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A kind of mussel steroid active component extract and its preparation method and application

A technology of active components and extracts, applied in the field of mussel steroid active component extracts and its preparation, to achieve a strong anti-aging effect

Active Publication Date: 2017-01-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the anti-aging activity of mussels at present.

Method used

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  • A kind of mussel steroid active component extract and its preparation method and application
  • A kind of mussel steroid active component extract and its preparation method and application
  • A kind of mussel steroid active component extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Preparation of Mussel Steroid Active Component Extract

[0056] The preparation method of the mussel steroid active component extract of the present embodiment comprises the following steps:

[0057] (1) Put 179g of dried mussels in 1L of methanol (industrial grade), and extract at room temperature for 3 days (shaking); after concentrated by suction filtration, methanol extract is obtained.

[0058] (2) Extract and separate with 80% aqueous methanol and n-hexane, let stand overnight, concentrate the n-hexane layer under reduced pressure and spin dry, and obtain 4 g of crude n-hexane layer.

[0059] (3) Separating the crude sample of the n-hexane layer with a silica gel open column (200-300 mesh), using n-hexane: ethyl acetate solvent system as eluent, according to the volume ratio of n-hexane: ethyl acetate=99:1 , 98:2, 97:3, 96:4, 90:10, 0:100 were eluted in sequence, and the fraction eluted with a volume ratio of 90:10 was taken.

[0060] (4) Purify the fr...

Embodiment 2

[0069] Example 2 Anti-aging Activity Analysis of Mussel Steroid Active Component Extract

[0070] The biological models currently used for anti-aging research mainly include mice, nematodes, fruit flies and yeast. In this example, Saccharomyces cerevisiae was selected as the active system for anti-aging research, because yeast is a single-celled eukaryote with a short life cycle, and its complete genome data has been obtained, and it is currently a commonly used aging model organism. At the same time, resveratrol was used as a positive control. Resveratrol is a well-known small molecular compound that shows anti-aging effects on various animal models.

[0071] The analytical method includes the following steps:

[0072] (1) Take out the K6001 yeast strain from the -30°C refrigerator, wash it three times with PBS, 5ml each time, and remove the glycerol therein. Finally, 1 ml of PBS was added, blown to make it suspended, and then added to 5 ml of liquid medium (1% yeast powder...

Embodiment 3

[0080] Example 3 Anti-aging Activity Analysis of Mussel Steroid Active Component Extracts with Different Compositions

[0081] Test the influence of the mussel steroid active component extracts of different compositions on the yeast replicative lifespan in the test table 1, the analysis method is the same as embodiment 2, and the analysis results are shown in figure 2 .

[0082] Table 1 Composition of three kinds of mussel steroidal active components extracts

[0083]

[0084] Depend on figure 2 Visible, the average lifespan of negative control group yeast is 8.1 ± 0.43; Positive control is 10.6 ± 0.52***; SF (the mussel steroid active component extract that is embodiment 1 obtains) (0.1 μ g / ml) is 10.4 ±0.51***; Mixture 1 (0.07 μg / mL) is 10.5±0.53***; Mixture 2 (0.07 μg / mL) is 10.0±0.54** (**p<0.01 and ***p<0.001 indicate There is a significant difference).

[0085] right figure 2 The analysis of the results found that when the four sterol compounds were mixed in t...

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Abstract

The invention discloses a mussel steroid active component extract, a preparation method and applications thereof. The mussel steroid active component extract comprises the following components in percentage by weight: 25 to 39% of cholesterol, 19 to 27% of brassicasterol, 12 to 25% of (22E,24S)-ergosterol-5,22-diene-3beta-ol, and 12 to 25% of 24-methylene cholesterol. In a yeast aging model of K6001 beer yeast cells, the provided mussel steroid active component extract can prominently prolong the replicative life span of yeast cells, and the aging-resistant activity of the compound is higher than those of single cholesterol, brassicasterol, crinosterol, and 24-methylene cholesterol. The invention has an important meaning for the basic researches on preventing aging and development of novel drugs for treating aging related diseases.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a mussel steroid active component extract, a preparation method and application thereof. Background technique [0002] According to the latest statistics, the world's population aged 60 and above reached 814 million in 2013, and this number is expected to exceed 2 billion by 2050. The world has entered an aging society, and the accompanying diseases associated with aging have become increasingly prominent problems. Neurodegenerative diseases, including Alzheimer's disease, are among the world's most serious public health problems. In China alone, the number of patients with Alzheimer's disease has increased from 1.93 million in 1990 to 5.69 million in 2010. In the field of medicine, finding drugs for anti-aging and treating neurodegenerative diseases has become an urgent problem to be solved. [0003] For example, the Chinese patent document with the publication n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/575A61P39/00C07J9/00
CPCA23V2002/00A61K31/575A23V2200/302A23V2250/2136
Inventor 戚建华孙玉娟向兰
Owner ZHEJIANG UNIV
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