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Portable type micro-droplet generator based on self-powered vacuum micro pump

A technology of micro droplets and generators, which is applied in the directions of containers, laboratory utensils, chemical instruments and methods for laboratory use, can solve problems such as being unsuitable for portable analysis and detection, increase dead volume, etc., and achieve simple and easy assembly. Manipulate, avoid effects of flow connections

Active Publication Date: 2015-03-18
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods can generate highly monodisperse droplets in a high-throughput and controllable manner, but are limited by the need for external energy supply equipment, which is not suitable for portable analysis and detection
Relying on the static pressure of the liquid column to drive the generation of micro-droplets, although the problem of external energy supply is solved, the complicated pipeline connection significantly increases the dead volume of the system

Method used

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  • Portable type micro-droplet generator based on self-powered vacuum micro pump
  • Portable type micro-droplet generator based on self-powered vacuum micro pump

Examples

Experimental program
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Embodiment

[0022] The production of the microstructure on the cover sheet 1 with microstructure is as follows: first use photo-etching technology to make SU-8 template, then cast polydimethylsiloxane on the template, polymerize in an oven at 80°C for 2 hours, and finally The polymer imprinted with the microstructure is peeled from the mold. The flat substrate 2 is flat glass, and the cover sheet 1 and the flat substrate 2 with microstructures are treated with oxygen plasma and sealed together to form a microfluidic channel. Place the sealed chip in an oven at 80°C overnight to restore the hydrophobicity of the polydimethylsiloxane. Place the micro-droplet generator in a vacuum desiccator for degassing before use, and then quickly add the water phase solution and oil phase solution drops to the micro-droplet generator from the water phase solution inlet 3 and the oil phase solution inlet 4 respectively. Inside. Under the action of the negative pressure in the liquid microchannel 8 and th...

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Abstract

The invention relates to equipment of preparing micro-droplets and specifically relates to a portable type micro-droplet generator based on a self-powered vacuum micro pump. The portable type micro-droplet generator comprises a cover plate with a micro structure and a slab substrate which are arranged up and down, wherein the cover plate with the micro structure is in sealing connection to the slab substrate; an absorption room, a control room, a liquid micro channel and a gas micro channel are formed between the cover plate with the micro structure and the slab substrate; a water-phase solution inlet and an oil-phase solution inlet are formed in one end of the liquid micro channel, the other end of the liquid micro channel is connected to the absorption room, and the control room is connected to the absorption room by virtue of the gas micro channel, wherein the micro-droplet generator is used for forming a vacuum negative pressure environment in the liquid micro channel or the absorption room by virtue of vacuum degassing to provide driving force for generating micro-droplets, and switching a closing state and an opening sate of the micro-droplet generator by virtue of the control room to realize generation and stop of the micro-droplets. The portable type micro-droplet generator based on the self-powered vacuum micro pump disclosed by the invention has the characteristics of being simple to assemble, easy to operate and portable, and displays the great application potential in site real-time analysis.

Description

Technical field [0001] The invention relates to a device for preparing micro-droplets, specifically a portable micro-droplet generator based on a self-powered vacuum micro pump. [0002] The vacuum microenvironment formed by the degassed polydimethylsiloxane in the closed microchannel is used as the driving force to construct a self-powered, portable microdroplet generator. Background technique [0003] Micro-droplets are formed under the drive of external force and interfacial tension by using the immiscibility of water and oil. The traditional micro-droplet preparation method relies on strong stirring, shaking, or repeated pressing of pre-formed droplets through the membrane pores. These methods have disadvantages such as uncontrolled droplet size and poor monodispersity. In recent years, a new method for preparing micro-droplets using a microfluidic system has been proposed. It can not only prepare highly monodisperse droplets (the variation of droplet size is generally less ...

Claims

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Application Information

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IPC IPC(8): B01L3/00
Inventor 李春宇马波徐健
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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