A semi-continuous preparation method of camellia oleifera meal protein hypotensive peptide

A semi-continuous technology for antihypertensive peptides, which is applied in the field of semi-continuous preparation of antihypertensive peptides from camellia oleifera protein, can solve the problems of low yield of polypeptides, low antihypertensive activity, continuous production, etc., and achieve production costs Low, environmentally friendly effect

Inactive Publication Date: 2018-05-01
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the defects of low polypeptide yield, low blood pressure lowering activity and inability to realize continuous production in the existing preparation technology of blood pressure lowering peptides, and to provide a semi-continuous formula of oil tea meal protein blood pressure lowering peptide The preparation method, the obtained camellia oleifera meal protein blood pressure-lowering peptide has higher activity and higher yield

Method used

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  • A semi-continuous preparation method of camellia oleifera meal protein hypotensive peptide

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Embodiment 1

[0021] This embodiment includes the following steps:

[0022] (1) adopt the camellia oleifera meal protein of low degree of denaturation, its nitrogen solubility index (NSI) is 96.0%, the preparation of camellia oleifera meal protein mass concentration is the 3% camellia meal protein solution; The peristaltic pump continuously replenishes the camellia oleifera protein liquid into the reactor; the preparation method of the low-denaturation camellia oleifera protein: adopts cold pressing method to produce oil at 61°C, and extracts the camellia oleifera meal by alkali-dissolving and acid-precipitating method The protein was dried under vacuum (0.035MPa) at low temperature (38°C) to obtain camellia oleifera meal protein with low degree of denaturation; the degree of denaturation was characterized by Nitrogen Solubility Index (NSI);

[0023] (2) The camellia oleifera meal protein solution obtained in step (1) passes through reactors I, II, and III equipped with proteases I, II, and...

Embodiment 2

[0031] This embodiment includes the following steps:

[0032] (1) adopt the camellia oleifera meal protein of low degree of denaturation, its nitrogen solubility index (NSI) reaches 98.0%, the preparation of the camellia oleifera meal protein mass concentration is 3.5% the camellia oleifera meal protein solution; The peristaltic pump continuously replenishes the camellia oleifera protein liquid into the reactor; the preparation method of the low-denaturation camellia oleifera protein: adopts the cold pressing method to produce oil at 62°C, and extracts the camellia oleifera meal by alkali-dissolving and acid-precipitating The protein is dried under vacuum (0.032MPa) at low temperature (39°C) to obtain the camellia oleifera meal protein with low denaturation degree; the degree of denaturation is characterized by Nitrogen Solubility Index (NSI);

[0033] (2) The camellia oleifera meal protein solution obtained in step (1) passes through reactors I, II, and III equipped with prot...

Embodiment 3

[0041] This embodiment includes the following steps:

[0042] (1) adopt the camellia oleifera meal protein of low degree of denaturation, its nitrogen solubility index (NSI) reaches 96.5%, the preparation of camellia oleifera meal protein mass concentration is the 4.0% camellia meal protein solution; The peristaltic pump continuously replenishes the camellia oleifera protein liquid into the reactor; the preparation method of the low-denaturation camellia oleifera protein: adopts cold pressing method to produce oil at 63°C, and extracts the camellia oleifera meal by alkali-dissolving and acid-precipitating method The protein is dried under vacuum (0.030MPa) at low temperature (40°C) to obtain the camellia oleifera meal protein with low denaturation degree; the degree of denaturation is characterized by Nitrogen Solubility Index (NSI);

[0043] (2) The camellia oleifera meal protein solution obtained in step (1) passes through reactors I, II, and III equipped with proteases I, I...

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Abstract

The invention relates to a semi-continuous preparation method of an antihypertensive peptide of an oil-tea meal protein. The preparation method comprises the following steps: (1) preparing an oil-tea meal protein liquid with mass concentration of oil-tea meal proteins being 2-6%; feeding the oil-tea meal protein liquid into a charging tank and continuously supplementing the oil-tea meal protein liquid to a reactor by using a peristaltic pump; (2) enabling the oil-tea meal protein liquid obtained to sequentially pass through reactors I, II and III filled with protease, wherein each reactor region is provided with a pH value induction probe and an automatic pH value adjustor; (3) carrying out ultra-filtration; (4) feeding the filtrate obtained by ultra-filtration into a collecting tank; (5) desalting; and (6) drying to obtain the antihypertensive peptide. By adopting the preparation method provided by the invention, the antihypertensive peptide of the oil-tea meal protein can be semi-continuously prepared and the ash content of the prepared antihypertensive peptide of the oil-tea meal protein is smaller than 1.0%, the protein recovery rate is 93.0% and the ACE inhibition rate is greater than 94.0%. The antihypertensive peptide of the oil-tea meal protein has a remarkable blood-pressure-reducing effect on a rat with primary hypertension.

Description

technical field [0001] The invention relates to a semi-continuous preparation method of cake protein blood pressure-lowering peptide, in particular to a semi-continuous preparation method of camellia oleifera meal protein blood pressure-lowering peptide. Background technique [0002] Hypertension (systolic blood pressure greater than 140 mmHg and / or diastolic blood pressure greater than 90 mmHg) is a worldwide cardiovascular disease that is highly associated with myocardial infarction, stroke, atherosclerosis, coronary heart disease, and stroke It is extremely harmful to the health of the body. In the regulation of peripheral vascular blood pressure, ACE (EC 3.4.15.1) plays an important role, and more and more evidences show that it is the main risk factor induced by cardiovascular disease. ACE is a dipeptide carboxyl peptidase, which is not only related to the regulation of blood pressure, but also related to cardiovascular function. In the renin-angiotensin system, by cl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34A61P9/12
Inventor 龚吉军黄卫文王挥唐静卢锟
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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