Fabrication method of fluorescence sensor based on graphite-like phase nitrogen carbide nanomaterial

Abstract

The invention provides a method for manufacturing a fluorescent sensor based on a graphite-like nitrogen carbide nano material. The fluorescent sensor is manufactured by combining the graphite-like nitrogen carbide nano material with an enzyme digestion circulation signal amplification technology. The fluorescent sensor comprises a graphite-like nitrogen carbide nano sheet (g-C3N4-NF), Ag<+>, a Nb. BsmI incision enzyme, probe genes and target genes (short fragments of Cerb B-2 related genes). The fluorescent sensor is manufactured through a circulation process of hybridization, enzyme digestion hydrolysis and re-hybridization, so that a target gene can release Ag<+> on a plurality of hairpin structure probe genes, free Ag<+> in the solution is increased, the fluorescent quenching degree of nitrogen carbide is enhanced and fluorescent signals are obviously weakened. High-sensitivity detection of target genes can be realized through the technology of amplifying circulation signals for multiple times.

Description

technical field [0001] The invention belongs to the field of preparation of fluorescence sensors, and in particular relates to a method for preparing a fluorescence sensor based on graphite-like phase nitrogen carbide nanomaterials. Background technique [0002] Breast cancer is one of the most common malignant tumors in women worldwide. Early diagnosis is the key to improve the cure rate of breast cancer. So far, the basic methods for breast cancer diagnosis mainly include mammography, breast B-ultrasound, and dynamic enhanced nuclear magnetic resonance. However, the above inspection methods may easily lead to missed diagnosis; or the operation is cumbersome, time-consuming, and the equipment is expensive, so it is difficult to apply to large-scale breast cancer screening. As the gold standard for the diagnosis of breast cancer—histopathological diagnosis based on needle biopsy is traumatic, which not only causes physical pain to the patient, but also causes anxiety and f...

AI Technical Summary

Problems solved by technology

After the discovery of this type of enzyme, it has attracted widespread attention of scientific researchers. In recent years, some scholars have used nicking endonuclease-assisted signal amplification technology for DNA detection, although this technology has effectively improved the detection efficiency. Sensitivity, but because these methods require the use of molecular beacon technology, there are disadvantages such as complicated operation and high price

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