A kind of acrocarp tissue culture method

Abstract

The invention provides a tissue culture method of acrocarpus fraxinifolius. The tissue culture method comprises the following steps: step 1. selecting an explant; step 2. treating the explant; step 3. inoculating; step 4. producing an induction culture medium; step 5. producing a subculture medium; and step 6. culturing a strong seedling, wherein the production formula of the induction culture medium comprises 1mg / L of MS+6BA, 0.2mg / L of NAA, 30g of white sugar and 6.5g of agar, and the production and selection formula of the subculture medium comprises 0.6mg / L of MS+6BA, 0.2mg / L of NAA, 30g of white sugar and 6.5g of agar. According to the vegetative rapid reproduction method of acrocarpus fraxinifolius, three different culture mediums are used for culturing acrocarpus fraxinifolius, and plants with ordered appearance can be reproduced in a large scale in relatively short time, and the variation of the regenerated plants is low; a regeneration system is very efficient; and the average cost of a single regenerated seedling is extremely low, the large-batch efficient and rapid reproduction of acrocarpus fraxinifolius is facilitated, so that the tissue culture method is used for industrial production.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for tissue culture of acrocarp tree. Background technique [0002] The top fruit tree grows fast, is a positive tree species, deciduous or semi-deciduous large tree, tall and straight. According to the data, the top fruit tree is particularly fast-growing in the early stage, has a long life span and slow decline, and can cultivate large-diameter timber. The germination power is very strong, and the plants can germinate after cutting. It can be used for greening, windbreak and soil fixation and timber. Its wood texture is straight, resistant to water and humidity, hard and tough, suitable for construction, furniture, etc., especially suitable for making utensils resistant to water and humidity; [0003] In the prior art, there is generally no one or several suitable culture mediums for induction of the rapid propagation of apical fruit trees, resulting in the fo...

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Problems solved by technology

[0003] In the prior art, there is generally no one or several suitable culture mediums for induction of the rapid propagation of apical fruit trees, resulting in the following adverse situations in the rapid propagation: the growth of the plants is irregular, and the plant variation rate is high, which in turn affects the growth rate of the later stage. Planting brings more trouble