Giant salamander active peptide and hyaluronic acid composition for effectively promoting fibroblast cell proliferation

A technology of fibroblasts and active peptides is applied in the field of skin care products to achieve the effects of improving the proliferation of skin fibroblasts, promoting the proliferation of skin fibroblasts, and having broad application prospects.

Active Publication Date: 2019-12-31
深圳润朗医药科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0012] However, so far, there is no report on the combination of giant salamander active peptide solution and hyaluronic acid to improve the effect of promoting fibroblast proliferation

Method used

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  • Giant salamander active peptide and hyaluronic acid composition for effectively promoting fibroblast cell proliferation
  • Giant salamander active peptide and hyaluronic acid composition for effectively promoting fibroblast cell proliferation
  • Giant salamander active peptide and hyaluronic acid composition for effectively promoting fibroblast cell proliferation

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Embodiment Construction

[0025] The Chinese giant salamander active peptide and hyaluronic acid composition that can effectively promote the proliferation of fibroblasts of the present invention is prepared by mixing a giant salamander active peptide solution with a mass volume concentration of 8% and a pH of 6 with hyaluronic acid at a mass ratio of 5:1. The molecular weight of the giant salamander active peptide is Less than 2000Da, the molar percentages of lysine and arginine are 7.2% and 9.7% respectively, and the molecular weight of hyaluronic acid is 80kDa. The giant salamander active peptide solution with a mass volume concentration of 8% and a pH of 6 is prepared by adding water to the giant salamander active peptide solution to form a mass volume 8% (w / v) giant salamander active peptide solution, and the pH is adjusted to 6 with dilute hydrochloric acid.

[0026] Giant salamander active peptide is prepared according to the following steps:

[0027] a. Homogenize giant salamander meat, add 2 t...

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Abstract

The present invention discloses a giant salamander active peptide and hyaluronic acid composition for effectively promoting fibroblast cell proliferation. A mass ratio of a giant salamander active peptide solution at a mass volume concentration of 1-10% and pH of 5-7 to hyaluronic acid is (1-8):1, molecular weight of the giant salamander active peptide is less than 2,000 Da, mole percentage of lysine and arginine are 7.2% and 9.7%, respectively, and molecular weight of the hyaluronic acid is 42.5-145 kDa. The giant salamander active peptide can be naturally cross-linked with the hyaluronic acid rich in multiple hydroxyl groups through hydrogen bonding, natural affinity of the hyaluronic acid and human skin cells is used, and the giant salamander active peptide and hyaluronic acid composition can effectively promote promotion of skin fibroblast proliferation and has broad application prospects in the fields of medicines and cosmetics,.

Description

technical field [0001] The invention belongs to the technical field of skin care products, in particular to a giant salamander active peptide and hyaluronic acid composition that can effectively promote the proliferation of fibroblasts. Background technique [0002] Bioactive peptides are mainly the products of enzymatic hydrolysis of proteins by enzymes. Different enzymes and enzymatic hydrolysis methods are used to enzymatically hydrolyze proteins from the same source, and the hydrolyzed products will also have different biological activities due to different structures. Fu Jing et al. studied the peptide yield of giant salamander meat enzymatically hydrolyzed by five kinds of enzymes including alkaline protease, neutral protease, Flavourzyme, Protamex and Alcalase, and found that the peptide yield obtained by enzymatically hydrolyzing giant salamander meat with Protamex was the highest, which was 13.75%. The optimum conditions for enzymatic hydrolysis of giant salamander ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/73A61K8/64A61Q19/00A61K38/01A61K31/728A61P17/00C12P21/06C07K1/34C07K1/16C07K1/14
CPCA61K8/735A61K8/64A61Q19/00A61K38/012A61K31/728A61P17/00C12P21/06C07K1/34C07K1/16C07K1/14A61K2300/00
Inventor 李伟佟长青
Owner 深圳润朗医药科技有限公司
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