Device and method for culturing marine nitrite-dependent anaerobic methane oxidation microbes

Abstract

The invention discloses a device and method for culturing marine nitrite-dependent anaerobic methane oxidation microbes. The device comprises a reactor system and a controller system, wherein the reactor system comprises a reaction tank and a low-temperature water tank; a reaction tank cover plate is arranged at the upper part of the reaction tank; a stirring shaft is arranged in the middle of the reaction tank cover plate; the upper end of the stirring shaft is connected with a stirring motor, and the lower end of the stirring shaft is connected with a stirring paddle; a liquid sampling pipe, a gas samping opening, a gas intake pipe, a gas discharge pipe, a temperature probe, a DO (Dissolved Oxygen) probe, a pH probe, an electrical conductivity probe and a pressure probe are arranged on the reaction tank cover plate; the low-temperature water tank is mainly composed of a compressor, a condenser pipe and a circulating pump; a temperature probe and a liquid level probe are arranged on the side wall of the low-temperature water tank. The device disclosed by the invention can provide an anaerobic, low-temperature, high-pressure and high-salinity environment necessary for growth to the marine nitrite-dependent anaerobic methane oxidation microbes; the various probes and the controller system are used for allowing the device to be stably and efficiently operated, so as to implement fast cultivation of the marine nitrite-dependent anaerobic methane oxidation microbes.

Description

technical field [0001] The invention relates to a marine nitrite type methane anaerobic oxidation microorganism culture device and method. Background technique [0002] As an important energy source, methane plays an important role in human production and life. At the same time, methane is the most abundant hydrocarbon in the atmosphere, and its contribution to global warming is second only to CO 2 , the current "contribution rate" to global warming reaches 15%, and the greenhouse effect it causes is equivalent to mole CO 2 20 to 30 times of that. It is reported that 85% of the world's annual methane production and 60% of its consumption are based on the action of microorganisms. Anaerobic oxidation of methane by microorganisms can make most of the methane gas (more than 90%) be consumed in large quantities before entering the atmosphere. Therefore, anaerobic methane oxidation plays a role that cannot be ignored in the global methane emission control process, and it can ...

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the deficiencies of existing culture devices such as lack of pressure resistance, instability, poor robustness, poor sealing, lack of alarm system, etc., and provide a marine nitrite type methane anaerobic oxidation microbial culture device and method

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