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Method for preparing sugarcane tail silage by aspergillus niger, candida tropicalis, bacillus subtilis and lactobacillus plantarum

A technology of Candida tropicalis and Bacillus subtilis is applied in the preservation method, application, animal feed and other directions of animal feed raw materials, which can solve problems such as no reports and achieve the effect of improving quality

Inactive Publication Date: 2015-03-25
GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, so far, the literature on the application of Aspergillus niger, Candida tropicalis, Bacillus subtilis and Lactobacillus plantarum to silage fresh sugarcane tails has not been reported.

Method used

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  • Method for preparing sugarcane tail silage by aspergillus niger, candida tropicalis, bacillus subtilis and lactobacillus plantarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for preparing sugarcane tail silage with Aspergillus niger, the operation steps are as follows:

[0027] 1. Collection of silage raw materials:

[0028] Fresh sugarcane tails after sugarcane harvesting are collected as raw material.

[0029] 2. Chopped:

[0030] Cut the fresh sugarcane tails into 1cm-4cm lengths respectively.

[0031] 3. Spray Aspergillus niger bacterium liquid evenly on the fresh sugarcane tail raw material that is chopped through step (2), make the live bacteria content containing Aspergillus niger reach 2.3×10 in the fresh sugarcane tail 6 cfu / g.

[0032] 4. Filling, compacting, sealing:

[0033] The fresh sugarcane tail added with the Aspergillus niger liquid in step (3) is compacted layer by layer, filled into an airtight container with a density of more than 0.4kg / L, tightly covered, and sealed with a parafilm for storage.

[0034] 5. Silage fermentation:

[0035] Filling, compacting, and sealing the fresh sugarcane tails in step ...

Embodiment 2

[0041] The method for preparing sugarcane tail silage with Aspergillus niger+Candida tropicalis+Bacillus subtilis, the operation steps are as follows:

[0042] 1. Collection of silage raw materials:

[0043] Fresh sugarcane tails after sugarcane harvesting are collected as raw material.

[0044] 2. Chopped:

[0045] Cut the fresh sugarcane tails into 1cm-4cm lengths respectively.

[0046] 3. Spray the mixed preparation of Aspergillus niger + Candida tropicalis + Bacillus subtilis evenly on the fresh sugarcane tail raw material chopped through step (2), so that the number of live bacteria containing Aspergillus niger in the fresh sugarcane tail reaches 7.7 × 10 5 cfu / g, Candida tropicalis viable count reached 4×10 5 cfu / g, the number of viable Bacillus subtilis reached 1.4×10 7 cfu / g.

[0047] 4. Filling, compacting, sealing:

[0048] The fresh sugarcane tail added with the mixed preparation of Aspergillus niger + Candida tropicalis + Bacillus subtilis in step (3) is comp...

Embodiment 3

[0055] The method for preparing sugarcane tail silage with Aspergillus niger+Candida tropicalis+Bacillus subtilis+Lactobacillus plantarum, the operation steps are as follows:

[0056] 1. Collection of silage raw materials:

[0057] Fresh sugarcane tails after sugarcane harvesting are collected as raw material.

[0058] 2. Chopped:

[0059] Cut the fresh sugarcane tails into 1cm-4cm lengths respectively.

[0060] 3. Spray the mixed preparation of Aspergillus niger + Candida tropicalis + Bacillus subtilis + Lactobacillus plantarum evenly on the fresh sugarcane tail raw material chopped through step (2), so that the live bacteria content of Aspergillus niger is contained in the fresh sugarcane tail up to 5.75×10 5 cfu / g, Candida tropicalis viable count reached 3×10 5 cfu / g, the number of viable Bacillus subtilis reached 1.05×10 7 The content of cfu / g and the viable count of Lactobacillus plantarum reached 3×10 6 cfu / g.

[0061] 4. Filling, compacting, sealing:

[0062] Th...

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Abstract

The invention discloses a method for preparing sugarcane tail silage by aspergillus niger, candida tropicalis, bacillus subtilis and lactobacillus plantarum. The method comprises the following steps: shortly cutting mowed sugarcane tails to achieve the lengths being 1cm-4cm; uniformly spraying aspergillus niger, or aspergillus niger, candida tropicalis and bacillus subtilis, or a mixed preparation of aspergillus niger, candida tropicalis, bacillus subtilis and lactobacillus plantarum on fresh sugarcane tail raw materials so as to enable the contents of viable counts of aspergillus niger, candida tropicalis, bacillus subtilis and lactobacillus plantarum in the fresh sugarcane tails to reach 5.75*10<5>cfu / g-2.3*10<6>cfu / g, 3*10<5>cfu / g-4*10<5>cfu / g, 1.05*10<7>cfu / g-1.4*10<7>cfu / g and 3*10<6>cfu / g respectively; uniformly mixing; filling, compacting and sealing, wherein the density is 0.4kg / L; and preserving the product at room temperature for 40 days to prepare the sugarcane tail silage. According to the method disclosed by the invention, the problem that the palatability is poor when sugarcanes are directly used for feeding ruminants is solved, the content of lactic acid in the sugarcane tail silage is improved, and the content of acetic acid in the sugarcane tail silage is not improved.

Description

technical field [0001] The invention belongs to the technical field of silage preparation, in particular to a method for preparing sugarcane tail silage by using Aspergillus niger, Candida tropicalis, Bacillus subtilis and Lactobacillus plantarum. Background technique [0002] The harvest of sugarcane is seasonal. According to reports, the output of Guangxi alone in the 2012-2013 crushing season exceeded 75 million tons, and the output of sugarcane tail accounted for about 1 / 4 of the sugarcane output, which was 18.75 million tons. The problems encountered by sugarcane tails as forage grass for ruminants are that the palatability of direct application of sugarcane tails is poor, and the second is that the harvest season of sugarcane tails coincides with winter, and it is not easy to make dry forage grass. Therefore, can these sugarcane tails Effective treatment and long-term preservation are of great significance to the development of animal husbandry. Silage production is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K3/03
Inventor 邹彩霞夏中生韦升菊梁辛唐振华梁贤威李丽莉林波黄锋杨承剑唐庆凤黄加祥覃广胜莫乃国赵朝步潘玉红
Owner GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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