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Injection type tissue engineering bone renovation material and construct method thereof

A tissue-engineered bone and repair material technology, used in tissue culture, biochemical equipment and methods, tissue cell/virus culture devices, etc. Easy to prepare, inexpensive effect

Inactive Publication Date: 2007-03-07
NAN FANG HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, a single growth factor was often used for research, but a single growth factor cannot exert the synergistic effect of multiple factors, and its effect is relatively limited
The osteogenic effect of multi-factor combined application is better than that of a single growth factor, but how to match different growth factors and the optimal ratio between different growth factors need to be studied, and purified growth factors exist in different degrees Complicated preparation, high cost, certain immunogenicity and other disadvantages
Therefore, although growth factors have achieved a lot of research results in the field of orthopedic basic research, they still cannot be widely used clinically.

Method used

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  • Injection type tissue engineering bone renovation material and construct method thereof
  • Injection type tissue engineering bone renovation material and construct method thereof
  • Injection type tissue engineering bone renovation material and construct method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment one: the cultivation of seed cell

[0031] Take 5-month-old healthy Chinese green goats (weight 35.0-40kg, male or female, provided by the Animal Experiment Center of Southern Medical University), perform iliac crest puncture with a 16-gauge bone puncture needle under aseptic operation after anesthesia, and extract bone marrow by multi-point puncture 10mL, centrifuged and filtered with 100-mesh stencil, inoculated into a culture bottle, complete medium (DMEM+10% fetal bovine serum), placed in CO 2 Cultivate in an incubator (Harris Company, USA), and change the medium every 3 days. After the primary cells cover the bottom of the bottle, digest and separate the adherent cells with 0.25% trypsin-0.02% DMEM solution, add complete medium, and place in CO 2 The incubator continued to cultivate, and the medium was changed every 2-3 days.

Embodiment 2

[0032] Embodiment 2: Preparation of platelet-rich plasma (PRP)

[0033] Take 10ml of sheep venous blood with a syringe containing 1ml 10% sodium citrate anticoagulant, shake it up, put it in a centrifuge tube, and prepare PRP by secondary centrifugation (Huang Aiwen, Jin Dan, Pei Guoxian, Chen Shujun, Hu Jijie, Lin Haining, Zeng Xianli, Construction of injectable tissue-engineered bone compounded with platelet-rich plasma (PRP) and its radiological evaluation for repairing radial defect in rabbits, Chinese Journal of Traumatic Orthopedics, 2005, 7(4): 363-367.). Make about 1ml of PRP per 10ml of whole blood and store in a -70°C refrigerator for later use.

Embodiment 3

[0034] Example 3: Preparation of Injectable Tissue Engineering Bone Repair Material

[0035] The third generation BMSCs (planting density 2×104 / well) were inoculated in 24-well culture plates, and 0.6ml of culture medium was added to each well.

[0036] Injectable chitosan and β-tricalcium phosphate (β-TCP) materials were provided by the Department of Materials Science and Engineering, Jinan University. After 3ml chitosan and 1.8gβ-TCP were compounded in vitro for 8min, 0.3mlPRP and 0.1ml thrombin (10% calcium chloride contained 100U thrombin) were added into the liquid chitosan-β-TCP successively. Mix well to prepare chitosan-β-TCP / PRP complex.

[0037]The preparation experiment of injection-type tissue engineering bone repair materials was divided into three groups, namely chitosan-β-TCP / PRP group, simple chitosan-β-TCP group and blank control group. Among them, chitosan-β-TCP / PRP group and simple chitosan-β-TCP group added 5mm×5mm×3mm solidified chitosan-β-TCP / PRP or simp...

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Abstract

The invention relates to an injection organism repair material and relative production. Wherein, it comprises carrier support and seed cell adhered on the carrier support to form skeleton three-dimension structure and biological active composite; and it uses the injection chitose-beta-tricalcium phosphate composite as carrier support to couple seed cell; the seed cell is bone marrow substrate stem cell. The test has proved that the chitose-beta-tricalcium phosphate composite has better compatibility with BMSCs.

Description

technical field [0001] The invention belongs to the technical field of constructing artificial organs by tissue engineering method in biomedical engineering, and in particular relates to an injection-type tissue engineering bone and a construction method thereof. Background technique [0002] At present, tissue engineered bone is mostly constructed as a model in which the scaffold material is loaded with cell growth factors and then compounded with seed cells. The selection of scaffold materials and growth factors is the focus of tissue engineering research. Scaffold materials, as seed cell carriers, have the functions of loading cells and slowing the release of growth factors. At present, most commonly used scaffold materials are solid materials, which have achieved remarkable results in the construction of tissue engineered bone. However, there are also defects such as difficulty in shaping, complex operation of loading growth factors and seed cells, low loading rate, and...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/12A61L27/38A61L31/00C12M3/00C12N5/00
Inventor 裴国献金丹程文俊
Owner NAN FANG HOSPITAL
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