A method for analyzing the composition of humic substance-like functional groups with different molecular weights
A humic substance and functional group technology, which is applied in the direction of analyzing materials, measuring devices, and material separation, can solve the problems of large labor load, high sample demand, and inconvenient composition, so as to achieve less sample demand, reduce pollution, and sample volume little effect
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[0039] Organic matter extraction: Collect 5 samples at different composting stages, numbered S1, S2, S3, S4, S5 respectively, add ultrapure water, extract and shake for 16 hours, centrifuge the supernatant and pass through a filter membrane with a pore size of 0.45 μm , the filtrate was collected, a part was stored at 4°C, and a part was lyophilized into solid powder.
[0040] Infrared spectrum scanning: Mix the above five solid powder samples with spectrally pure KBr and press them into tablets, and scan the 4000-400cm -1 Infrared spectra in the range, and then convert its data to absorption spectrum mode, save 5 samples in a file in the order of S1-S5 in csv data format.
[0041] Adjust filtrate concentration: use ultrapure water to adjust the DOC concentration of 5 compost samples to make DOC=5mgL -1 ,spare.
[0042] Three-dimensional fluorescence spectrum scanning of the filtrate: adjust the above 5 to DOC=5mgL -1 The sample is subjected to three-dimensional fluorescenc...
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