Saccharomyces cerevisiae and application thereof

A technology of Saccharomyces cerevisiae and loquat wine, which is applied in the field of microbial engineering, can solve the problems of loquat fruit aroma, impure flavor, lack of specificity of yeast strains, etc., and achieve the effect of obvious hanging cup, long shelf life and rich flavor

Active Publication Date: 2015-02-25
重庆市南岸区广阳镇回龙枇杷种植观光园
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current strains used for the production of fruit wine generally use active dry yeast for brewing wine, and some also add common grain koji for fermentation. The yeast strains used for production lack specificity, resulting in the loquat fruit that loquat wine should have. Fragrance and flavor are impure

Method used

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  • Saccharomyces cerevisiae and application thereof
  • Saccharomyces cerevisiae and application thereof
  • Saccharomyces cerevisiae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 Isolation and purification of loquat wild yeast

[0013] The fresh loquat fruit is crushed and multiplied and cultured for 48 hours under the condition of 28-30 DEG C to obtain a loquat wild yeast multiplication culture liquid with more individuals. Dilute the obtained proliferation culture solution with sterile distilled water according to seven concentration gradients of 10 times, 20 times, 40 times, 80 times, 160 times, 320 times and 640 times, and then smear them on the plate selection medium respectively (plate selection culture Base formula: 4°Bé fresh loquat pulp filtrate 24.5%, 4°Bé wort juice 24.5%, 4°Bé soybean juice 24.5%, 4°Bé potato juice 24.5%, agar 2%), the culture solution of each concentration gradient Do 4 flat plates, and use the "plate streaking method" to separate dozens of single-cell colonies of loquat wild yeast. According to the colony form, single-cell individual form, and growth of wild yeast unicellular colonies, five robust pure s...

Embodiment 2

[0014] Embodiment 2 Small-scale test of performance determination of loquat wild yeast pure strain

[0015] 1 Purpose of the test

[0016] The fermentation ability and wine production effect of five isolated wild loquat yeast strains were verified.

[0017] 2 Test materials and methods

[0018] 15 kg of fresh loquats, remove the core and rotten pulp and beat to obtain 11.52 kg of loquat slurry, which is divided into 6 test samples, and the amount of each test sample is 1.92 kg. Wherein 5 test samples are used in the 5 strains of loquat wild yeast in Example 1 as the experimental group, and the other 1 sample is used as the commercially available commodity "Anqi" wine high activity dry yeast (BV818) as the control. In each loquat slurry test sample, add 200 grams of sucrose, 11.1 grams of fresh bran yeast (the fresh bran yeast added in the experimental group is prepared by No. 1, 2, 3, 4, and No. 5 loquat wild yeast in embodiment 1 respectively Obtained, the fresh bran yeast...

Embodiment 3

[0022] Embodiment 3. The medium-scale test of the performance determination of loquat wild yeast pure strain

[0023] 1 Purpose of the test

[0024] The isolated loquat wild yeast carried out a medium-scale test on the basis of the small-scale test, so as to further verify the performance of the wild yeast (color, flavor, wine production capacity, etc.).

[0025] 2 Test materials and methods

[0026] Get the fresh bran yeast prepared by No. 1, 2, 3, 4, No. 5 loquat wild yeast pure strains in Example 2 and mix in equal proportions, get 160 grams; 37.5 kilograms of loquat slurry (50 kilograms of loquats are obtained) ; 4.1 kilograms of sucrose; fully mixed and fermented at room temperature for 25 days. At the same time, "Anqi" wine high activity dry yeast (BV818) was used as a control.

[0027] 3 test results

[0028] After 25 days of fermentation at normal temperature, 3.55 kilograms of 53-degree distilled liquor were obtained, with a yield of 7.1%, and the wine-producing a...

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Abstract

The invention relates to saccharomyces cerevisiae and an application thereof and belongs to the field of microbial engineering. The separated saccharomyces cerevisiae is named as Yu2.145 which is suitable for brewing loquat liquor. The saccharomyces cerevisiae Yu2.145 is preserved in CGMCC (China General Microbiological Culture Collection Center), wherein the address is #3, Yard 1, West Beichen Road, Chaoyang District, Beijing; the preservation date is 9th, October, 2014; and the preservation number is CGMCC No.9753.

Description

technical field [0001] The invention relates to loquat wine Saccharomyces cerevisiae and an application thereof, belonging to the field of microbial engineering. Background technique [0002] In the brewing industry, yeast absorbs glucose, fructose, mannose and other monosaccharides into the cells, and under anaerobic conditions, through the action of internal enzymes, the monosaccharides are decomposed into carbon dioxide and alcohol, which is fermentation. However, yeasts of different types and origins have different fermenting abilities for wine-making raw materials, and the content and taste of fermented alcohol are also very different. The current strains used for the production of fruit wine generally use active dry yeast for brewing wine, and some also add common grain koji for fermentation. The yeast strains used for production lack specificity, resulting in the loquat fruit that loquat wine should have. The aroma and flavor are not pure. Contents of the invention...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12G1/022C12G3/02C12C11/00C12R1/685
CPCC12C11/00C12G1/00C12G3/02C12N1/185C12R2001/865
Inventor 杨廷元
Owner 重庆市南岸区广阳镇回龙枇杷种植观光园
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