A kind of preparation method of glucosinolate and its metabolite benzyl isothiocyanate

A technology of benzyl isothiocyanate and glucosinolate, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problem of low yield, environmental pollution, extraction and separation of benzyl isothiocyanate No problems such as reports have been reported, achieving the effects of low extraction temperature, avoidance of thermal damage, and safe extraction process

Active Publication Date: 2017-04-19
丽江英煌集生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, glucosinolates are mostly extracted with organic solvents, then acid-treated, and finally refined by macroporous resin adsorption or countercurrent chromatography. A large amount of acidic wastewater is generated during the production process, which is likely to cause great environmental pollution and low yield; benzyl isothiocyanate The extraction and separation of esters has not been reported

Method used

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  • A kind of preparation method of glucosinolate and its metabolite benzyl isothiocyanate
  • A kind of preparation method of glucosinolate and its metabolite benzyl isothiocyanate
  • A kind of preparation method of glucosinolate and its metabolite benzyl isothiocyanate

Examples

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Embodiment 1

[0038] A preparation method of glucosinolate and its metabolite benzyl isothiocyanate, the steps and conditions thereof are as follows:

[0039] Step 1: Tertiary Supercritical CO 2 extraction

[0040] 10.00kg of maca (containing 3.54% benzyl isothiocyanate and 4.05% glucosinolate) was inactivated by microwaves, crushed to 100 mesh, and three-stage series supercritical CO 2 extraction, such as figure 2 As shown, three series-connected extraction kettle 1, extraction kettle 2, and extraction kettle 3 are connected to two series-connected separation kettle 1, separation kettle 2, CO 2 The flow rate is 300L / h, the extraction pressure is 16.0MPa, the extraction temperature is 65.0°C, the primary separation pressure is 6.5Mpa, the primary separation temperature is 50.0°C, the secondary separation pressure is 4.5Mpa, and the secondary separation temperature is 50.0°C. After extracting for 100.0min, release the extract from separation tanks 1 and 2; use three-stage series supercri...

Embodiment 2

[0050] A preparation method of glucosinolate and its metabolite benzyl isothiocyanate, except for the following differences, other steps and process conditions thereof are the same as in Example 1:

[0051] (1) Step 1: CO 2 The flow rate is 240L / h, the extraction pressure is 20.0MPa, the extraction temperature is 55.0°C, the extraction time is 75.0min, the primary separation pressure is 7.5Mpa, the primary separation temperature is 40.0°C, the secondary separation pressure is 5.0Mpa, and the secondary separation pressure is 5.0Mpa. The separation temperature was 40.0°C, and 1.19 kg of dehydrated extract was obtained.

[0052] (2) Step 2: The quality of adding absolute ethanol is 1.05kg, stirring once every 30.0min with intermittent stirring, 3.0min each time, the cooling rate of intermittent stirring is 3.0°C / h, the static cooling analysis temperature is 0.0°C, and the static cooling The analysis time is 12.0h.

[0053] (3) Step 3: 60.0 g of immobilized enzyme was added, the r...

Embodiment 3

[0056] A preparation method of glucosinolate and its metabolite benzyl isothiocyanate, except for the following differences, other steps and process conditions thereof are the same as in Example 1:

[0057] (1) Step 1: CO 2 The flow rate is 180L / h, the extraction pressure is 24.0MPa, the extraction temperature is 46.0°C, the extraction time is 50.0min, the primary separation pressure is 8.5Mpa, the primary separation temperature is 30.0°C, the secondary separation pressure is 6.0Mpa, and the secondary separation pressure is 6.0Mpa. The separation temperature was 30.0°C, and 1.25 kg of dehydrated extract was obtained.

[0058] (2) Step 2: The quality of adding absolute ethanol is 1.20kg, stirring once every 60.0min with intermittent stirring, 5.0min each time, the cooling rate of intermittent stirring is 2.0°C / h, the static cooling analysis temperature is 5.0°C, and the static cooling The analysis time is 18.0h.

[0059] (3) Step 3: 90.0 g of immobilized enzyme was added, the...

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Abstract

The invention discloses a preparation method of glucosinolate and benzyl isothiocyanate as a metabolite of glucosinolate. The preparation method comprises the following steps: firstly carrying out supercritical CO2 extraction on enzyme-deactivated Maca powder to extract fat-soluble components, removing impurities such as sterol by a cold precipitation method, carrying out ethyl esterification on the grease in the extract by an enzyme-catalyzed alcoholysis method and finally carrying out two-stage molecular distillation to obtain benzyl isothiocyanate and glucosinolate. Since ethanol is only used as a solvent, the preparation method is safe, non-toxic, free of pollution and simple in process and the scale production is easily achieved.

Description

technical field [0001] The present invention relates to a kind of preparation method of glucosinolate and its metabolite benzyl isothiocyanate, specifically, the maca powder after deactivating the enzyme first adopts supercritical CO 2 Extract the fat-soluble components, then remove impurities such as sterols by cold analysis, then use enzyme-catalyzed alcoholysis to esterify the oil in the extract, and finally obtain benzyl isothiocyanate and mustard oil through two-stage molecular distillation Glycosides. The invention belongs to the field of natural organic chemistry. Background technique [0002] Glucosinolate and its metabolite benzyl isothiocyanate are important components of maca, and they can be used as substitutes for chemically synthesized estrogen. Other studies have shown that glucosinolates and their isothiocyanates have anti-cancer effects. [0003] Benzylglucosinolate and m-methoxybenzylglucosinolate have a good preventive effect on many tumors, especially ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/14C07H1/08C07C331/24
CPCC07C331/24C07H1/08C07H15/14Y02P20/54
Inventor 木立忠郑武张之会
Owner 丽江英煌集生物工程有限公司
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