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Method for detecting antique silk fabric

A detection method and technology of silk fabrics, applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems of low sensitivity, unrecognizable, unresolved, etc., and achieve the effect of simple operation, strong specificity, and avoiding interference

Inactive Publication Date: 2015-03-25
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as an organic polymer material, silk is buried in the tomb environment for a long time, and it will inevitably be degraded by factors such as oxygen, water, and microorganisms, so that the appearance is severely damaged and decayed, and it cannot be recognized by the naked eye.
Conventional detection methods include infrared spectrum analysis, amino acid analysis, liquid chromatography analysis, etc., but due to the high content of impurities in waste silk fabrics, it is difficult to analyze the spectrum, it takes a long time, the sensitivity is low, and the interference of other proteins cannot be ruled out. brought some difficulties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 concrete implementation steps are as follows:

[0018] A) Preparation of solution: PBS 7.4 buffer preparation: KCl 0.2g, KH 2 PO 4 0.27g, NaCl 8.0g, NaCl 2 HPO 4 1.42g, dissolved in 800ml of distilled water and adjusted to 1000ml, adjusted to pH 7.4; PBS 9.6 buffer solution preparation: Na 2 CO 3 0.15g, NaHCO 3 0.29g, dissolved in 800ml of distilled water and adjusted to 1000ml, adjust the pH to 9.6;

[0019] B) Dissolve 0.001g of silk fibroin powder and 0.01g of cultural relics in 10ml of PBS buffer solution with a pH of 9.6, mix and stir evenly, let stand, take 50μl of the supernatant and add them to the microplate to form a positive control and experimental control; take 50 μl of PBS buffer solution with a pH of 7.4 as a blank control; the negative control is no rabbit anti-silk fibroin primary antibody, and its coating solution is set to be the same as the positive control; then each negative control, positive control , each experimental grou...

Embodiment 2

[0028] Embodiment 2 concrete implementation steps are as follows:

[0029] A) Preparation of solution: PBS 7.4 buffer preparation: KCl 0.2g, KH 2 PO 4 0.27g, NaCl 8.0g, NaCl 2 HPO 4 1.42g, dissolved in 800ml of distilled water and adjusted to 1000ml, adjusted to pH 7.4; PBS 9.6 buffer solution preparation: Na 2 CO 3 0.15g, NaHCO 3 0.29g, dissolved in 800ml of distilled water and adjusted to 1000ml, adjust the pH to 9.6;

[0030] B) Dissolve 0.005g of silk fibroin powder and 0.05g of cultural relics in 500ml of PBS buffer solution with a pH of 9.6, mix and stir evenly, let stand, take 80μl of the supernatant and add them to the microtiter plate to form a positive control and experimental control; take 80 μl of PBS buffer solution with a pH of 7.4 as a blank control; the negative control is without the addition of rabbit anti-silk fibroin primary antibody, and its coating solution is set to be the same as the positive control; then each negative control, positive contr...

Embodiment 3

[0039] Embodiment 3 specific implementation steps are as follows:

[0040] A) Preparation of solution: PBS 7.4 buffer preparation: KCl 0.2g, KH 2 PO 4 0.27g, NaCl 8.0g, NaCl 2 HPO 4 1.42g, dissolved in 800ml of distilled water and adjusted to 1000ml, adjusted to pH 7.4; PBS 9.6 buffer solution preparation: Na 2 CO 3 0.15g, NaHCO 3 0.29g, dissolved in 800ml of distilled water and adjusted to 1000ml, adjust the pH to 9.6;

[0041] B) Dissolve 0.01g of silk fibroin powder and 0.1g of cultural relics in 1000ml of PBS buffer solution with a pH of 9.6, mix and stir evenly, let stand, take 120μl of the supernatant and add them to the microplate to form a positive control and experimental control; take 120 μl of PBS buffer solution with a pH of 7.4 as a blank control; the negative control is no rabbit anti-silk fibroin primary antibody, and its coating solution is set to be the same as the positive control; then each negative control, positive control , each experimental gr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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PUM

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Abstract

The invention discloses a method for detecting antique silk fabric. The method comprises the steps that an indirect ELISA method is used for detecting the silk peptide component of the silk fabric, namely silk fabric antique eluant wraps an elisa plate, and then rabbit anti silk fibroin antibodies are added to be combined with antigens to form an antigen-antibody compound; after washing, alkaline phosphatase enzyme label IgG(H+L) antibodies are added, an antibody-antigen-antibody compound is formed, a tetramethyl benzidine solution is added after washing, a substrate is catalyzed to be a colored product by enzymes, and qualitative analysis can be conducted according to the depth of color variation of the product. Due to the fact that the catalytic efficiency of the enzymes is very high, the immunoreaction result is indirectly acquired, and the test has high sensitivity.

Description

technical field [0001] The invention belongs to the detection field of ancient silk fabrics, and in particular relates to a detection method of ancient silk fabrics. Background technique [0002] Silk fabrics are woven from silk, which is the crystallization of the wisdom of the Chinese nation and a precious heritage of our country. However, as an organic polymer material, silk is buried in the tomb environment for a long time, and it will inevitably be degraded by factors such as oxygen, water, and microorganisms, so that the appearance is severely damaged and decayed, and it cannot be recognized by the naked eye. Conventional detection methods include infrared spectrum analysis, amino acid analysis, liquid chromatography analysis, etc., but due to the high content of impurities in waste silk fabrics, it is difficult to analyze the spectrum, it takes a long time, the sensitivity is low, and the interference of other proteins cannot be ruled out. posed certain difficulties....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Application Information

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IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/6803
Inventor 刘苗苗杨颖超王秉
Owner ZHEJIANG SCI-TECH UNIV
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