Method for separating liposome-heavy metal compound

A liposome and heavy metal technology, applied in the field of environmental protection pollution treatment, can solve the problems of inability to separate liposome-heavy metal complexes from free heavy metal ions, high instrument cost, long centrifugation time, etc., and achieves significant environmental advantages and Economic advantages, low disposal costs, high recovery rates

Inactive Publication Date: 2015-11-25
BEIJING NORMAL UNIVERSITY
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

This method has high instrument cost, requires the use of ultracentrifuge and ultrafiltration membrane, and takes a long time to centrifuge.
In addition, this method will cause liposomes to rupture and liposomes with small particle sizes to pass through the ultrafiltration membrane and enter the free (unadsorbed) metal ion solution, resulting in experimental errors
Other traditional methods such as ordinary filter membrane, gravity sedimentation, and dialysis cannot achieve the separation of liposome-heavy metal complexes and free heavy metal ions

Method used

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  • Method for separating liposome-heavy metal compound
  • Method for separating liposome-heavy metal compound
  • Method for separating liposome-heavy metal compound

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1: the separation of liposome-mercury complex and free mercury ion

[0031] Liposomes are prepared by solvent evaporation method. Weigh 100mg of ePC, 40mg of cholesterol and 10mg of DPPG solid in a 100mL round bottom flask, dissolve in a mixture of 5mL of chloroform / methanol solution, and place the round bottom flask on a rotary evaporator for evaporation in a water bath Solvent, placed in a vacuum oven to dry for at least 1 hour. Add 5-10mL Tris-HCl buffer solution for hydration until all the lipids are completely detached from the bottle wall to form a uniform milky white suspension without obvious particles. The liposome suspension was passed 21 times through a polycarbonate membrane with a pore size of 800 nm. The concentration of the mercury metal ion stock solution is 500mg / L, take 0.1mL metal ion stock solution, add 0.4mL liposome suspension, and place the resulting mixed solution in a constant temperature shaking box at 25±2°C for 12 hours in the dar...

Embodiment 2

[0036] Embodiment 2: the separation of liposome-cadmium complex and free cadmium ion

[0037] The difference from Example 1 is that liposomes are used to adsorb cadmium ions, and this method is used to separate liposome-cadmium complexes from free cadmium ions.

Embodiment 3

[0038] Embodiment 3: the separation of liposome-nickel complex and free nickel ion

[0039] The difference from Example 1 is that liposomes are used to adsorb nickel ions, and this method is used to separate liposome-nickel complexes from free nickel ions.

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Abstract

The invention relates to a method for separating a liposome-heavy metal compound from free heavy metal ions, which belongs to the technical fields of environmental protection and pollution treatment. The method mainly comprises the following steps: fully swelling a sephadex particle, uniformly filling a needle cylinder of a syringe with the swollen sephadex particle and balancing a cylinder with a buffer; adsorbing heavy metal ions by using size-stabilized liposome, adding a small amount of a sample into the cylinder drop by drop after adsorption equilibrium is realized, carrying out slow centrifugation so as to allow the sample to enter the cylinder, eluting the cylinder with an equivalent amount of the buffer a plurality of times, then carrying out centrifugation and collecting eluted components each time; and digesting the liposome, allowing a volume to be constant and determining the contents of elemental phosphorus and heavy metal elements. The method has the advantages of good separation effect, high efficiency in operation and low treating cost and is a separation method capable of overcoming practical problems and having environmental advantages.

Description

technical field [0001] The invention belongs to the technical field of environmental protection and pollution treatment, and relates to a technical method for separating liposome-heavy metal complexes from free heavy metal ions. Background technique [0002] In recent years, with the rapid development of human society, industrial economy and heavy metal pollution worldwide, the situation of heavy metal pollution has continued to intensify. Heavy metal pollution incidents frequently broke out in my country. Heavy metal ions can enter the human body through various channels and endanger human health. After the positively charged heavy metal ions enter the organism, the biomembrane is an important site for their action. Due to the complex composition of natural biological membranes, liposomes are usually used as a model to simulate biological membranes. [0003] Due to the similar structure and composition to natural biological membranes, liposomes are used in the field of e...

Claims

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Application Information

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IPC IPC(8): B01D15/08G01N1/28
Inventor 刘新会于艳军侯静梁宝翠王娟赵胜男
Owner BEIJING NORMAL UNIVERSITY
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