Ureter ligation induced non-human primate animal renal fibrosis model building method and application

Abstract

The invention discloses a ureter ligation induced non-human primate animal renal fibrosis model building method and application. The specific technical scheme includes that an experimental monkey is anaesthetized by anesthetics, surgical anesthesia is maintained, the abdomen of the experimental monkey is cut apart along a medioventral line after disinfection, an abdominal cavity is opened, an operation field is exposed, a unilateral ureter at a unilateral renal lower edge is ligated twice by a surgical silk thread and completely blocked, another kidney is not ligated and serves as a self-body for comparison, a wound is stitched in a layered manner after a surgical incision is disinfected, renal fibrosis indexes are measured by biological samples at different time points after ligation, or a left kidney and a right kidney are taken down and fixed in neutral buffered formalin, and formalin fixed kidney samples are subjected to tissue section and pathological examination. An animal model has application values which cannot be realized by an existing rodent and rabbit animal model, and the non-human primate animal model built by the method has wide application in related fields of fibrosis.

Description

technical field [0001] The invention relates to the establishment of an animal model of a disease, in particular to a method for establishing a renal fibrosis model of a non-human primate induced by ureteral ligation and its application. Background technique [0002] Renal fibrosis is a pathophysiological change characterized by abnormal deposition of extracellular matrix (ECM), glomerulosclerosis, and interstitial fibrosis, and is an end-stage manifestation of all renal diseases. The kidney is stimulated by various pathogenic factors such as trauma, infection, inflammation, blood circulation disorder, and immune response, resulting in cell damage and infiltration of macrophages in the renal parenchyma. Cytokines released by macrophages promote tubular apoptosis and fibroblast activation and proliferation, while tubular cell apoptosis and necrosis cause tubular necrosis and the formation of atubular glomeruli. This then causes changes in the phenotype of renal parenchymal c...

AI Technical Summary

Problems solved by technology

[0003] Currently, animal models of renal fibrosis are usually established on rodents and rabbit experimental animals. However, since these experimental animals are far from humans in terms of evolutionary relationship, kidney tissue structure and biochemistry, and renal fibrosis lesions, etc., The experimental data obtained as the research object has little significance for practical guidance

Practice has shown that experimental results generated using rodent and rabbit renal fibrosis models are difficult to verify in clinical trials, and the success rate of drug development based on this model data is also low

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