Method for promoting growth of probiotics by adding rutin in culture medium

A technology of probiotics and culture medium, applied in the direction of microorganism-based methods, adding compounds to stimulate growth, biochemical equipment and methods, etc., to achieve obvious proliferation effects, increase growth and viable bacteria count, and promote growth effects

Active Publication Date: 2015-11-04
吉林省浦生泰生物技术有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no literature report on the proliferative effect of rutin on probiotics, and there is no report on the probiotics added with rutin

Method used

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  • Method for promoting growth of probiotics by adding rutin in culture medium
  • Method for promoting growth of probiotics by adding rutin in culture medium
  • Method for promoting growth of probiotics by adding rutin in culture medium

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Embodiment Construction

[0022] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0023] (1) Preparation of rutin solution: add rutin into DMSO, stir until dissolved, and sterilize at 115 °C for 30 min. The rutin monomer (purity 99%) was configured as a DMSO concentrated solution of rutin at a concentration of 100 mg / mL.

[0024](2) Configuration of enrichment medium: add the rutin solution prepared in step (1) to the modified MRS medium at a certain concentration, so that the final concentration of rutin is 1 mg / mL. The formulation of the improved lactic acid bacteria culture medium (improved MRS) is shown in Table 1 below:

[0025] Table 1: Modified Lactic Acid Bacteria Medium (Modified MRS) Formula

[0026] Element Dosage (g) Peptone 20.0 Beef Extract 10.0 Yeast extract 5.0 Diammonium hydrogen citrate [(NH 4 ) 2 HC 6 h 5 o 7 ] 4.0 Glucose (C 6 h 12 o 6 ·H...

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Abstract

The invention provides a method for promoting growth of probiotics by adding rutin in a culture medium. According to the method, a probiotics strain (lactobacillus rhamnosus) is inoculated into a rutin-added improved MRS lactic acid bacteria culture medium for fermentation, wherein the improved MRS lactic acid bacteria culture medium includes the following components in percentage by weight: 2% of glucose, 2% of peptone, 1% of a beef extract, 0.5% of a yeast extract, 0.5% of sodium acetate, 0.2% of dipotassium phosphate, 0.2% of diammonium hydrogen citrate, 0.025% of magnesium sulfate, 0.005% of manganese sulfate, 0.3% of tween-80, and the pH is 7.0; 1g-5g of rutin (with the impurity of 99%) is added into each liter of the fermentation culture medium. According to the method, the fermentation speed of probiotics in fermentation liquid and the viable count of probiotics can be remarkably increased; after carrying out fermentation growth on probiotics for 24 hours, the maximal viable count of obtained probiotics fermentation liquid can reach about 10<9>CFU / mL; added rutin has the effects of decreasing capillary fragility, improving microcirculation, resisting radiation and free radicals and the like, and the functional effect of a cultured product can be substantially improved.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for adding rutin to a culture medium to promote the growth of probiotics. Background technique [0002] Probiotics are a general term for living microorganisms that are colonized in the human intestinal tract and reproductive system, can produce definite health effects and improve the host’s micro-ecological balance, and play an important role in human health. They are widely used in the production of food, health products and pharmaceuticals middle. LGG (ATCC 53103) is a kind of Lactobacillus rhamnosus, which was isolated from healthy people by two American professors in the early 1980s. It is also one of the most researched probiotics in the world. A large number of in vitro experiments, animal experiments, human experiments and epidemiological investigations have fully proved the safety of LGG. In terms of functionality, LGG can significantly balance and improv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20C12R1/225
Inventor 于大海房学迅相宏宇谢秋宏杨吉雨
Owner 吉林省浦生泰生物技术有限责任公司
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