Seed-soaking reagent for promoting cistanche seed germination and preparation method of seed-soaking reagent

A technology of Cistanche deserticola and seed soaking liquid, applied in botany equipment and methods, seed and rhizome treatment, chemicals for biological control, etc., can solve the problems of limited improvement of germination rate, etc. harm, less environmental pollution

Active Publication Date: 2015-04-01
INST OF AFFORESTATION & DESERTIFICATION PREVENTION & CONTROL XINJIANG ACADEMY OF FOREST SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] But the germination of above-mentioned Cistanche seeds is all only processed from the inducti

Method used

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  • Seed-soaking reagent for promoting cistanche seed germination and preparation method of seed-soaking reagent
  • Seed-soaking reagent for promoting cistanche seed germination and preparation method of seed-soaking reagent
  • Seed-soaking reagent for promoting cistanche seed germination and preparation method of seed-soaking reagent

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1:

[0024] 1. Test materials:

[0025] In 2011, the desert Cistanche deserticola seeds (Cistanche deserticola Ma) were harvested in Jimsar County, and the seeds were classified by particle size, and high-quality desert Cistanche seeds with a diameter greater than 0.7mm were selected for use.

[0026] 2. Pharmacy and equipment:

[0027] NaClO, fluridone, gibberellin, acetone, 95% ethanol, etc.; the instrument is a standard sieve, XTZ-EA (45X) stereo microscope (with micrometer), biochemical incubator, 9cm petri dish, foil paper. Both fluridone and gibberellin were purchased by Xinjiang Furike Biotechnology Co., Ltd., with the product number of halidone 45511 and the product number of gibberellin wi78755.

[0028] 3. Seed disinfection:

[0029] Soak the seeds of Cistanche deserticola with 70% ethanol for 2 minutes, then disinfect with 2% sodium hypochlorite for 15 minutes, and finally rinse with distilled water 3 times.

[0030] 4. Seed germination test:

[0031] (1) Treatme...

Example Embodiment

[0046] Example 2:

[0047] 1. Test materials:

[0048] In 2012, Cistnche salsa (C.A.Mey.)G.Beck.) was harvested in Habahe County. The seeds were classified by particle size, and high-quality seeds of Cistanche salsa with a diameter of 0.55-0.7mm were selected for use.

[0049] 2. Pharmacy and equipment:

[0050] NaClO, fluridone, gibberellin, acetone, 95% ethanol, etc.; the instrument is a standard sieve, XTZ-EA (45X) stereo microscope (with micrometer), biochemical incubator, 9cm petri dish, foil paper. Both fluridone and gibberellin were purchased by Xinjiang Furike Biotechnology Co., Ltd., with the product number of halidone 45511 and the product number of gibberellin wi78755.

[0051] 3. Seed disinfection:

[0052] Soak the seeds of Cistanche cistanche with 70% ethanol for 2 minutes, then disinfect with 2% sodium hypochlorite for 15 minutes, and finally rinse with distilled water 3 times.

[0053] 4. Seed germination test:

[0054] (1) Treatment 1: Prepare fluridone with acetone (fir...

Example Embodiment

[0069] Example 3:

[0070] 1. Test materials:

[0071] In 2009, Cistanche tubulosa (schrenk) R. Wight was harvested in Hotan, and the seeds were sorted by particle size, and high-quality seeds of Cistanche tubulosa (schrenk) R. Wight with a diameter greater than 0.7 mm were selected for use.

[0072] 2. Pharmacy and equipment:

[0073] NaClO, fluridone, gibberellin, acetone, 95% ethanol, etc.; the instrument is a standard sieve, XTZ-EA (45X) stereo microscope (with micrometer), biochemical incubator, 9cm petri dish, foil paper. Both fluridone and gibberellin were purchased by Xinjiang Furike Biotechnology Co., Ltd., with the commodity number of fluridone and gibberellin being 45511 and wi78755.

[0074] 3. Seed disinfection:

[0075] Soak the seeds of Cistanche tubulosa in 70% ethanol for 2 minutes, then sterilize them with 1% sodium hypochlorite for 10 minutes, and finally rinse with distilled water 3 times.

[0076] 4. Seed germination test:

[0077] (1) Treatment 1: Prepare fluridone...

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Abstract

The invention discloses a seed-soaking reagent for promoting cistanche seed germination. The seed-soaking reagent is prepared from a fluridone solution and a gibberellin solution in an equivalent volume mixing manner. The invention also provides a preparation method of the seed-soaking reagent. The seed-soaking reagent has the beneficial effects that the cistanche seeds are soaked with the seed germinating and soaking reagent for a certain period of time; relatively high germination rate can be reached within a relatively short period of time under an appropriate environmental condition; seed dormancy is broken; seed germination is promoted; the germination rate can be improved to over 75%; the problems of low germination rate and large sowing quantity in artificial cultivation of cistanche seeds are solved; a foundation is laid for artificial cultivation and inoculation of the cistanche by using the seed germinating and soaking reagent; due to the seed germinating and soaking reagent disclosed by the invention, a new index can be provided for quality determination of the cistanche seeds for commodity marketing; due to the seed-soaking reagent, the seed germination effect is improved; and the seed-soaking reagent is low in cost, light in environmental pollution, harmless to people and animals, and convenient to popularize and use.

Description

Technical field [0001] The present invention involves the field of medicinal plant breeding, and specifically involves a seedlings and preparation methods that promote the germination of Cistanche. Background technique [0002] There is a dormant phenomenon of Cistanche seeds. The start of the seed germination. In addition to the long -term cooked process, there must be some germinated stimulating signal from the host root system. [0003] In recent years, the artificial cultivation area of Cistanche has expanded year by year, but low germination rate and vaccination parasite rate is a key issue that restricted Cistanche output.The present invention artificially induced the desert cordon, salt raw Cistanche, and tube flowers of cistanason seeds from the seeds of the seeds and chemicals, to find the environmental conditions that are suitable for seed germination.The in vaccination rate has important theoretical value and practical significance. [0004] Sheng Jinhua and others stu...

Claims

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Application Information

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IPC IPC(8): A01N43/40A01C1/00A01P21/00A01N43/12
Inventor 刘永萍杨瑞鲁天平郭靖刘爱民莫双铭江晓珩张峰
Owner INST OF AFFORESTATION & DESERTIFICATION PREVENTION & CONTROL XINJIANG ACADEMY OF FOREST SCI
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