, which is recorded as raw material molting. The raw material molting is soaked and pretreated with 5.0% dilute hydrochloric acid solution by mass percentage, and then the soaked molting is washed with purified water 10 times the weight of the raw material molting for 8 hours, and then the washed molting is minced to obtain minced molting . After mixing the crushed molted skin with water at a weight ratio of 1:0.9, heat treatment is carried out at a heating temperature of 98-100°C and a heating time of 40 minutes to obtain sterilized and clean molted skin.

[0048](2) Rough extraction

[0049] Add sterilizing and cleaning molting to water, and then add 0.8% compound enzyme of sterilizing and cleaning molting to obtain the first solution, adjust the pH value of the first solution to 6.9, and perform enzymatic hydrolysis at 45°C for 3 hours, then raise the temperature to 118°C Inactivate t...

Embodiment 3

[0058] (1) Pretreatment

[0059] The molting of giant salamander is used as raw material, which is recorded as raw material molting. The raw material molting is soaked and pretreated with a dilute hydrochloric acid solution with a mass percentage of 4.0%, and then the soaked molted skin is washed with purified water 8 times the weight of the raw material molted skin for 7 hours, and then the washed molted skin is minced to obtain a minced molted skin . After mixing the minced molting skin and water at a weight ratio of 1:1, heat treatment is carried out at a heating temperature of 98-100°C and a heating time of 40 minutes to obtain a sterilized and clean molting skin.

[0060] (2) Rough extraction

[0061] Add sterilizing and cleaning molting to water, and then add compound enzyme with 0.8% mass of sterilizing and cleaning molting to obtain the first solution, adjust the pH value of the first solution to 6.8, and perform enzymatic hydrolysis at 45°C for 2 hours, then raise t...

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PUM

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Abstract

The invention discloses a method for extracting collagen by using ecdysis of giant salamander. The method aims at solving the problem that the ecdysis of the giant salamander is wasted so that the resource is seriously wasted in the parturition process of the giant salamander. The method comprises the following steps: pre-treating, coarsely extracting, deodorizing, extracting and purifying collagen. The collagen is finally obtained by treating, performing enzymolysis, filtering, concentrating and spray-drying the ecdysis of the giant salamander in artificial breeding, and the molecular weight is less than 3000 dalton. The ecdysis of the giant salamander is effectively utilized, and the collagen is successfully prepared. The method has a wide market application prospect and good social and economic value.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for extracting collagen by molt of giant salamander. Background technique [0002] Giant salamander, commonly known as giant salamander, is an endangered animal in the world and a second-class protected animal in the country. It has become a unique species in my country and is only distributed in a few areas of our country. Giant salamander muscle protein contains 17 kinds of amino acids, 8 of which are essential amino acids for human body. The EAAI (Essential Amino Acid Index) in its muscle is greater than that of beef and venison. At present, artificial breeding of giant salamanders has begun in my country. During the growth process of giant salamanders, giant salamanders will molt many times. Currently, the skins are disposed of as waste and are not fully utilized. [0003] At present, there are many methods for extracting collagen from fish skin in China, but no method has b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/14
Inventor 邱平阳爱生田宇
Owner 四川龙王洞生态农业开发有限公司
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