Molecular-specific marker primers and methods for identifying southern yew seedlings
A technology of southern yew and labeled primers, which is applied in the field of rapid identification of southern yew, can solve the problems of difficult and accurate identification, and achieve the effect of high sensitivity and simple method
Active Publication Date: 2016-06-08
HANGZHOU NORMAL UNIVERSITY
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Problems solved by technology
Therefore, it is difficult to accurately identify the three similar species by PCR amplification with general ITS primers (ITS4 / ITS5) and ordinary electrophoresis
Method used
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Embodiment 1
[0027] 1. Extraction of the genomic DNA of the yew sample to be tested
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The invention relates to a molecular specificity labeled primer for identifying a T. chinensis seedling and a method. An upstream primer NHSF of the molecular specificity labeled primer for identifying T. chinensis and two similar species including T. madia and T. cuspidata is represented as SEQ ID NO.1, and downstream NHSR is represented as SEQ ID NO.2; and by means of the molecular specificity labeled primer MHSF / MHSR, the T. chinensis and the two similar species of the T. chinensis can be rapidly subjected to molecular identification with a conventional PCR (polymerase chain reaction) method. The method is simple, the PCR technology is adopted for detection, time is short, and the detection can be finished by half day; and the molecular specificity labeled primer and the method provide effective assistance for identification of the T. chinensis and the two similar species of the T. chinensis according to morphological characteristics.
Description
technical field [0001] The invention relates to a molecular specific marker primer for identifying the southern yew Taxuschinensis, and a method for rapidly identifying the southern yew by using the specific primer. Background technique [0002] Southern yew Taxuschinensis is a plant of the genus Taxus (Taxus) in the subphylum Cymnospermai, the class Coniferopside, the order Taxales, and the family Taxaceae. It is a unique Quaternary relic plant in my country. Distributed in the Yangtze River Basin, Nanling Mountains and mountains or valleys in Henan, Shaanxi, Qinling, Gansu and other provinces. Southern yew has both important ornamental value and important medicinal value. Paclitaxel contained in it is an important drug for treating cancer. Due to the low natural reproduction and regeneration capacity and excessive deforestation by humans, the number of southern yews has become less and less, and it is currently in an endangered state. During the seedling period, the stem...
Claims
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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895
Inventor 沈晨佳冯尚国王慧中
Owner HANGZHOU NORMAL UNIVERSITY