Device and method for culturing marine nitrite-dependent anaerobic methane oxidation microbes

A technology for microbial cultivation and nitrite, which is applied in the field of marine nitrite-type methane anaerobic oxidation microbial cultivation device, can solve the problems of the cultivation device not being able to withstand pressure, lack of alarm system, poor robustness, etc., to increase ease of use , increase the integration, the effect of compact device

Inactive Publication Date: 2015-03-11
ZHEJIANG UNIV
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Problems solved by technology

[0007] The purpose of the present invention is to overcome the deficiencies of existing culture devices such as lack of pressure resistance, instability, p

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  • Device and method for culturing marine nitrite-dependent anaerobic methane oxidation microbes

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Embodiment Construction

[0013] like figure 1 As shown, the marine nitrite type methane anaerobic oxidation microbial cultivation device includes a reactor system 1, a controller system 2, and the reactor system 1 includes a reaction tank 3, a low-temperature water tank 4, a reaction tank cover 5, a stirring motor 6, a stirring Shaft 7, Stirring paddle 8, Reaction tank temperature probe 9, Reaction tank DO probe 10, Reaction tank pH probe 11, Reaction tank conductivity probe 12, Head air pressure probe 13, Liquid sampling tube 14, Gas sampling port 15, Air outlet tube 16. Air outlet valve 17, air inlet pipe 18, air inlet valve 19, compressor 20, condenser pipe 21, water tank liquid level probe 22, water tank temperature probe 23, water tank circulation pump 24, water tank water inlet 25, cork 26, water tank drainage Pipe 27, water tank drain valve 28, sampling needle 29; Reaction tank 3 is immersed in the water bath of low-temperature water tank 4, and reaction tank 3 upper end is reaction tank cover ...

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Abstract

The invention discloses a device and method for culturing marine nitrite-dependent anaerobic methane oxidation microbes. The device comprises a reactor system and a controller system, wherein the reactor system comprises a reaction tank and a low-temperature water tank; a reaction tank cover plate is arranged at the upper part of the reaction tank; a stirring shaft is arranged in the middle of the reaction tank cover plate; the upper end of the stirring shaft is connected with a stirring motor, and the lower end of the stirring shaft is connected with a stirring paddle; a liquid sampling pipe, a gas samping opening, a gas intake pipe, a gas discharge pipe, a temperature probe, a DO (Dissolved Oxygen) probe, a pH probe, an electrical conductivity probe and a pressure probe are arranged on the reaction tank cover plate; the low-temperature water tank is mainly composed of a compressor, a condenser pipe and a circulating pump; a temperature probe and a liquid level probe are arranged on the side wall of the low-temperature water tank. The device disclosed by the invention can provide an anaerobic, low-temperature, high-pressure and high-salinity environment necessary for growth to the marine nitrite-dependent anaerobic methane oxidation microbes; the various probes and the controller system are used for allowing the device to be stably and efficiently operated, so as to implement fast cultivation of the marine nitrite-dependent anaerobic methane oxidation microbes.

Description

technical field [0001] The invention relates to a marine nitrite type methane anaerobic oxidation microorganism culture device and method. Background technique [0002] As an important energy source, methane plays an important role in human production and life. At the same time, methane is the most abundant hydrocarbon in the atmosphere, and its contribution to global warming is second only to CO 2 , the current "contribution rate" to global warming reaches 15%, and the greenhouse effect it causes is equivalent to mole CO 2 20 to 30 times of that. It is reported that 85% of the world's annual methane production and 60% of its consumption are based on the action of microorganisms. Anaerobic oxidation of methane by microorganisms can make most of the methane gas (more than 90%) be consumed in large quantities before entering the atmosphere. Therefore, anaerobic methane oxidation plays a role that cannot be ignored in the global methane emission control process, and it can ...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/04C12M1/02
CPCC12M23/38C12M27/02C12M41/12C12M41/22C12M41/26C12M41/32C12M41/34C12M41/40C12M41/44
Inventor 胡宝兰何崭飞叶天强楼莉萍徐新华郑平
Owner ZHEJIANG UNIV
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