Automatic operation method, system and carrier of live cell vitrification freezing/thawing

Abstract

The invention discloses a live cell vitrification freezing / thawing carrier. The carrier body is a transparent sheet with an arc-shaped internal surface, which is provided with an ultra-hydrophobic surface functional area and a hydrophilic surface functional area, wherein the periphery of the hydrophilic surface functional area is surrounded by the ultra-hydrophobic surface functional area, and the hydrophilic surface functional area is provided with droplet positioning labels. The invention further discloses an automatic operation device and an automatic operation method of live cell vitrification freezing / thawing.

Description

technical field [0001] The present invention mainly relates to the technical field of vitrification, in particular to an automatic operation method and system for vitrification / thawing of living cells, and a carrier for vitrification / thawing of living cells. Background technique [0002] In artificial assisted reproductive technology, the in vitro preservation of human fertility has been widely concerned. So far, the human sperm bank has been in normal operation for nearly 30 years. The vitrification, freezing and thawing and resuscitation technology of fertilized eggs began to be popularized in the early part of this century, but the in vitro preservation of human oocytes is limited by the operation method, and no substantial progress has been made. , worldwide, there is no human egg bank that has been put into stable operation and application. In terms of physiological sources, human eggs are the main object of in vitro preservation of human fertility, followed by sperm; ...

AI Technical Summary

Problems solved by technology

[0007] The operator quickly transfers the egg cells in a large number of sequential droplets, which requires strict skills of the operator, and misoperation and operational errors cannot be avoided; in the droplet connection method, the liquid environment of the cells is not controlled, and the operator's skills are required It is also very strict, and the operation results cannot be standardized, which seriously affects the clinical application of egg cell freezing and recovery

If the success rate of egg cell freeze-thaw recovery cannot be close to 95% or even closer to 100%, the practicability of this operation method will be questioned, because the source of egg cells is limited, and the number of egg cells obtained after one treatment cycle will not be too much. In some cases, only one usable egg cell can be obtained per operation (micro-stimulation program, natural cycle egg retrieval program, etc.); if the number of egg cells to be frozen is large, the physical requirements of the operator will increase in the continuous repeated operation process. Affect the result of the operation

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