Method for degrading trichloroethylene by virtue of biological carbon immobilized microorganism co-metabolism

Abstract

The invention discloses a method for degrading trichloroethylene (TCE) by virtue of biological carbon immobilized microorganism co-metabolism, and belongs to the technical field of treatment with immobilized microorganisms. Biological carbon is a novel material, has an impact cascaded lamellar structure, has intensive micro-pores on the surface, and is relatively large in specific area and good in adsorption performance. The invention provides the method for degrading TCE in soil by virtue of co-metabolism by taking the biological carbon as a carrier, adopting an adsorption method to immobilize pseudomonas fluorescens and taking phenol as a substrate for the first time. By adopting the method, the degradation differences of TCE and phenol in two types of systems are compared, and the influence on the TCE degradation in an immobilization system by the adsorption time, pH value, TCE concentration, salinity and amount of added bacteria is inspected. Compared with a free system, the total removal rate of TCE by the immobilization system is improved by 15% and reaches 55%, and the tolerance of the immobilization system to bad environments is enhanced. The method disclosed by the invention adopts the adsorption method and takes the novel material namely biological carbon as the carrier to provide a new method for an immobilized microorganism technology.

Description

technical field [0001] The invention belongs to the technical field of immobilized microorganism treatment, and specifically relates to the application of immobilized Pseudomonas fluorescens by using biochar, using phenol as a growth substrate, and co-metabolizing and degrading trichlorethylene in microbial treatment of contaminated soil and environmental restoration. technical background [0002] Immobilized microorganism technology is a method of positioning free cells or enzymes in a limited area by chemical or physical means to keep them active and reusable. Immobilization technology has been widely used in industrial wastewater treatment due to its unique characteristics of high cell density, strong anti-toxicity and reusable use, and the effect is good. According to research, after microorganisms are immobilized, it can avoid the difficulty of separating microorganisms and the loss of microorganisms; it can also increase the density and physiological activity of microo...

AI Technical Summary

Problems solved by technology

The remediation technologies of TCE-contaminated soil and groundwater mainly include physical methods, chemical methods and microbial methods, among which physical methods only transfer pollutants to another phase, but do not remove pollutants; although chemical methods can completely decompose organic matter, they are easy to Leading to the destruction of the ecological environment, microbial methods have been widely concerned because of their high treatment efficiency, low cost, complete degradation and suitable for on-site treatment.

Images