Automatic operation method, system and carrier of live cell vitrification freezing/thawing

A vitrification, automatic operation technology, applied in the fields of biochemical equipment and methods, animal cells, preservation of human or animal bodies, etc., can solve the problems of affecting the operation results, the inability to standardize the operation results, and the increased physical fitness requirements of operators. achieve the effect of avoiding operational errors

Inactive Publication Date: 2015-03-11
广州蓝日生物科技有限公司
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  • Abstract
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Problems solved by technology

[0007] The operator quickly transfers the egg cells in a large number of sequential droplets, which requires strict skills of the operator, and misoperation and operational errors cannot be avoided; in the droplet connection method, the liquid environment of the cells is not controlled, and the operator's skills are required It is also very strict, and the operation results cannot be standardized, which seriously affects the clinical application of egg cell freezing and recovery
If the success rate of egg cell freeze-thaw recovery cannot be clo...

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  • Automatic operation method, system and carrier of live cell vitrification freezing/thawing
  • Automatic operation method, system and carrier of live cell vitrification freezing/thawing
  • Automatic operation method, system and carrier of live cell vitrification freezing/thawing

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Embodiment Construction

[0047] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0048] refer to figure 1 , is a structural block diagram of an automatic operating device for vitrification / thawing of living cells in an embodiment of the present invention. The automatic operating system includes a central controller 9 , a carrier mechanical action device 4 , a liquid drop replacement operation device 3 , a freezing / resuscitating carrier 6 , an operating table 7 carrying the freezing / resuscitating carrier, and a freezing medium container 8 . in:...

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Abstract

The invention discloses a live cell vitrification freezing/thawing carrier. The carrier body is a transparent sheet with an arc-shaped internal surface, which is provided with an ultra-hydrophobic surface functional area and a hydrophilic surface functional area, wherein the periphery of the hydrophilic surface functional area is surrounded by the ultra-hydrophobic surface functional area, and the hydrophilic surface functional area is provided with droplet positioning labels. The invention further discloses an automatic operation device and an automatic operation method of live cell vitrification freezing/thawing.

Description

technical field [0001] The present invention mainly relates to the technical field of vitrification, in particular to an automatic operation method and system for vitrification / thawing of living cells, and a carrier for vitrification / thawing of living cells. Background technique [0002] In artificial assisted reproductive technology, the in vitro preservation of human fertility has been widely concerned. So far, the human sperm bank has been in normal operation for nearly 30 years. The vitrification, freezing and thawing and resuscitation technology of fertilized eggs began to be popularized in the early part of this century, but the in vitro preservation of human oocytes is limited by the operation method, and no substantial progress has been made. , worldwide, there is no human egg bank that has been put into stable operation and application. In terms of physiological sources, human eggs are the main object of in vitro preservation of human fertility, followed by sperm; ...

Claims

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Application Information

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IPC IPC(8): A01N1/02C12N5/07C12M1/00C12M1/36
Inventor 徐小杨
Owner 广州蓝日生物科技有限公司
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